Uncovering mechanisms of greengage wine fermentation against acidic stress via genomic, transcriptomic, and metabolic analyses of Saccharomyces cerevisiae

Tian, Tiantian; Wu, Dianhui; Ng, Chan-Tat; Yang, Hua; Liu, Jun; Sun, Jiazeng; Lü, Jing

doi:10.1007/s00253-020-10772-z

Cited by 9 publications

(3 citation statements)

References 30 publications

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“…Previous studies applying ALE have evolved in favor of various S . cerevisiae phenotypes, such as tolerance to inhibiting concentrations of organic acids [ 28 , 40 ], stress resistance [ 41 ] and high temperature [ 42 ]. In our study, ALE was used to guarantee yeast cell growth under high 2-PE concentration stress and to enhance the synthesis of 2-PE production.…”

Section: Discussionmentioning

confidence: 99%

Improvement of 2-phenylethanol production in Saccharomyces cerevisiae by evolutionary and rational metabolic engineering

Zhu

et al. 2021

PLoS ONE

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2-Phenylethanol (2-PE) is a valuable aromatic compound with favorable flavors and good properties, resulting in its widespread application in the cosmetic, food and medical industries. In this study, a mutant strain, AD032, was first obtained by adaptive evolution under 2-PE stress. Then, a fusion protein from the Ehrlich pathway, composed of tyrB from Escherichia coli, kdcA from Lactococcus lactis and ADH2 from Saccharomyces cerevisiae, was constructed and expressed. As a result, 3.14 g/L 2-PE was achieved using L-phenylalanine as a precursor. To further increase 2-PE production, L-glutamate oxidase from Streptomyces overexpression was applied for the first time in our research to improve the supply of α-ketoglutarate in the transamination of 2-PE synthesis. Furthermore, we found that the disruption of the pyruvate decarboxylase encoding gene PDC5 caused an increase in 2-PE production, which has not yet been reported. Finally, assembly of the efficient metabolic modules and process optimization resulted in the strain RM27, which reached 4.02 g/L 2-PE production from 6.7 g/L L-phenylalanine without in situ product recovery. The strain RM27 produced 2-PE (0.8 mol/mol) with L-phenylalanine as a precursor, which was considerably high, and displayed manufacturing potential regarding food safety and process simplification aspects. This study suggests that innovative strategies regarding metabolic modularization provide improved prospects for 2-PE production in food exploitation.

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Section: Discussionmentioning

confidence: 99%

Improvement of 2-phenylethanol production in Saccharomyces cerevisiae by evolutionary and rational metabolic engineering

Zhu

et al. 2021

PLoS ONE

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“…have been changed in S. cerevisiae in response to acid exposure and metabolic profile analysis confirmed these transcriptional changes. , Additionally, the transcriptional regulating networks in S. cerevisiae were shown to control other cellular processes and play key roles in metabolism, environmental responses, development, etc .…”

Section: Resultsmentioning

confidence: 80%

“…have been changed in S. cerevisiae in response to acid exposure and metabolic profile analysis confirmed these transcriptional changes. 53,54 Additionally, the transcriptional regulating networks in S. cerevisiae were shown to control other cellular processes and play key roles in metabolism, environmental responses, development, etc. 55 Nonetheless, as the RNA-Seq analysis can only provide information on the transcription, associated non-volatile intermediate metabolites and enzyme activity changes still need to be further confirmed in subsequent studies.…”

Section: ■ Statistical Analysismentioning

confidence: 99%

Gene-Regulated Release of Distinctive Volatile Organic Compounds from Stressed Living Cells

Chen

Zheng

Wang

et al. 2022

Environ. Sci. Technol.

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Breath-borne volatile organic compounds (VOCs) have been increasingly studied as non-invasive biomarkers in both medical diagnosis and environmental health research. Recently, changes in breath-borne VOC fingerprints were demonstrated in rats and humans following pollutant exposures. In this study, the eukaryotic model Saccharomyces cerevisiae was used to study the release of cellular VOCs resulting from toxicant exposures (i.e., O3, H2O2, and CO2) and its underlying biological mechanism. Our results showed that different toxicant exposures caused the release of distinctive VOC profiles of yeast cells. The levels of ethyl acetate and ethyl n-propionate were altered in response to all the toxicants used in this study and could thus be targeted for future environmental toxicity monitoring. The RNA-seq results revealed significant changes in the metabolic or signaling pathways related to the ribosome, carbohydrate, and amino acid metabolisms after exposures. Notably, the shift from glycolysis to the pentose phosphate pathway of carbohydrate metabolism and the inhabitation of the aspartate pathway in the lysine synthesis was essential to the cellular antioxidation by providing reduced nicotinamide adenine dinucleotide phosphate (NADPH). The reprogrammed metabolisms could have resulted in the observed changes of VOCs released, e.g., the production of ethyl acetate for detoxification from yeast cells. This study provides further evidence that VOCs released from living organisms could be used to monitor and guard against toxic exposures while providing better mechanistic insights of the changes in breath-borne VOCs previously observed in rats and humans exposed to air toxicants.

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Exploring the stress mechanism of tannic acid on Saccharomyces cerevisiae based on transcriptomics

Li,

Deng,

Chen

et al. 2023

Food Bioscience

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Uncovering mechanisms of greengage wine fermentation against acidic stress via genomic, transcriptomic, and metabolic analyses of Saccharomyces cerevisiae

Cited by 9 publications

References 30 publications

Improvement of 2-phenylethanol production in Saccharomyces cerevisiae by evolutionary and rational metabolic engineering

Improvement of 2-phenylethanol production in Saccharomyces cerevisiae by evolutionary and rational metabolic engineering

Gene-Regulated Release of Distinctive Volatile Organic Compounds from Stressed Living Cells

Exploring the stress mechanism of tannic acid on Saccharomyces cerevisiae based on transcriptomics

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