Uncovering proteome variations of differently heat-treated sea cucumber (Apostichopus japonicus) by label-free mass spectrometry

Jiang, Bingxue; Hu, Lingping; Zhang, Xiaomei; Zhang, Hongwei; Zhang, Feng; Chen, Lipin; Li, Zhaojie; Zhao, Xue; Xue, Changhu; Jiang, Xiaoming

doi:10.1016/j.foodchem.2020.128575

Cited by 24 publications

(4 citation statements)

References 33 publications

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“…Mass spectrometry is one of the most effective quantitative analysis platforms with the advantages of robustness, sensitivity, selectivity, multiplexing, and high throughput [ 32 ]. Quantitative proteomics based on LC-MS/MS has become an important method to identify and quantify protein abundance.…”

Section: Discussionmentioning

confidence: 99%

Serum exosomal pregnancy zone protein as a promising biomarker in inflammatory bowel disease

et al. 2021

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Background Inflammatory bowel disease (IBD) is a kind of intestinal immune dysfunction disease, and its occurrence and prevalence are on the rise worldwide. As a chronic gastrointestinal disease, its pathogenesis is still unknown. Exosomes are vesicles in various body fluids that carry a variety of substances. They can mediate intercellular communication and long-distance transport of multiple media. In this study, we investigated the protein profile of serum exosomes from healthy people and IBD patients to explore a new serological biomarker for IBD. Methods Initially, exosomes were extracted from serum samples, and the proteins within the exosomes were identified by label-free liquid chromatography/mass spectrometry (LC-MS/MS). Western blot and ELISA were used to assess the identified protein. To further analyze the target protein, an acute colitis mouse model was established, and exosomes in colonic tissue and serum were extracted to investigate the protein in them. Results Firstly, serum exosomes were extracted from samples, and proteins in exosomes were identified by LC-MS/MS. Through statistical analysis, we identified 633 proteins. Among these proteins, pregnancy zone protein (PZP) showed a marked difference between patients with IBD and healthy people, in that its expression level was much higher in the IBD patients This exosomal protein was associated with immunosuppressive effects. Also, the level of PZP in colon tissue exosomes and serum exosomes of acute colitis mice was significantly higher than that of the control group. Conclusions Our findings indicated that serum exosome PZP was present at a high level in the IBD patients. Hence it might be a promising biomarker and enhance auxiliary diagnosis of IBD.

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Section: Discussionmentioning

confidence: 99%

Serum exosomal pregnancy zone protein as a promising biomarker in inflammatory bowel disease

et al. 2021

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“…The data acquisition method used was data-dependent acquisition (DDA) in positive ion mode. , DDA data was analyzed using ProteinPilot (version 5.0.1, SCIEX, Framingham, MA, USA) software for database search. The reference database was sourced from the NCBI Web site (as of August 9, 2022, 52164 tropomyosin proteins were retrieved).…”

Section: Materials and Methodsmentioning

confidence: 99%

Quantification of Allergic Crustacean Tropomyosin Using Shared Signature Peptides in Processed Foods with a Mass Spectrometry-Based Proteomic Strategy

Lu,

Zhang,

Gao

et al. 2024

J. Agric. Food Chem.

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Crustacean shellfish are major allergens in East Asia. In the present study, a major allergic protein in crustaceans, tropomyosin, was detected accurately using multiple reaction monitoring mode-based mass spectrometry, with shared signature peptides identified through proteomic analysis. The peptides were deliberately screened through thermal stability and enzymatic digestion efficiency to improve the suitability and accuracy of the developed method. Finally, the proposed method demonstrated a linear range of 0.15 to 30 mg TM /kg food (R 2 > 0.99), with a limit of detection of 0.15 mg TM /kg food and a limit of quantification of 0.5mg TM /kg food and successfully applied to commercially processed foods, such as potato chips, biscuits, surimi, and hot pot seasonings, which evidenced the applicability of proteomics-based methodology for food allergen analysis.

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“…Proteomic analysis based on the Sequential window acquisition of all theoretical fragment ion mass spectrometry (SWATH‐MS) analysis was integrated to reveal the relative quantitative expression of HSP70 and cytochrome c protein for different salinity depuration of C. gigas according to our previous studies 1,27 . First, the tryptic digests of C. gigas were analyzed using data‐dependent acquisition (DDA) strategy.…”

Section: Methodsmentioning

confidence: 99%

Effect of salinity stress on respiratory metabolism, glycolysis, lipolysis, and apoptosis in Pacific oyster (Crassostrea gigas) during depuration stage

Chen

Shi

et al. 2021

J Sci Food Agric

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BACKGROUND Depuration is an important process performed to ensure the safety of oyster consumption, and the effect of salinity stress on physiological and ecological characteristics of oyster remains unknow. In this study, the simulated depuration of Crassostrea gigas was performed with the salinities varying from ±10% to ±20% away from that of production area (26, 28, 32, 35, and 38 g L–1), as well as respiratory metabolism, glycolysis, lipolysis, and apoptosis were analyzed. RESULTS (i) The oxygen consumption rate, ammonia discharge rate and enzyme activities related to respiratory metabolism were decreased significantly at salinities of 38 g L–1, indicating that salinity stress triggered the abnormal respiratory metabolism of C. gigas, further, glycolysis was enhanced. (ii) Glycogen decomposition, lactic acid increase, and fatty acid composition modifications were caused by adenosine monophosphate (AMP)‐activated protein kinase (AMPK) ‐mediated during salinity stress. (iii) There was a clear decrease of the condition index and meat yield of C. gigas after 72 h of depuration, especially in salinity 38 g L–1. (iv) Salinity stress would lead to the increase of cytochrome c levels, then cause apoptosis of C. gigas, while heat shock protein 70 (HSP70) would interfere with this process. CONCLUSION Salinity stress had a significant effect on the physiological and ecological response of C. gigas during the depuration process, including respiratory metabolism, glycolysis, lipolysis, and apoptosis. In general, the low depuration salinity fluctuation (±10%) is helpful to maintain quality of C. gigas, as well as the optimal depuration time was 48 h. © 2021 Society of Chemical Industry.

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Uncovering proteome variations of differently heat-treated sea cucumber (Apostichopus japonicus) by label-free mass spectrometry

Cited by 24 publications

References 33 publications

Serum exosomal pregnancy zone protein as a promising biomarker in inflammatory bowel disease

Serum exosomal pregnancy zone protein as a promising biomarker in inflammatory bowel disease

Quantification of Allergic Crustacean Tropomyosin Using Shared Signature Peptides in Processed Foods with a Mass Spectrometry-Based Proteomic Strategy

Effect of salinity stress on respiratory metabolism, glycolysis, lipolysis, and apoptosis in Pacific oyster (Crassostrea gigas) during depuration stage

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