Uncovering the dominant role of root metabolism in shaping rhizosphere metabolome under drought in tropical rainforest plants

Hildebrand, Gina; Honeker, Linnea K.; Freire-Zapata, Viviana; Ayala-Ortiz, Christian; Rajakaruna, Sumudu; Fudyma, Jane; Daber, Lars Erik; AminiTabrizi, Roya; Chu, Rosalie K.; Toyoda, Jason; Flowers, Sarah E.; Hoyt, David; Hamdan, Rasha; Gil-Loaiza, Juliana; Shi, Lingling; Dippold, Michaela A.; Ladd, S. Nemiah; Werner, Christiane; Meredith, Laura; Tfaily, Malak M.

doi:10.1016/j.scitotenv.2023.165689

Cited by 6 publications

(3 citation statements)

References 99 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recently proposed assembly approach, entitled infer community assembly mechanisms by phylogenetic bin-based null model analysis (iCAMP), was used to evaluate the contribution of ecological processes of the alpine soil potential diazotrophic community assembly ( 70 ). OTUs were first divided into bins based on their phylogenetic relationships created by using FastTree ( 71 ). The relative contributions of heterogeneous selection, homogeneous selection, homogenizing dispersal, drift, and dispersal limitation in each bin were measured using the within-bin beta net relatedness index (βNRI) and modified Raup-Crick metric (RC Bray ).…”

Section: Methodsmentioning

confidence: 99%

Effect of aridity on the β-diversity of alpine soil potential diazotrophs: insights into community assembly and co-occurrence patterns

Lei,

Wang,

Wang

et al. 2024

mSystems

View full text Add to dashboard Cite

Microbial diversity plays a vital role in the maintenance of ecosystem functions. However, the current understanding of mechanisms that shape microbial diversity along environmental gradients at broad spatial scales is relatively limited, especially for specific functional groups, such as potential diazotrophs. Here, we conducted an aridity-gradient transect survey from 60 sites across the Tibetan Plateau, the largest alpine ecosystem of the planet, to investigate the ecological processes (e.g., local species pools, community assembly processes, and co-occurrence patterns) that underlie the β-diversity of alpine soil potential diazotrophic communities. We found that aridity strongly and negatively affected the abundance, richness, and β-diversity of soil diazotrophs. Diazotrophs displayed a distance-decay pattern along the aridity gradient, with organisms living in lower aridity habitats having a stronger distance-decay pattern. Arid habitats had lower co-occurrence complexity, including the number of edges and vertices, the average degree, and the number of keystone taxa, as compared with humid habitats. Local species pools explained limited variations in potential diazotrophic β-diversity. In contrast, co-occurrence patterns and stochastic processes (e.g., dispersal limitation and ecological drift) played a significant role in regulating potential diazotrophic β-diversity. The relative importance of stochastic processes and co-occurrence patterns changed with increasing aridity, with stochastic processes weakening whereas that of co-occurrence patterns enhancing. The genera Geobacter and Paenibacillus were identified as keystone taxa of co-occurrence patterns that are associated with β-diversity. In summary, aridity affects the co-occurrence patterns and community assembly by regulating soil and vegetation characteristics and ultimately shapes the β-diversity of potential diazotrophs. These findings highlight the importance of co-occurrence patterns in structuring microbial diversity and advance the current understanding of mechanisms that drive belowground communities. IMPORTANCE Recent studies have shown that community assembly processes and species pools are the main drivers of β-diversity in grassland microbial communities. However, co-occurrence patterns can also drive β-diversity formation by influencing the dispersal and migration of species, the importance of which has not been reported in previous studies. Assessing the impact of co-occurrence patterns on β-diversity is important for understanding the mechanisms of diversity formation. Our study highlights the influence of microbial co-occurrence patterns on β-diversity and combines the drivers of community β-diversity with drought variation, revealing that drought indirectly affects β-diversity by influencing diazotrophic co-occurrence patterns and community assembly.

show abstract

Section: Methodsmentioning

confidence: 99%

Effect of aridity on the β-diversity of alpine soil potential diazotrophs: insights into community assembly and co-occurrence patterns

Lei,

Wang,

Wang

et al. 2024

mSystems

View full text Add to dashboard Cite

show abstract

“…Thus, drought stress significantly alters root traits and metabolite profiles in plants, profoundly influencing the rhizosphere microbiome (Figure 4). Drought-induced changes in the rhizosphere microbiome are partly governed by plant-specific responses, especially in root characteristics, including changes in root morphology or shifts in belowground carbon distribution patterns, crucial for forest trees adaptation to drought (Ma et al, 2018;Hildebrand et al, 2023). For example, in a forest ecosystem, metabolomic analyses of the Populus trichocarpa plant have shown that drought conditions increase the plant's investment in carbon (C) and nitrogen (N) metabolisms, as evidenced by the root tissue profiles of amino acids, fatty acids, and phenolic glycosides (Veach et al, 2020) and thus tolerance to drought condition.…”

Section: Drought Effectsmentioning

confidence: 99%

“…However, this assemblage of microbes in different soil horizons may change due to the changes in root morphology and exudation of forest trees along a wide range of environmental gradients, for example drought or temperature (Meier et al, 2020;Veach et al, 2020). It has even been hypothesized that plants dynamically recruit soil microbes by secreting metabolites in the rhizosphere that ideally stimulate rhizosphere microorganisms or endophytic fungi and bacteria that are advantageous to plant growth by helping them cope with abiotic stresses (Sasse et al, 2018;Hildebrand et al, 2023). Knowledge about the responses of root metabolites and microbial assemblages in the roots and rhizosphere in different horizons of forest soil along environmental gradients of drought and temperature will increase our aptitude to forecast the global change effects on soil organic matter decomposition and C cycling (Steidinger et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Metabolic niches in the rhizosphere microbiome: dependence on soil horizons, root traits and climate variables in forest ecosystems

Maitra,

Hrynkiewicz,

Szuba

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

Understanding belowground plant-microbial interactions is important for biodiversity maintenance, community assembly and ecosystem functioning of forest ecosystems. Consequently, a large number of studies were conducted on root and microbial interactions, especially in the context of precipitation and temperature gradients under global climate change scenarios. Forests ecosystems have high biodiversity of plants and associated microbes, and contribute to major primary productivity of terrestrial ecosystems. However, the impact of root metabolites/exudates and root traits on soil microbial functional groups along these climate gradients is poorly described in these forest ecosystems. The plant root system exhibits differentiated exudation profiles and considerable trait plasticity in terms of root morphological/phenotypic traits, which can cause shifts in microbial abundance and diversity. The root metabolites composed of primary and secondary metabolites and volatile organic compounds that have diverse roles in appealing to and preventing distinct microbial strains, thus benefit plant fitness and growth, and tolerance to abiotic stresses such as drought. Climatic factors significantly alter the quantity and quality of metabolites that forest trees secrete into the soil. Thus, the heterogeneities in the rhizosphere due to different climate drivers generate ecological niches for various microbial assemblages to foster beneficial rhizospheric interactions in the forest ecosystems. However, the root exudations and microbial diversity in forest trees vary across different soil layers due to alterations in root system architecture, soil moisture, temperature, and nutrient stoichiometry. Changes in root system architecture or traits, e.g. root tissue density (RTD), specific root length (SRL), and specific root area (SRA), impact the root exudation profile and amount released into the soil and thus influence the abundance and diversity of different functional guilds of microbes. Here, we review the current knowledge about root morphological and functional (root exudation) trait changes that affect microbial interactions along drought and temperature gradients. This review aims to clarify how forest trees adapt to challenging environments by leveraging their root traits to interact beneficially with microbes. Understanding these strategies is vital for comprehending plant adaptation under global climate change, with significant implications for future research in plant biodiversity conservation, particularly within forest ecosystems.

show abstract

Harnessing Belowground Interaction: Re-analyzing the Role of Rhizosphere Microbiome in Plant–Pathogen Interaction Under Water Stress

Gupta,

Sinha,

Bhar

2024

J Plant Growth Regul

View full text Add to dashboard Cite

Uncovering the dominant role of root metabolism in shaping rhizosphere metabolome under drought in tropical rainforest plants

Cited by 6 publications

References 99 publications

Effect of aridity on the β-diversity of alpine soil potential diazotrophs: insights into community assembly and co-occurrence patterns

Effect of aridity on the β-diversity of alpine soil potential diazotrophs: insights into community assembly and co-occurrence patterns

Metabolic niches in the rhizosphere microbiome: dependence on soil horizons, root traits and climate variables in forest ecosystems

Harnessing Belowground Interaction: Re-analyzing the Role of Rhizosphere Microbiome in Plant–Pathogen Interaction Under Water Stress

Contact Info

Product

Resources

About