Uncovering the overcapacity feature of China's industry and the environmental &amp; health co-benefits from de-capacity

Jiawen, Guo; Dong, Huijuan; Farzaneh, Hooman; Geng, Yong; Reddington, Carly L.

doi:10.1016/j.jenvman.2022.114645

Cited by 21 publications

(11 citation statements)

References 36 publications

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“…n -acetylglutamate can be obtained with a yield of 98% by mixing acetic anhydride, l -glutamate, and water into ultrasonic waves for 10 mins at ambient temperature [ 24 , 25 ]. In the past decades, the improvement in l -glutamate fermentation technology leads to a continued decline in its price [ 26 ], on the other hand, acetic anhydride is a bulk chemical with a price of ~$0.5/kg [ 27 ]. Therefore, we envision this hybrid approach is economically feasible to replace the endogenous biosynthesis of n -acetylglutamate and potentially overcome the loss of carbon yield in the l -arginine biosynthesis pathway.…”

Section: Introductionmentioning

confidence: 99%

A novel strategy for l-arginine production in engineered Escherichia coli

2023

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Background l-arginine is an important amino acid with applications in diverse industrial and pharmaceutical fields. n-acetylglutamate, synthesized from l-glutamate and acetyl-CoA, is a precursor of the l-arginine biosynthetic branch in microorganisms. The enzyme that produces n-acetylglutamate, n-acetylglutamate synthase, is allosterically inhibited by l-arginine. l-glutamate, as a central metabolite, provides carbon backbone for diverse biological compounds besides l-arginine. When glucose is the sole carbon source, the theoretical maximum carbon yield towards l-arginine is 96.7%, but the experimental highest yield was 51%. The gap of l-arginine yield indicates the regulation complexity of carbon flux and energy during the l-arginine biosynthesis. Besides endogenous biosynthesis, n-acetylglutamate, the key precursor of l-arginine, can be obtained by chemical acylation of l-glutamate with a high yield of 98%. To achieve high-yield production of l-arginine, we demonstrated a novel approach by directly feeding precursor n-acetylglutamate to engineered Escherichia coli. Results We reported a new approach for the high yield of l-arginine production in E. coli. Gene argA encoding n-acetylglutamate synthase was deleted to disable endogenous biosynthesis of n-acetylglutamate. The feasibility of external n-acetylglutamate towards l-arginine was verified via growth assay in argA− strain. To improve l-arginine production, astA encoding arginine n-succinyltransferase, speF encoding ornithine decarboxylase, speB encoding agmatinase, and argR encoding an arginine responsive repressor protein were disrupted. Based on overexpression of argDGI, argCBH operons, encoding enzymes of the l-arginine biosynthetic pathway, ~ 4 g/L l-arginine was produced in shake flask fermentation, resulting in a yield of 0.99 mol l-arginine/mol n-acetylglutamate. This strain was further engineered for the co-production of l-arginine and pyruvate by removing genes adhE, ldhA, poxB, pflB, and aceE, encoding enzymes involved in the conversion and degradation of pyruvate. The resulting strain was shown to produce 4 g/L l-arginine and 11.3 g/L pyruvate in shake flask fermentation. Conclusions Here, we developed a novel approach to avoid the strict regulation of l-arginine on ArgA and overcome the metabolism complexity in the l-arginine biosynthesis pathway. We achieve a high yield of l-arginine production from n-acetylglutamate in E. coli. Co-production pyruvate and l-arginine was used as an example to increase the utilization of input carbon sources.

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Section: Introductionmentioning

confidence: 99%

A novel strategy for l-arginine production in engineered Escherichia coli

2023

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“…Drawing on the idea of Guo et al (2022) [58], this paper uses DEA to measure urban capacity utilization rate (CU) and regards capacity utilization rate as a reverse indicator to measure the degree of overcapacity. From the results in Column (3) of table 7, it can be seen that the national-level entrepreneurial city pilot program has a significantly negative effect on the urban capacity utilization rate, which indicates that the national-level entrepreneurial city pilot program is not conducive to the improvement of urban capacity utilization rate, that is, it aggravates overcapacity.…”

Section: Exacerbating Overcapacitymentioning

confidence: 99%

Entrepreneurship promotion policies and urban carbon emission efficiency: does environmental concerns matter?

He,

Zhou

2024

Environ. Res. Commun.

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To foster economic and social development, many countries have adopted entrepreneurship promotion policies. However, existing research on entrepreneurship promotion policy primarily focuses on its employment incentive effect, while neglecting to examine the underlying impact mechanism and its effect on environmental sustainability. Based on the quasi-natural experiment of China's National Entrepreneurial Pilot Cities, this paper employs the difference-in-differences model to examine the impact of the National Entrepreneurial Cities pilot policy on urban carbon emission efficiency. The study finds that China's National Entrepreneurial Cities pilot policy has generally impaired urban carbon emission efficiency, and a series of robustness tests show that the results are robust. National Entrepreneurial Cities pilot policy decreases urban carbon emission efficiency by increasing the proportion of necessity entrepreneurship, inhibiting the upgrading of industrial structure, and increasing overcapacity. The government's environmental concerns and the public's environmental concerns play a prominent role in improving the adverse impact of the National Entrepreneurial Cities on carbon emission efficiency and mainly play a significant role in central and western cities, cities with low per capita GDP, cities with high economic growth pressure, and resource-dependent cities. The research of this paper provides a reference for the government to promote entrepreneurship while taking environmental effects into account.

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“…The data sources of this paper are as follows: (1) This paper takes industrial enterprises listed in Shanghai and Shenzhen A-shares from 2015-2019 as the sample (according to the industry classification of the China Securities Regulatory Commission, enterprises in the industrial sector are those in the following industries: mining, manufacturing, electricity, heat, gas and water production, and supply). The following screening and processing of the sample and data were carried out: (1) exclude the samples of companies that were specially treated such as ST and SST, etc.…”

Section: Data Source and Sample Processingmentioning

confidence: 99%

“…Over the past 40 years of reform and opening-up, China's economic development has achieved world-renowned successes, but the sloppy economic development model has not only increased the burden on the ecological environment, it has also made the problem of overcapacity increasingly prominent [1]. Different degrees of overcapacity are experienced by 42.8% of 35 industries [2].…”

Section: Introductionmentioning

confidence: 99%

Environmental “Fee-to-Tax” and Heavy Pollution Enterprises to De-Capacity

Zeng

2022

Sustainability

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Will environmental “fee-to-tax”, which strengthens the rigidity of levies and increases the environmental costs of heavy pollution enterprises, force heavy pollution enterprises to de-capacity. This paper examines the impact and heterogeneity of the environmental “fee-to-tax” on heavy pollution enterprises’ de-capacity by taking the official implementation of the Environmental Protection Tax Law of the People’s Republic of China in 2018 as the institutional impact and the listed industrial enterprises in Shanghai and Shenzhen A-shares from 2015 to 2019 as the sample. The study found that environmental “fee-to-tax” pushed heavy pollution enterprises to de-capacity. After parallel trend tests, placebo tests, eliminate policy interference, propensity score matching, and replacement variables, the findings of this paper are robust. Further analysis shows that the effect of environmental “fee-to-tax” on heavy pollution enterprises’ de-capacity is more significant in state-owned enterprises, high financing constraints enterprises, and areas with higher tax collection and management; this effect is still effective and more significant in areas with low economic development because all environmental tax revenues are included in local finance. This paper follows up and tests the implementation effect of environmental regulations in China and provides a valuable reference for the government to promote the task of de-capacity and green transformation and upgrading of industrial structure through actively playing the role of environmental protection.

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Uncovering the overcapacity feature of China's industry and the environmental & health co-benefits from de-capacity

Cited by 21 publications

References 36 publications

A novel strategy for l-arginine production in engineered Escherichia coli

A novel strategy for l-arginine production in engineered Escherichia coli

Entrepreneurship promotion policies and urban carbon emission efficiency: does environmental concerns matter?

Environmental “Fee-to-Tax” and Heavy Pollution Enterprises to De-Capacity

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