Uncovering the prominent role of satellite cells in paravertebral muscle development and aging by single-nucleus RNA sequencing

Qiu, Xin; Wang, Haoyu; Yang, Zhenyu; Sun, Li-Ming; Liu, Shu-Nan; Fan, Chuiqin; Zhu, Feng

doi:10.1016/j.gendis.2023.01.005

Cited by 7 publications

(3 citation statements)

References 74 publications

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“…Furthermore, we demonstrated the involvement of the MEF2A-MEG3/DIO3-PP2A signaling cascade in myoblast differentiation [ 17 ]. Mef2a is highly expressed in satellite cells, regulates Pdha1 expression and is associated with skeletal muscle development [ 26 ]. The Smads family of proteins plays a crucial role in the TGF-β signaling pathway by transferring signals from the cell membrane to the nucleus, thereby modulating the transcription of target genes.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis reveals genomic diversity and signatures of selection in Qinchuan beef cattle

Yu,

Zhang,

Cheng

et al. 2024

BMC Genomics

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Background Indigenous Chinese cattle have abundant genetic diversity and a long history of artificial selection, giving local breeds advantages in adaptability, forage tolerance and resistance. The detection of selective sweeps and comparative genome analysis of selected breeds and ancestral populations provide a basis for understanding differences among breeds and for the identification and utilization of candidate genes. We investigated genetic diversity, population structure, and signatures of selection using genome-wide sequencing data for a new breed of Qinchuan cattle (QNC, n = 21), ancestral Qinchuan cattle (QCC, n = 20), and Zaosheng cattle (ZSC, n = 19). Results A population structure analysis showed that the ancestry components of QNC and ZSC were similar. In addition, the QNC and ZSC groups showed higher proportions of European taurine ancestry than that of QCC, and this may explain the larger body size of QNC, approaching that of European cattle under long-term domestication and selection. A neighbor-joining tree revealed that QCC individuals were closely related, whereas QNC formed a distinct group. To search for signatures of selection in the QNC genome, we evaluated nucleotide diversity (θπ), the fixation index (FST) and Tajima’s D. Overlapping selective sweeps were enriched for one KEGG pathway, the apelin signaling pathway, and included five candidate genes (MEF2A, SMAD2, CAMK4, RPS6, and PIK3CG). We performed a comprehensive review of genomic variants in QNC, QCC, and ZSC using whole-genome sequencing data. QCC was rich in novel genetic diversity, while diversity in QNC and ZSC cattle was reduced due to strong artificial selection, with divergence from the original cattle. Conclusions We identified candidate genes associated with production traits. These results support the success of selective breeding and can guide further breeding and resource conservation of Qinchuan cattle.

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Section: Discussionmentioning

confidence: 99%

Genome-wide analysis reveals genomic diversity and signatures of selection in Qinchuan beef cattle

Yu,

Zhang,

Cheng

et al. 2024

BMC Genomics

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“…PGAM2, an essential enzyme, participates in the glycolytic pathway, and its content A c c e p t e d A r t i c l e reflects the state of the tricarboxylic acid cycle [46]. Wojtusik et al reported that CLC treatment of gazelle sperm reduced the effect of freezing on sperm glycolysis by reducing the degradation of PGAM2 [47].…”

Section: A C C E P T E D a R T I C L Ementioning

confidence: 99%

Effect of cholesterol-loaded cyclodextrin treatment on boar sperm cryopreservation

Zhang,

Liu

et al. 2024

Anim Biosci

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Objective: This study investigated the efficacy of different concentrations of Cholesterol-loaded cyclodextrin (CLC) on cryopreservation in boar sperm quality. Methods:In this study, we treated boar sperm with different concentrations of CLC before freezing and analyzed the sperm cholesterol concentration, plasma membrane, acrosome integrity rate and total motility rate before and after freeze-thawing. We also investigated the levels of reactive oxygen species (ROS), malondialdehyde (MDA), ATP, and structural-and oxidative-damage related proteins in all groups after thawing. Results:The results revealed that the cholesterol concentration of the CLC-treated groups was higher than that of the control group, both before freezing and after thawing (p < 0.05). The plasma membrane integrity rate, acrosome integrity rate, and total motility rate of sperm were also enhanced after thawing in the CLC-treated group (all p < 0.05). Moreover, ROS and MDA production and ATP loss were reduced in CLC-treated sperm during freezing and thawing (p < 0.05). Finally, CLC pretreatment partially prevented the consumption of various proteins involved in metabolism including CAPZB, HSP90AA1 and PGAM2 (p < 0.05).

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“…In PMD, a persistent imbalance between protein synthesis and degradation leads to a decline in skeletal muscle mass and function, signi cantly impacting morbidity and mortality rates. Unfortunately, there are currently no established pharmaceutical treatments for PMDinduced skeletal muscle dysfunction [14][15][16]. Numerous studies have emphasized that in ammatory in ltration, mitochondrial dysfunction and oxidative stress play an important role in PMD [17,18].…”

Section: Introductionmentioning

confidence: 99%

Naringin Ameliorates IL-6 Mediated mitochondrial dysfunction and skeletal muscle degeneratio via AMPK/Nrf-2 pathway

Gao,

Wang,

Zhang

et al. 2024

Preprint

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Ethnopharmacological relevance: Paraspinal muscle degeneration often arises as a hazardous consequence of intervertebral disc degeneration (IVDD). This degeneration correlates with oxidative stress and mitochondrial dysfunction. This study investigated the therapeutic potential of naringin in managing paraspinal muscle degeneration associated with disc degeneration. Materials and methods: C2C12 cells were stimulated with IL-6 to establish an in vitro model of skeletal muscle degeneration for assessing the protective impact of naringin on skeletal muscle. The most effective concentration of naringin in C2C12 cells was identified through a CCK8 assay. The antioxidant prowess of naringin was evaluated via biochemical methods and Elisa. The influences of naringin and IL-6 on apoptosis, mitochondrial function, and associated signaling pathways were examined using cytometry, ROS detection, western blot, and transmission electron microscopy. Results: Our findings demonstrated a significant reduction in discogenic paraspinal degeneration with naringin therapy. Naringin glycosides notably enhanced the expression of key proteins involved in both muscle anabolism and catabolism, including MAFbx, MuRF1, MyoD, and MyoG.Moreover, naringin contributed to maintaining redox homeostasis by augmenting antioxidant activity and preventing excessive ROS peroxide accumulation. To impede paraspinal muscle degeneration, naringin upregulated MyoD and MyoG expression while downregulating MAFbx and MuRF1 through the activation of AMPK/Nrf-2 signaling pathway. Conclusion: These findings underscore naringin's robust therapeutic potential in enhancing mitochondrial activity, regulating oxidative stress, and halting paraspinal muscle degeneration.

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Uncovering the prominent role of satellite cells in paravertebral muscle development and aging by single-nucleus RNA sequencing

Cited by 7 publications

References 74 publications

Genome-wide analysis reveals genomic diversity and signatures of selection in Qinchuan beef cattle

Genome-wide analysis reveals genomic diversity and signatures of selection in Qinchuan beef cattle

Effect of cholesterol-loaded cyclodextrin treatment on boar sperm cryopreservation

Naringin Ameliorates IL-6 Mediated mitochondrial dysfunction and skeletal muscle degeneratio via AMPK/Nrf-2 pathway

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