Uncovering the role of DNA methyltransferases in grapevine – Plasmopara viticola interaction: From genome-wide characterization to global methylation patterns

Transcriptomic and methylation analysis of susceptible and tolerant grapevine genotypes following Plasmopara viticola infection

“…Also, studies have observed that altering DNA methylation levels (hypo‐ or hypermethylation) on genomic regions could influence gene and phenotypic responses to plant diseases (Geng et al, 2019 ; Leonetti & Molinari, 2020 ). Our results are in agreement with recent work done by Pereira and authors, which reported that the global cytosine analysis performed on the tolerant cultivar Regent revealed a decrease of global methylation levels in the early hours of infection by P. viticola in comparison to the Trincadeira, a susceptible genotype (Pereira et al, 2022 ).…”

“…The percentage of global 5‐mC methylation for each sample was calculated as fold change of inoculated versus mock (control). Statistical analysis was also performed to compare the fold change between N20/020 and N23/018 at each time‐point, using a Welch's t test from GraphPad Prism software version 8.2.1 (Pereira et al, 2022 ).…”

“…The contribution of P. viticola DNA methylation was not taken into account for the global methylation calculations, as in Pereira et al ( 2022 ). It is reported that in oomycetes the methylation of adenines (6 mA) is the more frequent DNA methylation modification (Atighi et al, 2020 ; Chen et al, 2018 ; Pereira et al, 2022 ).…”

“…How to cite this article: Azevedo, V., Daddiego, L., Cardone, M.F., Perrella, G., Sousa, L., Santos, R.B. et al (2022) Transcriptomic and methylation analysis of susceptible and tolerant grapevine genotypes following Plasmopara viticola infection.…”

Emerging Roles of Epigenetics in Grapevine and Winegrowing

“…DNA methylation sequence context and nine related DNTMs have been recently identified in grapevine ( Figure 1 ). These grapevine DNTMs code for seventeen proteins due to alternative splicing [ 25 ]. MET genes undergoing alternative splicing have also been reported in rice [ 26 ].…”