Uncultured adipose-derived regenerative cells (ADRCs) seeded in collagen scaffold improves dermal regeneration, enhancing early vascularization and structural organization following thermal burns

Adipose-Derived Regenerative Cell Therapy for Burn Wound Healing: A Comparison of Two Delivery Methods

“…Similarly, numerous preclinical and case study reports from our group and others have reported the angiogenic effect of ADRCs in several acute and chronic injury models. 11,26,27,33 Taken together, these data suggest that ADRCs promote tissue repair through their effect on modulating the inflammatory response and promoting angiogenesis and epithelialization.…”

“…5,11,12,18,[26][27][28] Nevertheless, a current problem in the development of such therapies is to optimize the delivery approaches so that the cell suspension efficiently reaches the wound beds. Currently, there are two basic delivery methods for acute and chronic wounds: local injections around the perimeter of the wounds and topical application.…”

“…11 Briefly, six full-thickness burns (*10 cm 2 each) were created on the dorsum of each animal using a custommade burn induction device. Two days postinjury, wound sites were excised to the level of the underlying muscular fascia.…”

“…8,9 We have referred to clinical grade SVF as adipose-derived regenerative cells (ADRCs). 10,11 Studies in renal and cardiovascular injury models have described a beneficial role of ADRCs during repair that appears to be mediated, at least in part, by modulation of the inflammatory response and/or promoting tissue vascularization and matrix deposition. This activity arises from the ability of ADRCs to secrete proangiogenic factors and/or differentiate toward lineage-committed cells.…”

“…13 We have also reported that seeding ADRCs onto a widely used collagen dermal substitute increases wound angiogenesis, blood vessel maturation, and matrix remodeling. 11 On the basis of studies such as these, the use of ADRCs as an adjunct to healing has advanced worldwide into multiple clinical trials (e.g., NCT0232696, 14,15 NCT01813279, 16 and NCT02052427 17 ). Small case series applying this approach in chronic cutaneous wounds have similarly been published.…”