Under pressure—how to assess blood pressure in rodents: tail-cuff?

Luther, James M.; Fogo, Agnes B.

doi:10.1016/j.kint.2018.12.034

Cited by 6 publications

(8 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As is also the case in experimental models of diabetes, strain differences have an impact in murine models of hypertension 32 . Although the gold standard method to measure blood pressure in mice is telemetry, controversy exists and the tail-cuff method, which we report here, provides valuable information within normal to high-normal values of blood pressure 33,34 . Previous studies have www.nature.com/scientificreports www.nature.com/scientificreports/ reported hypertension in 129/Sv after 5/6 Nx, both by plethysmography with tail cuff 35 and by telemetry 17 , contrasting with the C57BL/6JRj strain in which blood pressure most frequently does not increase after 5/6 Nx 21 .…”

Section: Compared 5/6mentioning

confidence: 94%

5/6 nephrectomy induces different renal, cardiac and vascular consequences in 129/Sv and C57BL/6JRj mice

Hamzaoui

Djerada

Brunel

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Experimental models of cardiovascular diseases largely depend on the genetic background. Subtotal 5/6 nephrectomy (5/6 Nx) is the most frequently used model of chronic kidney disease (CKD) in rodents. However, in mice, cardiovascular consequences of 5/6 Nx are rarely reported in details and comparative results between strains are scarce. The present study detailed and compared the outcomes of 5/6 Nx in the 2 main strains of mice used in cardiovascular and kidney research, 129/Sv and C57BL/6JRj. Twelve weeks after 5/6 Nx, CKD was demonstrated by a significant increase in plasma creatinine in both 129/ Sv and C57BL/6JRj male mice. Polyuria and kidney histological lesions were more pronounced in 129/Sv than in C57BL/6JRj mice. Increase in albuminuria was significant in 129/Sv but not in C57BL/6JRj mice. Both strains exhibited an increase in systolic blood pressure after 8 weeks associated with decreases in cardiac systolic and diastolic function. Heart weight increased significantly only in 129/Sv mice. Endothelium-dependent mesenteric artery relaxation to acetylcholine was altered after 5/6 Nx in C57BL/6JRj mice. Marked reduction of endothelium-dependent vasodilation to increased intraluminal flow was demonstrated in both strains after 5/6 Nx. Cardiovascular and kidney consequences of 5/6 Nx were more pronounced in 129/Sv than in C57BL/6JRj mice. Chronic kidney disease (CKD) is a major health problem, with a worldwide prevalence of 13% 1. A strong association has been demonstrated between the decrease in glomerular filtration rate (GFR), the risk of developing functional and structural disorders of the heart and cardiovascular (CV) events 2-4. Accordingly, CV disease represents the leading cause of mortality in this population. Multiple mechanisms lead to the CV consequences of CKD, and endothelial dysfunction is considered as a cornerstone in this setting 5,6. Due to limited pathophysiological understanding, a large amount of research is currently dedicated to the deleterious reciprocal interactions of heart and kidney diseases, within the 5 types of cardiorenal syndrome 7. Different models of CKD have been developed in rodents, including kidney mass reduction by 5/6 nephrectomy (5/6 Nx), DOCA salt nephropathy, unilateral ureteral obstruction, oxalate-induced CKD, adenine-induced CKD and genetic mutation of Col4A3 8-12 , but these models do not fully reproduce the CV consequences of CKD observed in humans, including coronary heart disease. 5/6 Nx has been widely used in rats 13,14 because of the persistent decrease in GFR, proteinuria, glomerular sclerosis and hypertension induced, contributing to the major interest of this model 15. To date, several factors, including surgical difficulties, have limited the use of 5/6 Nx in mice 16. This is of critical importance in particular because transgenic mice, most commonly developed on a C57BL/6JRj background, currently represent a key experimental tool. In the literature reporting results of 5/6 Nx in mice, strain-dependent differences appear regarding hypertension, alb...

show abstract

Section: Compared 5/6mentioning

confidence: 94%

5/6 nephrectomy induces different renal, cardiac and vascular consequences in 129/Sv and C57BL/6JRj mice

Hamzaoui

Djerada

Brunel

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…To study the role of vascular EP4 in BP regulation, we generated 2 potentially novel genetically manipulated mouse lines in whom endogenous EP4 expression was conditionally deleted (EC-EP4 -/-) or a full-length human EP4 was specifically overexpressed (EC-hEP4 OE ) in ECs. By using tail-cuff, carotid catheter, and telemetry techniques (34,35), we found that EC deletion of EP4 led to hypertension, while EC overexpression of human EP4 resulted in hypotension, suggesting an essential role of EC EP4 in BP homeostasis. Because both high-salt diet feeding and chronic AngII infusion resulted in a parallel change in BP in control and the EC-EP4 -/or EC-hEP4 OE mice, endothelial EP4 may not contribute to salt and AngII sensitivity, which is in sharp contrast to the findings in mice with VSMC-specific EP4 deletion (VSMC-EP4 -/-) (11).…”

Section: Discussionmentioning

confidence: 95%

Endothelial cell prostaglandin E2 receptor EP4 is essential for blood pressure homeostasis

Fang

et al. 2020

JCI Insight

View full text Add to dashboard Cite

“…Telemetry significantly increases mouse mortality and morbidity ( 62 ) and requires accurate surgery from skilled staff. Tail-cuff measurements may cause animal stress ( 62 , 63 ) and may underestimate blood pressure changes that are due to handling and restraints. However, comparison of tail-cuff and telemetry on rested mice showed that both methods produce similar results ( 64 ).…”

Section: Discussionmentioning

confidence: 99%

“…There is an ongoing debate between telemetry versus tail-cuff measurements. Telemetry significantly increases mouse mortality and morbidity ( 62 ) and requires accurate surgery from skilled staff. Tail-cuff measurements may cause animal stress ( 62 , 63 ) and may underestimate blood pressure changes that are due to handling and restraints.…”

Section: Discussionmentioning

confidence: 99%

HIF1 inhibitor acriflavine rescues early-onset preeclampsia phenotype in mice lacking placental prolyl hydroxylase domain protein 2

Sallais¹,

Park²,

Alahari³

et al. 2022

JCI Insight

View full text Add to dashboard Cite

Preeclampsia is a serious pregnancy disorder that lacks effective treatments other than delivery.Improper sensing of oxygen changes during placentation by prolyl hydroxylases (PHD), specifically PHD2, causes placental Hypoxia-Inducible Factor-1 (HIF1) buildup and abnormal downstream signaling in early-onset preeclampsia; yet therapeutic targeting of HIF1 has never been attempted. Here we generated a conditional (placenta-specific) knockout of Phd2 in mice (Phd2 -/-cKO) to reproduce HIF1 excess and to assess anti-HIF therapy. Conditional deletion of Phd2 in the junctional zone (JZ) during pregnancy increased placental HIF1 content, resulting in abnormal placentation, impaired remodeling of the uterine spiral arteries, and fetal growth restriction. Pregnant dams developed newonset hypertension at mid-gestation (E9.5) in addition to proteinuria and renal and cardiac pathology, hallmarks of severe preeclampsia in humans. Daily injection of acriflavine, a small-molecule inhibitor of HIF1, to pregnant Phd2 -/-cKO mice from E7.5 (prior to hypertension) or E10.5 (after hypertension has been established) to E14.5 corrected placental dysmorphologies and improved fetal growth.Moreover, it reduced maternal blood pressure and reverted renal and myocardial pathology. Thus, therapeutic targeting of the HIF pathway may improve placental development and function, as well as maternal and fetal health, in preeclampsia.

show abstract

Under pressure—how to assess blood pressure in rodents: tail-cuff?

Cited by 6 publications

References 14 publications

5/6 nephrectomy induces different renal, cardiac and vascular consequences in 129/Sv and C57BL/6JRj mice

5/6 nephrectomy induces different renal, cardiac and vascular consequences in 129/Sv and C57BL/6JRj mice

Endothelial cell prostaglandin E2 receptor EP4 is essential for blood pressure homeostasis

HIF1 inhibitor acriflavine rescues early-onset preeclampsia phenotype in mice lacking placental prolyl hydroxylase domain protein 2

Contact Info

Product

Resources

About