Underlying mechanisms involved in the decrease of milk secretion during Escherichia coli endotoxin induced mastitis in lactating mice

Kobayashi, Ken; Oyama, Shoko; Uejyo, Takaaki; Kuki, Chinatsu; Rahman, Morshedur; Kumura, Haruto

doi:10.1186/1297-9716-44-119

Cited by 32 publications

(30 citation statements)

References 51 publications

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“…Intriguingly, our data suggest that the concentration of αs1-casein was significantly increased in milk samples of clinical mastitis-suffered goats, indicating that the milk of these goats may be problematic and chances of allergy are higher. However, we found the expression of αs1-casein in the cytoplasm of MECs was decreased after stimulation of LPS in vitro, consistent with a previous study [14] . The severely milk yield loss in goats with clinical mastitis may be the main reason for the higher concentration of αs1-casein in milk of clinical mastitis-affected goats.…”

Section: Discussionsupporting

confidence: 93%

Sağlıklı ve Mastitisli Keçi Sütünde αs1-kazeini Belirleyen Monoklonal Antikor Geliştirilmesi

Pang¹,

Guan²,

Zuo³

et al. 2019

Kafkas Univ Vet Fak Derg

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This study aimed to evaluate the expression level of caseins during mastitis of goats. Whole goat caseins were used as primary antigens for mouse immunization. A monoclonal antibody (mAb) named 5B with high specificity to goat αs1-casein was developed. Further results showed that mAb 5B can successfully be applied to western blot and ELISA. In addition, immunofluorescence analysis using this mAb showed increased milk αs1-casein level in mastitis-affected goats. In conclusion, this study has established an effective tool to evaluate the expression level of αs1-casein during mastitis development.

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Section: Discussionsupporting

confidence: 93%

Sağlıklı ve Mastitisli Keçi Sütünde αs1-kazeini Belirleyen Monoklonal Antikor Geliştirilmesi

Pang¹,

Guan²,

Zuo³

et al. 2019

Kafkas Univ Vet Fak Derg

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“…Bovine miRNA-29s, which are identical with human miRNA-29s, and bovine FTO mRNA, which is highly homologous to human FTO mRNA, may reach the consumer of pasteurized fresh milk via uptake of milk exosomes [ 84 ]. Viral and bacterial infections of dairy cows may further increase miRNA-29 levels [ 189 , 190 , 196 – 201 ] (Fig. 1 ).…”

Section: Discussionmentioning

confidence: 99%

“…Mastitis, the inflammation of mammary glands resulting from bacterial infection, is a common problem in milk production of dairy cattle. Lipopolysaccharide (LPS) injection into murine lactating mammary glands increased the expression of STAT3 [ 196 ], promoting miRNA-29 expression [ 189 , 190 ].…”

Section: Reviewmentioning

confidence: 99%

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Milk: an epigenetic amplifier of FTO-mediated transcription? Implications for Western diseases

Melnik

2015

J Transl Med

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Single-nucleotide polymorphisms within intron 1 of the FTO (fat mass and obesity-associated) gene are associated with enhanced FTO expression, increased body weight, obesity and type 2 diabetes mellitus (T2DM). The N6-methyladenosine (m6A) demethylase FTO plays a pivotal regulatory role for postnatal growth and energy expenditure. The purpose of this review is to provide translational evidence that links milk signaling with FTO-activated transcription of the milk recipient. FTO-dependent demethylation of m6A regulates mRNA splicing required for adipogenesis, increases the stability of mRNAs, and affects microRNA (miRNA) expression and miRNA biosynthesis. FTO senses branched-chain amino acids (BCAAs) and activates the nutrient sensitive kinase mechanistic target of rapamycin complex 1 (mTORC1), which plays a key role in translation. Milk provides abundant BCAAs and glutamine, critical components increasing FTO expression. CpG hypomethylation in the first intron of FTO has recently been associated with T2DM. CpG methylation is generally associated with gene silencing. In contrast, CpG demethylation generally increases transcription. DNA de novo methylation of CpG sites is facilitated by DNA methyltransferases (DNMT) 3A and 3B, whereas DNA maintenance methylation is controlled by DNMT1. MiRNA-29s target all DNMTs and thus reduce DNA CpG methylation. Cow´s milk provides substantial amounts of exosomal miRNA-29s that reach the systemic circulation and target mRNAs of the milk recipient. Via DNMT suppression, milk exosomal miRNA-29s may reduce the magnitude of FTO methylation, thereby epigenetically increasing FTO expression in the milk consumer. High lactation performance with increased milk yield has recently been associated with excessive miRNA-29 expression of dairy cow mammary epithelial cells (DCMECs). Notably, the galactopoietic hormone prolactin upregulates the transcription factor STAT3, which induces miRNA-29 expression. In a retrovirus-like manner milk exosomes may transfer DCMEC-derived miRNA-29s and bovine FTO mRNA to the milk consumer amplifying FTO expression. There is compelling evidence that obesity, T2DM, prostate and breast cancer, and neurodegenerative diseases are all associated with increased FTO expression. Maximization of lactation performance by veterinary medicine with enhanced miRNA-29s and FTO expression associated with increased exosomal miRNA-29 and FTO mRNA transfer to the milk consumer may represent key epigenetic mechanisms promoting FTO/mTORC1-mediated diseases of civilization.

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“…Trygliceride forms small cytoplasmic lipid droplets (CLD). Furthermore, milk components transferred to the apical membrane of mammary alveolar epithelial cells and secreted into alveolar lumen via specific pathways [10].…”

Section: Introductionmentioning

confidence: 99%

Ameliorative Effect of Infused Watercress on Rat Galactopoiesis following Maternal Separation

2018

J.Trop.Life.Science

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Galactopoiesis is the maintenance of milk production. The process that regulated primarily by prolactin hormone. The research was conducted to evaluate galactopoiesis activity by measuring prolactin level and milk production continuity of lactating rats after oral administration of infused watercress. Twenty lactating rats were randomly divided into five groups (two control groups and three variations of infused watercress dose groups). Serum prolactin was measured using enzyme-linked immunosorbent assay. Milk yield was measured using indirect milk measurement method by Sampson and Jansen. Milk protein level was measured using Kjedahl method. The treatment of infused watercress (10 g/kg body weight), started from postnatal day 3 until 12, indicated ameliorative effect on rat galactopoiesis by increasing milk yield and prolactin levels, but not milk total protein.

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Underlying mechanisms involved in the decrease of milk secretion during Escherichia coli endotoxin induced mastitis in lactating mice

Cited by 32 publications

References 51 publications

Sağlıklı ve Mastitisli Keçi Sütünde αs1-kazeini Belirleyen Monoklonal Antikor Geliştirilmesi

Sağlıklı ve Mastitisli Keçi Sütünde αs1-kazeini Belirleyen Monoklonal Antikor Geliştirilmesi

Milk: an epigenetic amplifier of FTO-mediated transcription? Implications for Western diseases

Ameliorative Effect of Infused Watercress on Rat Galactopoiesis following Maternal Separation

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