Understanding and engineering alcohol-tolerant bacteria using OMICS technology

Horinouchi, Takaaki; Maeda, Tomoya; Furusawa, Chikara

doi:10.1007/s11274-018-2542-4

Cited by 38 publications

(26 citation statements)

References 93 publications

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“…Finally, adaptive laboratory evolution (ALE) can help improve the tolerance of microbes to butanol. ALE is a powerful technique in which a microorganism is cultivated continuously for several generations, improving its fitness in a response to a certain condition (selective pressure) by natural selection (Horinouchi, Maeda, & Furusawa, 2018). The subsequent whole-genome sequencing of the adapted strains can identify the mutations associated with butanol's tolerance.…”

Section: Improvement Of E Coli Tolerance To Butanolmentioning

confidence: 99%

“…The subsequent whole-genome sequencing of the adapted strains can identify the mutations associated with butanol's tolerance. Further integration of "omics" technology can help to unravel the regulatory and metabolic mechanisms associated with butanol tolerance (Horinouchi et al, 2018). Jeong et al (2017) have evolved a E. coli strain able to tolerate 1.3% (v/v) butanol.…”

Section: Improvement Of E Coli Tolerance To Butanolmentioning

confidence: 99%

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Metabolic engineering strategies for butanol production in Escherichia coli

Ferreira

Pereira

Wahl

et al. 2020

Biotech & Bioengineering

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The global market of butanol is increasing due to its growing applications as solvent, flavoring agent, and chemical precursor of several other compounds. Recently, the superior properties of n-butanol as a biofuel over ethanol have stimulated even more interest. (Bio)butanol is natively produced together with ethanol and acetone by Clostridium species through acetone-butanol-ethanol fermentation, at noncompetitive, low titers compared to petrochemical production. Different butanol production pathways have been expressed in Escherichia coli, a more accessible host compared to Clostridium species, to improve butanol titers and rates. The bioproduction of butanol is here reviewed from a historical and theoretical perspective. All tested rational metabolic engineering strategies in E. coli to increase butanol titers are reviewed: manipulation of central carbon metabolism, elimination of competing pathways, cofactor balancing, development of new pathways, expression of homologous enzymes, consumption of different substrates, and molecular biology strategies. The progress in the field of metabolic modeling and pathway generation algorithms and their potential application to butanol production are also summarized here. The main goals are to gather all the strategies, evaluate the respective progress obtained, identify, and exploit the outstanding challenges.

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Section: Improvement Of E Coli Tolerance To Butanolmentioning

confidence: 99%

Section: Improvement Of E Coli Tolerance To Butanolmentioning

confidence: 99%

Metabolic engineering strategies for butanol production in Escherichia coli

Ferreira

Pereira

Wahl

et al. 2020

Biotech & Bioengineering

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“…Escherichia coli expresses 304 TFs (Perez-Rueda et al, 2015), though only a few have been reported to regulate butanol tolerance-related genes (Reyes et al, 2012;Horinouchi et al, 2018). Furthermore, the corresponding genes regulated by these TFs and the associated regulatory mechanism have not yet been clarified (Aquino et al, 2017), limiting the improvement of butanol-tolerant chassis strains using a rational-design engineering strategy.…”

Section: Introductionmentioning

confidence: 99%

A Novel Butanol Tolerance-Promoting Function of the Transcription Factor Rob in Escherichia coli

Wang

Xue

et al. 2020

Front. Bioeng. Biotechnol.

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Producing high concentrations of biobutanol is challenging, primarily because of the toxicity of butanol toward cells. In our previous study, several butanol tolerancepromoting genes were identified from butanol-tolerant Escherichia coli mutants and inactivation of the transcriptional regulator factor Rob was shown to improve butanol tolerance. Here, the butanol tolerance characteristics and mechanism regulated by inactivated Rob are investigated. Comparative transcriptome analysis of strain DTrob, with a truncated rob in the genome, and the control BW25113 revealed 285 differentially expressed genes (DEGs) to be associated with butanol tolerance and categorized as having transport, localization, and oxidoreductase activities. Expression of 25 DEGs representing different functional categories was analyzed by quantitative reverse transcription PCR (qRT-PCR) to assess the reliability of the RNA-Seq data, and 92% of the genes showed the same expression trend. Based on functional complementation experiments of key DEGs, deletions of glgS and yibT increased the butanol tolerance of E. coli, whereas overexpression of fadB resulted in increased cell density and a slight increase in butanol tolerance. A metabolic network analysis of these DEGs revealed that six genes (fadA, fadB, fadD, fadL, poxB, and acs) associated with acetyl-CoA production were significantly upregulated in DTrob, suggesting that Rob inactivation might enhance butanol tolerance by increasing acetyl-CoA. Interestingly, DTrob produced more acetate in response to butanol stress than the wild-type strain, resulting in the upregulation expression of some genes involved in acetate metabolism. Altogether, the results of this study reveal the mechanism underlying increased butanol tolerance in E. coli regulated by Rob inactivation.

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“…[30] Some stand-alone SARPs (e.g.,t he large SARP AfsR) globally control secondary metabolism and morphological development. [31,32] Thus SARP gene activation can influence the productiono fm ultiple secondary metabolites.…”

Section: Introductionmentioning

confidence: 99%

Production of a Novel Amide‐Containing Polyene by Activating a Cryptic Biosynthetic Gene Cluster in Streptomyces sp. MSC090213JE08

Katsuyama

Onaka

et al. 2016

ChemBioChem

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Streptomyces sp. MSC090213JE08 seems to have more than 20 cryptic biosynthetic gene clusters for secondary metabolites. We aimed to activate some of them by forced production of Streptomyces antibiotic regulatory protein (SARP) family transcriptional activators. We constructed seven recombinant strains, each of which contained a SARP gene under the control of a constitutive promoter, and subjected them to comparative metabolic profiling analysis. Four of the seven strains produced nine metabolites that were hardly detected in the control strains. We isolated a new metabolite (named ishigamide) from the SARP-7-expressing strain and determined its structure as 3-((2E,4E,6E,8E)-13-hydroxytetradeca-2,4,6,8-tetraenamido)propanoic acid. Genome scanning and gene disruption studies identified the ishigamide biosynthetic gene cluster adjacent to the SARP-7 gene. We think that a new subfamily of type II polyketide synthase is involved in the biosynthesis of the polyene structure of ishigamide.

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Understanding and engineering alcohol-tolerant bacteria using OMICS technology

Cited by 38 publications

References 93 publications

Metabolic engineering strategies for butanol production in Escherichia coli

Metabolic engineering strategies for butanol production in Escherichia coli

A Novel Butanol Tolerance-Promoting Function of the Transcription Factor Rob in Escherichia coli

Production of a Novel Amide‐Containing Polyene by Activating a Cryptic Biosynthetic Gene Cluster in Streptomyces sp. MSC090213JE08

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