Understanding and Engineering the Pulmonary Vasculature

Ng, Wai Hoe; Varghese, Barbie; Ren, Xi

doi:10.1007/978-3-031-26625-6_12

Cited by 3 publications

(1 citation statement)

References 114 publications

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“…The pulmonary bed differs from other vascular beds in many ways as previously discussed 12 : The physical organization of the pulmonary vascular bed and its endothelial surface differs from other beds 38,39 . Moreover, the lungs differ in the direction of O 2 and CO 2 exchange, and in pH shift.…”

Section: Discussionmentioning

confidence: 97%

Studies of infused megakaryocytes into mice support a “catch-and-release” model of pulmonary-centric thrombopoiesis

Kim,

Jarocha,

Johnson

et al. 2024

Preprint

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Many aspects of thrombopoiesis, the release of platelets from megakaryocytes (Mks), remain under debate, including where this process occurs. Murine lungin situ-microscopy studies suggested that a significant fraction of circulating platelets were released from lung-entrapped, marrow-derived Mks. We now confirm thesein situstudies that endogenous mMks are entrapped in the lungs and show that intravenously infusedin vitro-differentiated, mature murine (m) and human (h) Mks are similarly entrapped followed by shedding of their cytoplasm over ∼30 minutes with a peak number of released platelets occurring 1.5-4 hours later. However, while infused Mks from both species shed large intrapulmonary cytoplasmic fragments that underwent further processing into platelet-sized fragments, the two differed: many mMks escaped from and then recycled back to the lungs, while most hMks were enucleated upon first intrapulmonary passage. Infused immature hMks, inflammatory hMks, umbilical cord-blood-derived hMks and immortalized Mk progenitor cell (imMKCL)-derived hMks were also entrapped in the lung of recipient mice, and released their cytoplasm, but did so to different degrees. Intraarterial infused hMks resulted in few Mks being entrapped in tissues other than the lungs and was accompanied by a blunted and delayed rise in circulating human platelets. These studies demonstrate that the lung entraps and processes both circulating Mks and released large cytoplasmic fragments consistent with a recent lung/heart murine study and support a pulmonary-centric “catch-and-release” model of thrombopoiesis. Thus, thrombopoiesis is a drawn-out process with the majority of cytoplasmic processing derived from Mks occurring in the pulmonary bed.Key PointsInfusedin vitro-differentiated megakaryocytes synchronously release cytoplasmic fragments highly selectively in the pulmonary bed.Large, released megakaryocyte fragments recycle to the lungs, undergo further fission, terminally form platelets.

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Section: Discussionmentioning

confidence: 97%

Studies of infused megakaryocytes into mice support a “catch-and-release” model of pulmonary-centric thrombopoiesis

Kim,

Jarocha,

Johnson

et al. 2024

Preprint

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Blood flow‐induced angiocrine signals promote organ growth and regeneration

Follert,

Große‐Segerath,

Lammert

2024

BioEssays

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Recently, we identified myeloid‐derived growth factor (MYDGF) as a blood flow‐induced angiocrine signal that promotes human and mouse hepatocyte proliferation and survival. Here, we review literature reporting changes in blood flow after partial organ resection in the liver, lung, and kidney, and we describe the angiocrine signals released by endothelial cells (ECs) upon blood flow alterations in these organs. While hepatocyte growth factor (HGF) and MYDGF are important angiocrine signals for liver regeneration, by now, angiocrine signals have also been reported to stimulate hyperplasia and/or hypertrophy during the regeneration of lungs and kidneys. In addition, angiocrine signals play a critical role in tumor growth. Understanding the mechano‐elastic properties and flow‐mediated alterations in the organ‐specific microvasculature is crucial for therapeutic approaches to maintain organ health and initiate organ renewal.

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Decellularized extracellular matrix for organoid and engineered organ culture

Guo,

Liu,

Zhang

et al. 2024

J Tissue Eng

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The repair and regeneration of tissues and organs using engineered biomaterials has attracted great interest in tissue engineering and regenerative medicine. Recent advances in organoids and engineered organs technologies have enabled scientists to generate 3D tissue that recapitulate the structural and functional characteristics of native organs, opening up new avenues in regenerative medicine. The matrix is one of the most important aspects for improving organoids and engineered organs construction. However, the clinical application of these techniques remained a big challenge because current commercial matrix does not represent the complexity of native microenvironment, thereby limiting the optimal regenerative capacity. Decellularized extracellular matrix (dECM) is expected to maintain key native matrix biomolecules and is believed to hold enormous potential for regenerative medicine applications. Thus, it is worth investigating whether the dECM can be used as matrix for improving organoid and engineered organs construction. In this review, the characteristics of dECM and its preparation method were summarized. In addition, the present review highlights the applications of dECM in the fabrication of organoids and engineered organs.

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Understanding and Engineering the Pulmonary Vasculature

Cited by 3 publications

References 114 publications

Studies of infused megakaryocytes into mice support a “catch-and-release” model of pulmonary-centric thrombopoiesis

Studies of infused megakaryocytes into mice support a “catch-and-release” model of pulmonary-centric thrombopoiesis

Blood flow‐induced angiocrine signals promote organ growth and regeneration

Decellularized extracellular matrix for organoid and engineered organ culture

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