Understanding Chilling Tolerance Traits Using Arabidopsis Chilling-Sensitive Mutants

Zoldan, Dana; Band, R. Shekaste; Guy, Charles L.; Porat, Ron

doi:10.1007/978-1-4614-0815-4_7

Cited by 8 publications

(2 citation statements)

References 39 publications

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“…Many cellular membrane systems are damaged in response to chilling (2, 13), and changes in membrane lipid compositions are required to achieve chilling tolerance (7, 30). We profiled membrane lipids from sorghum, Urochloa, and foxtail millet seedlings grown under control and chilling-stress conditions.…”

Section: Resultsmentioning

confidence: 99%

“…At the cellular level, low-temperature stress leads to increased membrane rigidity and impaired containment of cytosolic contents, resulting in cell death (7, 8). Changes in glycerolipids, major components of cell membranes, include membrane lipid polyunsaturation (9, 10), changing the ratio of lipid head groups, and removing membrane-destabilizing lipids in response to low temperature (11, 12).…”

Section: Introductionmentioning

confidence: 99%

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Rhythmic lipid and gene expression responses to chilling in panicoid grasses

Raju,

Zhang,

Mahboub

et al. 2023

Preprint

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Chilling stress threatens plant growth and development, particularly affecting membrane fluidity and cellular integrity. Understanding plant membrane responses to chilling stress is important for unraveling the molecular mechanisms of stress tolerance. Whereas core transcriptional responses to chilling stress and stress tolerance are conserved across species, the associated changes in membrane lipids appear to be less conserved, as which lipids are affected by chilling stress varies by species. Here, we investigated changes in gene expression and membrane lipids in response to chilling stress during one diurnal cycle in sorghum (Sorghum bicolor), Urochloa (browntop signal grass,Urochloa fusca) (lipids only), and foxtail millet (Setaria italica), leveraging their evolutionary relatedness and differing levels of chilling-stress tolerance. We show that most chilling-induced lipid changes are conserved across the three species, while we observed distinct, time-specific responses in chilling-tolerant foxtail millet, indicating the presence of a finely orchestrated adaptive mechanism. We detected diurnal rhythmicity in lipid responses to chilling stress in the three grasses, which were also present in Arabidopsis (Arabidopsis thaliana), suggesting the conservation of rhythmic patterns across species and highlighting the importance of accounting for diurnal effects. When integrating lipid datasets with gene expression profiles, we identified potential candidate genes that showed corresponding transcriptional changes in response to chilling stress, providing insights into the differences in regulatory mechanisms between chilling-sensitive sorghum and chilling-tolerant foxtail millet.Significance StatementPlants respond to low-temperature stress in myriad ways. While core transcriptional changes are conserved across species, specific adaptive strategies do exist. However, membrane lipid responses during chilling do not appear to be conserved. Here, we collected samples from control and chilling stress–treated seedlings [PSC4] to assess gene expression and membrane lipids in three panicoid grasses to show that lipid metabolic changes follow a daily rhythm. Lipid changes in chilling-tolerant foxtail millet occurred at specific time points, partly explaining the difficulty in finding conserved chilling-induced lipid changes in previous reports. We identified specific orthologs in sorghum and foxtail millet that showed a correlation between gene expression and lipid metabolic changes; these orthologs may be used as potential target genes for developing chilling-tolerant sorghum varieties.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rhythmic lipid and gene expression responses to chilling in panicoid grasses

Raju,

Zhang,

Mahboub

et al. 2023

Preprint

View full text Add to dashboard Cite

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Phytomicrobiome Community: An Agrarian Perspective Towards Resilient Agriculture

Sharma

Kapoor

et al. 2021

Plant Performance Under Environmental Stress

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CsWRKY29, a key transcription factor in tea plant for freezing tolerance, ABA sensitivity, and sugar metabolism

Xue,

Huang,

Zhao

2024

Sci Rep

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Tea plants ( Camellia sinensis L.) are prone to spring frosts, leading to substantial economic damage. WRKY transcription factors are key in plant abiotic stress responses, yet the role of CsWRKY29 in freezing tolerance is unclear. In this study, quantitative real-time PCR (qRT-PCR) and transient green fluorescent protein assay revealed that CsWRKY29 localizes to the nucleus and its expression is induced by cold and abscisic acid (ABA). CsWRKY29 overexpression in Arabidopsis enhanced freezing tolerance, reduced electrolyte leakage, increased soluble sugars, and boosted superoxide dismutase activity, with upregulated COR genes. These lines also showed heightened ABA and glucose sensitivity. Cold treatment of CsWRKY29 -overexpressing lines upregulated AtABI5 , AtHXK1 , and AtSUS4 compared to wild type, and yeast one-hybrid assays confirmed CsWRKY29 binding to the W-box in the CsABI5 promoter. Furthermore, the application of virus-induced gene silencing (VIGS) technology to reduce CsWRKY29 expression in tea plants revealed a significant decrease in the transcript levels of CsCBFs , CsABI5 , CsHXK1 , and CsSUS4 in the silenced plants. In summary, our findings indicate that CsWRKY29 may serve as a critical transcription factor that contributes to freezing tolerance, ABA responsiveness, and sugar metabolism within tea plants.

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Understanding Chilling Tolerance Traits Using Arabidopsis Chilling-Sensitive Mutants

Cited by 8 publications

References 39 publications

Rhythmic lipid and gene expression responses to chilling in panicoid grasses

Rhythmic lipid and gene expression responses to chilling in panicoid grasses

Phytomicrobiome Community: An Agrarian Perspective Towards Resilient Agriculture

CsWRKY29, a key transcription factor in tea plant for freezing tolerance, ABA sensitivity, and sugar metabolism

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