Understanding Dopamine Receptor Mediated Regulation of Insulin Secretion by Two-Color Spatial Intensity Distribution Analysis

Abstract

this, we systematically investigate how different initial distributions of bacterial populations influence the subsequent efficiency of collective long-range signal propagation in the population. We compare our results with known experimental data, and discuss limitations and extensions to our modeling framework. 715-Pos Board B495 Bcl-2 OR Bcl-xL Overexpression Stimulates both Oxidative and Fermentative Components of Carbohydrate Metabolism

Spatial Intensity Distribution Analysis: Studies of G Protein-Coupled Receptor Oligomerisation

“…Such studies have provided new insights into this contentious issue, but many challenges remain (see Outstanding Questions); both in terms of further development of SpIDA, and how information from such studies can be combined with other imaging modalities and/or with approaches, including molecular dynamics simulations of ligand interactions with GPCRs. Given the significant interest in the potential existence of heteromeric GPCR complexes, the development of two-colour SpIDA has recently been described by Godin and co-workers [42] , and this is likely to be useful in addressing proportions of GPCR homomers and heteromers present in a sample that coexpresses a compatible pair of GPCRs. Importantly, because SpIDA is based on analysis of simple confocal images, and these can be derived from fixed cells and tissues, it should be possible to perform SpIDA measurements more routinely on tissue sections from mice and other model organisms expressing mEGFP, or other wavelength-shifted, fluorophore-tagged GPCRs of interest.…”

“…To date, SpIDA has only been used to observe and quantify GPCR homo-oligomers. However, two-colour SpIDA [42] , using pairs of fluorescent proteins with similar excitation but different emission wavelengths, is being developed and is likely to allow concurrent detection and quantification of homo- and hetero-oligomeric interactions. Can SpIDA Be Used to Monitor Receptor Quaternary Organisation in Native Tissues?…”