Understanding Factors that Limit Enzymatic Hydrolysis of Biomass

Laureano-Pérez, Lizbeth; Teymouri, Farzaneh; Alizadeh, H.; Dale, Bruce E.

doi:10.1007/978-1-59259-991-2_91

Cited by 101 publications

(94 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cork tertiary wall (outer layer) is essentially enriched in polysaccharides [16]. On the contrary, in wood this layer contains also lignin [15], which hampers the degradation of the polysaccharides [64]. Higher polysaccharide accessibility might thus partially explain the observed increase of -glucosidase (AN2828) in the cork secretome.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…Lignin is a branched polyaromatic polymer constituted of variable proportions of phenylpropanoid units [15] predominantly linked by β-O-4-linked ether bonds that are not easily hydrolysable. This polymer is known to impact on the cellulose digestibility mainly due to their cross-linking in the cell wall [64]. In cork cell walls, lignin is especially abundant in the primary wall, while the tertiary wall (outer cell wall layer) is essentially composed by polysaccharides [16].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Investigating Aspergillus nidulans secretome during colonisation of cork cell walls

Martins

Garcia

Varela

et al. 2014

Journal of Proteomics

View full text Add to dashboard Cite

Cork, the outer bark of Quercus suber, shows a unique compositional structure, a set of remarkable properties, including high recalcitrance. Cork colonisation by Ascomycota remains largely overlooked. Herein, Aspergillus nidulans secretome on cork was analysed (2DE).Proteomic data were further complemented by microscopic (SEM) and spectroscopic (ATR-FTIR) evaluation of the colonised substrate and by targeted analysis of lignin degradation compounds (UPLC-HRMS). Data showed that the fungus formed an intricate network of hyphae around the cork cell walls, which enabled polysaccharides and lignin superficial degradation, but probably not of suberin. The degradation of polysaccharides was suggested by the identification of few polysaccharide degrading enzymes (β-glucosidases and endo-1,5--L-arabinosidase).Lignin degradation, which likely evolved throughout a Fenton-like mechanism relying on the activity of alcohol oxidases, was supported by the identification of small aromatic compounds (e.g. cinnamic acid and veratrylaldehyde) and of several putative high molecular weight lignin degradation products. In addition, cork recalcitrance was corroborated by the identification of several protein species which are associated with autolysis. Finally, stringent comparative proteomics revealed that A. nidulans colonisation of cork and wood share a common set of enzymatic mechanisms. However the higher polysaccharide accessibility in cork might explain the increase of β-glucosidase in cork secretome.

show abstract

Section: Accepted M Manuscriptmentioning

confidence: 99%

mentioning

confidence: 99%

Investigating Aspergillus nidulans secretome during colonisation of cork cell walls

Martins

Garcia

Varela

et al. 2014

Journal of Proteomics

View full text Add to dashboard Cite

show abstract

“…It has been reported that a decrease in cellulose crystallinity especially influences the initial rate of cellulose hydrolysis by cellulase. 5) Physical or chemical pretreatment to disrupt the crystalline structure of cellulose is often used to promote the hydrolysis of biomass. In addition to cellulose crystallinity, lignin affects the enzymatic hydrolysis of lignocellulosic biomass because it forms a physical barrier to attack by enzymes.…”

mentioning

confidence: 99%

Effects of Cellulose Crystallinity, Hemicellulose, and Lignin on the Enzymatic Hydrolysis ofMiscanthus sinensisto Monosaccharides

Yoshida

Liu

Uchida

et al. 2008

Bioscience, Biotechnology, and Biochemistry

329

174

View full text Add to dashboard Cite

“…This result is consistent with the composition analysis and SEM observation. After pretreatment, the transmittance of bands, like 3,412 cm -1 (H-bonded O-H stretching) and 990 cm -1 (C-H deformation) have some increase because the content of these groups in substrate increased, reflecting the depolymerization of lignocellulose polymers and disruption of the crystalline structure [28][29].…”

Section: Resultsmentioning

confidence: 99%

NaOH Pretreatment of Wheat Straw at a Mesophilic Temperature: Effect on Hydrolysis and Loss of Organic Carbon

Jiang¹,

Zeng²,

Nie³

et al. 2016

Pol. J. Environ. Stud.

View full text Add to dashboard Cite

Understanding Factors that Limit Enzymatic Hydrolysis of Biomass

Cited by 101 publications

References 21 publications

Investigating Aspergillus nidulans secretome during colonisation of cork cell walls

Investigating Aspergillus nidulans secretome during colonisation of cork cell walls

Effects of Cellulose Crystallinity, Hemicellulose, and Lignin on the Enzymatic Hydrolysis ofMiscanthus sinensisto Monosaccharides

NaOH Pretreatment of Wheat Straw at a Mesophilic Temperature: Effect on Hydrolysis and Loss of Organic Carbon

Contact Info

Product

Resources

About