After four decades of HIV/AIDS pandemic, African Americans represent at least 43 percent of infections but only makeup 12.6 percent of the USA population (Census Bureau, 2010). It is apparent from these rates that additional information is needed to further understand the possible linkages between HIV/AIDS infection and members of this ethnic group. This exploratory study analyzed relationships between knowledge and attitudes toward safe sex, and risky behaviors on an HBCU. Using purposive sampling, a group of 364 students was surveyed. Factors assessed as determinants of risky sexual behaviors were: (1) general HIV knowledge, (2) attitude towards safe sex practices, and (3) unprotected or unsafe sex practices. Findings suggest that the average count of risk incidents among women was significantly higher than men; however, women are more inclined to have preventive contagion practices for sexually transmitted diseases than men. In addition, they are more aware of what constitutes risky sexual behavior. The findings should assist universities in developing educational programs and culturally sensitive intervention and prevention strategies aimed at students who are at risk for participating in risky behaviors.