Understanding improved osteoblast behavior on select nanoporous anodic alumina

Ni, Siyu; Yan, Xinxin; Ni, Shirong; Chen, Ting; Webster, Thomas J.

doi:10.2147/ijn.s60346

Cited by 23 publications

(15 citation statements)

References 38 publications

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“…In recent years, it has been recognized that biomaterial porosity greatly influences cellular behavior not only at the proliferative but also the differentiation stage (Bignon et al, 2003 ; Karageorgiou and Kaplan, 2005 ; Lew et al, 2012 ). Therefore, it has been suggested that by adapting surface properties to the desired cell behavior, we may open up the possibility of controlling cell behavior, thereby improving implant performance (Ni et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of Porosity of Alumina and Zirconia Ceramics toward Pre-Osteoblast Response

Hadjicharalambous

Prymak

Loza

et al. 2015

Front. Bioeng. Biotechnol.

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It is acknowledged that cellular responses are highly affected by biomaterial porosity. The investigation of this effect is important for the development of implanted biomaterials that integrate with bone tissue. Zirconia and alumina ceramics exhibit outstanding mechanical properties and are among the most popular implant materials used in orthopedics, but few data exist regarding the effect of porosity on cellular responses to these materials. The present study investigates the effect of porosity on the attachment and proliferation of pre-osteoblastic cells on zirconia and alumina. For each composition, ceramics of three different porosities are fabricated by sintering, and characterized using scanning electron microscopy, energy dispersive X-ray spectroscopy and X-ray powder diffraction. Cell proliferation is quantified, and microscopy is employed to qualitatively support the proliferation results and evaluate cell morphology. Cell adhesion and metabolic activity are found comparable among low porosity zirconia and alumina. In contrast, higher porosity favors better cell spreading on zirconia and improves growth, but does not significantly affect cell response on alumina. Between the highest porosity materials, cell response on zirconia is found superior to alumina. Results show that an average pore size of ~150 μm and ~50% porosity can be considered beneficial to cellular growth on zirconia ceramics.

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Section: Discussionmentioning

confidence: 99%

Effect of Porosity of Alumina and Zirconia Ceramics toward Pre-Osteoblast Response

Hadjicharalambous

Prymak

Loza

et al. 2015

Front. Bioeng. Biotechnol.

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“…4 5 AAO can form different types of anodic oxides: a non-porous barrier type, when fabricated using neutral electrolytes, and a porous-type oxide, when fabricated using acidic electrolytes. Nanoporous alumina is a suitable template for the production of nanostructures 6 and has been used in medical applications, e.g., tissue engineering, in recent years based on reports that various types of cells, such as neurons, 7 hepatic cells, 8 smooth muscle cells, 9 keratinocytes, 10 fibroblasts, 11 and osteoblasts, 12 are capable of attaching to its surface. Furthermore, neurite outgrowth is known to be accelerated by pitch-dependent AAO substrates, 7 and hepatic cell growth is accelerated on self-supporting, mechanically stabilized nanoporous aluminum oxide membranes.…”

Section: Introductionmentioning

confidence: 99%

Cell Adhesion and Growth on the Anodized Aluminum Oxide Membrane

Park¹,

Moon²,

Kim³

et al. 2016

j biomed nanotechnol

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Nanotopological cues are popular tools for in vivo investigation of the extracellular matrix (ECM) and cellular microenvironments. The ECM is composed of multiple components and generates a complex microenvironment. The development of accurate in vivo methods for the investigation of ECM are important for disease diagnosis and therapy, as well as for studies on cell behavior. Here, we fabricated anodized aluminum oxide (AAO) membranes using sulfuric and oxalic acid under controlled voltage and temperature. The membranes were designed to possess three different pore and interpore sizes, AAO-1, AAO-2, and AAO-3 membranes, respectively. These membranes were used as tools to investigate nanotopology-signal induced cell behavior. Cancerous cells, specifically, the OVCAR-8 cell-line, were cultured on porous AAO membranes and the effects of these membranes on cell shape, proliferation, and viability were studied. AAO-1 membranes bearing small sized pores were found to maintain the spreading shape of the cultured cells. Cells cultured on AAO-2 and AAO-3 membranes, bearing large pore-sized AAO membranes, changed shape from spreading to rounding. Furthermore, cellular area decreased when cells were cultured on all three AAO membranes that confirmed decreased levels of focal adhesion kinase (FAK). Additionally, OVCAR-8 cells exhibited increased proliferation on AAO membranes possessing various pore sizes, indicating the importance of the nanosurface structure in regulating cell behaviors, such as cell proliferation. Our results suggest that porous-AAO membranes induced nanosurface regulated cell behavior as focal adhesion altered the intracellular organization of the cytoskeleton. Our results may find potential applications as tools in in vivo cancer research studies.

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“…It was reported that enhancing the osteoblast density on the aluminum surface can be achieved by improving the surface roughness only and without any change in chemistry. [68] To study the importance of pore depth, Thakur et al studied the living cells (vascular endothelial cells (ECV 304) response on PAA surface. The cells were cultured on the nanoporous surface and after 1 day treated with polymerized actin in the presence of 3.7% formaldehyde.…”

Section: Cell Attachment On Naa Surfacesmentioning

confidence: 99%

Nanoporous Anodic Alumina Platforms for Drug Delivery Applications: Recent Advances and Perspective

Kapruwan

Ferré‐Borrull

Marsal

2020

Adv Materials Inter

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dosages to maintain drug level in the body and there is usually a rapid release of the drug directly into the blood circulation within a short time before reaching the desirable site, which might possess a risk of damaging healthy organs. [3] The main problem with these types of delivery vehicles includes poor patient compliance, lack of selectivity, unavoidable drug solubility, and biodistribution along with unfavorable elution kinetics. [4] With recent advances in nanofabrication technologies, drug delivery systems have achieved a significant progress in terms of patient safety, bioavailability, and achieving required therapeutic efficiency. [5,6] Two new branches, i.e., localized drug delivery systems (LDDSs) and targeted drug delivery systems (TDDSs) have emerged to efficiently deliver a drug inside the body using many nano-based drug carriers. These carriers include nanoparticles, liposomes, dendrimers, micelles, nanospheres, nanotubes, graphene. [7-11] Both of these concepts have already been executed in clinical practices performing excellently in terms of cost-effectiveness, less painful technologies, controlled and reliable drug release profile. [12] Amidst different categories of nanomaterials developed for drug release, inorganic nanoporous structures are the center of attraction for most of the researchers today due to its ease of fabrication, cost-effectiveness, and ability to tune structural parameters precisely. [13] In recent years, nanoporous anodic alumina (NAA), porous silicon (pSi), and titania were thoroughly investigated among all nanoporous materials specifically for developing new drug-releasing strategies due to their excellent physiochemical properties and biocompatible properties. [14-16] Among all the materials mentioned above, NAA remains one of the best choices due to its excellent chemical inertness, biocompatibility, enhanced mechanical strength, tunable chemistry, and controlled pore dimensions and volumes for loading and releasing drugs in a controlled manner. [14,17-20] In addition to its biomedical importance, NAA is also being utilized in optical biosensing, energy production, and catalytic applications. [13,21-25] This review compiles the most recent advances in NAA platforms for drug delivery applications with a short introduction for efficient drug delivery strategies developed over the years. To start with, the fundamental fabrication process, their properties, and biocompatibility of NAA are briefly introduced. The main intention is to summarize the different nanostructures based on NAA currently being developed for drug delivery applications, their advantages, and limitations. Furthermore, For several decades, nanoporous anodic alumina (NAA) has been prepared through inexpensive electrochemical anodization of aluminum to prepare diversified periodically ordered nanostructures. It acts as an excellent drug reservoir due to its excellent physical and chemical properties hence minimizing the drug loss and increasing bioavailability to the target site. A rapid increase in ex...

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Understanding improved osteoblast behavior on select nanoporous anodic alumina

Cited by 23 publications

References 38 publications

Effect of Porosity of Alumina and Zirconia Ceramics toward Pre-Osteoblast Response

Effect of Porosity of Alumina and Zirconia Ceramics toward Pre-Osteoblast Response

Cell Adhesion and Growth on the Anodized Aluminum Oxide Membrane

Nanoporous Anodic Alumina Platforms for Drug Delivery Applications: Recent Advances and Perspective

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