Understanding neuroendocrine regulatory pathways of sexual behavior in teleosts

Ahamed, Summaiyya Hanum; Tokumoto, Toshinobu

doi:10.1016/j.aaf.2023.04.007

Cited by 3 publications

(1 citation statement)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hormones function as pheromones when they are excreted from the fish’s body, thus giving rise to the phrase "hormonal pheromone" [ 2 ]. Subsequent comprehensive investigations into the processes underlying the actions of hormones and pheromones in diverse fish species have revealed that the mechanisms governing sexual behavior exhibit divergence, with distinct species-specific variations [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

In vivo induction of male sexual behavior in zebrafish by adding agents to water

Ahamed,

Hassan,

Mustary

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Successful fertilization in fish mating occurs when egg maturation in the ovary of the female, ovulation, sperm maturation in the testis of the male, and reproductive behaviors in both sexes are triggered in synchrony. The male sexual behavior of fish is induced by hormones and pheromones. In a previous study, we demonstrated that externally applied hormones added to the water can induce oocyte maturation and ovulation in female zebrafish. Here, we attempted to establish a similar method to induce the sexual behavior of male zebrafish. The male sex steroid testosterone (Tes) triggered sexual behavior within several hours in vivo when administered directly into the surrounding water. A selective agonist for membrane progesterone receptor (mPR), Org OD-02 (Org), also induced sexual behavior. Through trials of various combinations of compounds, we found that the most effective conditions were achieved by treatment with a mixture of testosterone (Tes) and Org. The effect of treatment was evaluated by the number of fertilized eggs obtained by pairing with females with induced ovulation in vivo. The period necessary for the induction of male sexual behavior was evaluated by time course experiments. The success rate of mating and the number of fertilized eggs reached the maximum level at 3–4 hours of treatment. The duration of hormonal treatment was confirmed by counting the number of hooking occurrences, which is the final cue to induce spawning by females. In summary, we have established a method to induce male sexual behavior in zebrafish in vivo. The method can be used to obtain fertilized eggs in zebrafish by simply adding agents into the water.

show abstract

Section: Introductionmentioning

confidence: 99%