Understanding of the role of dilution on evaporative deposition patterns of blood droplets over hydrophilic and hydrophobic substrates

Iqbal, Razia; Shen, Amy Q.; Sen, A. K.

doi:10.1016/j.jcis.2020.04.109

Cited by 34 publications

(31 citation statements)

References 38 publications

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“…The large peak that was noticed at of (v/v) [Figs. 2 (I) and S1 - S3 (I), the presence of a similar peak has also been reported in 20 , may have some correlation to this viscous behavior during the drying process. However, the experiments reported in 20 did not concentrate on diverse ranges of dilution; and hence the study failed to observe the transition at .…”

Section: Discussionsupporting

confidence: 79%

“…These results appear consistent with the DI water dilution and so minimizes this mechanism. However, it is worth mentioning that the dilution of the blood by PBS (at its fixed concentration) will increase the appearance of salts residues or crystals 20 , which influences the statistics derived from the optical images, and lead to artifacts in the scanning electron microscopy images.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, diluted blood samples (into different concentration ranges) have shown insightful drying patterns in blood pattern analysis (BPA) 16 – 19 . Sen et al 20 , in a different study, examined the effects of diluted blood droplets at a fixed saline concentration by changing the substrate conditions. The study concluded that the transition of the cracking to the non-cracking regime exists for the droplets deposited on the hydrophilic substrate.…”

Section: Introductionmentioning

confidence: 99%

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Concentration-driven phase transition and self-assembly in drying droplets of diluting whole blood

Pal

Gope

Obayemi

et al. 2020

Sci Rep

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Multi-colloidal systems exhibit a variety of structural and functional complexity owing to their ability to interact amongst different components into self-assembled structures. This paper presents experimental confirmations that reveal an interesting sharp phase transition during the drying state and in the dried film as a function of diluting concentrations ranging from 100% (undiluted whole blood) to 12.5% (diluted concentrations). An additional complementary contact angle measurement exhibits a monotonic decrease with a peak as a function of drying. This peak is related to a change in visco-elasticity that decreases with dilution, and disappears at the dilution concentration for the observed phase transition equivalent to 62% (v/v). This unique behavior is clearly commensurate with the optical image statistics and morphological analysis; and it is driven by the decrease in the interactions between various components within this bio-colloid. The implications of these phenomenal systems may address many open-ended questions of complex hierarchical structures.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Concentration-driven phase transition and self-assembly in drying droplets of diluting whole blood

Pal

Gope

Obayemi

et al. 2020

Sci Rep

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“…The higher intensity of C-D Raman band at the outer-ring indicate that deuteratedalbumin mainly locates at the outer-ring region of the dried droplet in agreement with the literature describing the distribution of protein at the periphery during the drying process [33,42]. It is possible that some fraction of non-deuterated albumin orients towards the inner region during the sample drying process.…”

Section: Distribution Of Abundant Blood Protein In the Dried Dropletsupporting

confidence: 89%

Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application

Huang

Ali

Quansah

et al. 2021

IJMS

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“…During the past decade, significant progress has been observed in the drying droplets of bio-colloids, such as [bovine serum albumin (BSA), and lysozyme] protein [3][4][5][6], plasma [7], DNA [8], serum [9], blood [10], etc., due to biomedical and forensic applications [9,[11][12][13][14][15]. The coupling between the droplet and the substrate, the surrounding environment, and the constituent particles show a wide variability range [16].…”

Section: Introductionmentioning

confidence: 99%

Temperature and Concentration Dependence of Human Whole Blood and Protein Drying Droplets

2021

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The drying of bio-colloidal droplets can be used in many medical and forensic applications. The whole human blood is the most complex bio-colloid system, whereas bovine serum albumin (BSA) is the simplest. This paper focuses on the drying characteristics and the final morphology of these two bio-colloids. The experiments were conducted by varying their initial concentrations, and the solutions were dried under various controlled substrate temperatures using optical and scanning electron microscopy. The droplet parameters (the contact angle, the fluid front, and the first-order image statistics) reveal the drying process’s unique features. Interestingly, both BSA and blood drying droplets’ contact angle measurements show evidence of a concentration-driven transition as the behavior changes from non-monotonic to monotonic decrease. This result indicates that this transition behavior is not limited to multi-component bio-colloid (blood) only, but may be a phenomenon of a bio-colloidal solution containing a large number of interacting components. The high dilution of blood behaves like the BSA solution. The ring-like deposition, the crack morphology, and the microstructures suggest that the components have enough time to segregate and deposit onto the substrate under ambient conditions. However, there is insufficient time for evaporative-driven segregation to occur at elevated temperatures, as expected.

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Understanding of the role of dilution on evaporative deposition patterns of blood droplets over hydrophilic and hydrophobic substrates

Cited by 34 publications

References 38 publications

Concentration-driven phase transition and self-assembly in drying droplets of diluting whole blood

Concentration-driven phase transition and self-assembly in drying droplets of diluting whole blood

Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application

Temperature and Concentration Dependence of Human Whole Blood and Protein Drying Droplets

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