Understanding opalescence measurements of biologics – A comparison study of methods, standards, and molecules

Kunz, Patrick; Stuckenberger, Eva; Hausmann, Kerstin; Gentiluomo, Lorenzo; Neustrup, Malene Aaby; Michalakis, Stylianos; Rieser, Ruth; Romeijn, Stefan; Wichmann, Christian; Windisch, Roland; Hawe, Andrea; Jiskoot, Wim; Menzen, Tim

doi:10.1016/j.ijpharm.2022.122321

Cited by 4 publications

(1 citation statement)

References 15 publications

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“…Several methods are available to determine the opalescence in a microplate, including a recently published method using a DLS/static light scattering (SLS) plate reader. 117 Therefore, opalescence measurements can be easily included in high-throughput screenings but cannot provide size or concentrations of protein particles.…”

Section: Orthogonal Approaches For the Selection Of Drug-like Antibodiesmentioning

confidence: 99%

Approaches to expand the conventional toolbox for discovery and selection of antibodies with drug-like physicochemical properties

Svilenov

Arosio

Menzen³

et al. 2023

mAbs

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Antibody drugs should exhibit not only high-binding affinity for their target antigens but also favorable physicochemical drug-like properties. Such drug-like biophysical properties are essential for the successful development of antibody drug products. The traditional approaches used in antibody drug development require significant experimentation to produce, optimize, and characterize many candidates. Therefore, it is attractive to integrate new methods that can optimize the process of selecting antibodies with both desired target-binding and drug-like biophysical properties. Here, we summarize a selection of techniques that can complement the conventional toolbox used to de-risk antibody drug development. These techniques can be integrated at different stages of the antibody development process to reduce the frequency of physicochemical liabilities in antibody libraries during initial discovery and to co-optimize multiple antibody features during early-stage antibody engineering and affinity maturation. Moreover, we highlight biophysical and computational approaches that can be used to predict physical degradation pathways relevant for long-term storage and in-use stability to reduce the need for extensive experimentation.

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Section: Orthogonal Approaches For the Selection Of Drug-like Antibodiesmentioning

confidence: 99%

Approaches to expand the conventional toolbox for discovery and selection of antibodies with drug-like physicochemical properties

Svilenov

Arosio

Menzen³

et al. 2023

mAbs

Self Cite

View full text Add to dashboard Cite

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A Versatile, Low-Cost Modular Microfluidic System to Prepare Poly(Lactic-co-Glycolic Acid) Nanoparticles With Encapsulated Protein

Neustrup,

Ottenhoff,

Jiskoot

et al. 2024

Pharm Res

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Combined Effect of Shaking Orbit and Vial Orientation on the Agitation-Induced Aggregation of Proteins

Dasnoy,

Illartin,

Queffelec

et al. 2024

Journal of Pharmaceutical Sciences

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Understanding opalescence measurements of biologics – A comparison study of methods, standards, and molecules

Cited by 4 publications

References 15 publications

Approaches to expand the conventional toolbox for discovery and selection of antibodies with drug-like physicochemical properties

Approaches to expand the conventional toolbox for discovery and selection of antibodies with drug-like physicochemical properties

A Versatile, Low-Cost Modular Microfluidic System to Prepare Poly(Lactic-co-Glycolic Acid) Nanoparticles With Encapsulated Protein

Combined Effect of Shaking Orbit and Vial Orientation on the Agitation-Induced Aggregation of Proteins

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