Understanding plant pathogen interactions using spatial and single-cell technologies

Zhu, Jie; Moreno-Pérez, Alba; Coaker, Gitta

doi:10.1038/s42003-023-05156-8

Cited by 18 publications

(1 citation statement)

References 101 publications

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“…This is because immune pathways are often self-amplified in transcriptional reprogramming (Li et al, 2016). Generally, pathogen distribution within host tissues is uneven, with multiple infection stages observed simultaneously, resulting in unequal symptom development (Zhu et al, 2023b). To address this, highthroughput single-cell RNA sequencing (scRNA-seq) was recently used to dissect plant responses to pathogens, revealing cellular heterogeneity in Arabidopsis-microbe interactions (Nobori et al, 2023;Zhu et al, 2023a;Delannoy et al, 2023;Tang et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Single-Cell Transcriptomic Analysis Highlights Specific Cell Types of Wheat Manipulated byFusarium graminearumLeading to Susceptibility

Wei,

Li,

Zhang

et al. 2024

Preprint

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Phytopathogenic fungi can be recognized by the plant immune system and trigger host defenses, but adapted pathogens cause susceptibility. How different cell types cooperate and orchestrate biological processes in response to heterogeneous colonization of organs by adapted and non-adapted pathogens remains largely unknown. Here we employed single-cell RNA sequencing to dissect the responses of wheat coleoptiles to infection by the adapted fungal pathogenFusarium graminearum(Fgr) and the non-adapted fungal pathogenFusarium oxysporumf. sp.cubense(Foc) at 1-, 2-, and 3-days post-inoculation. We profiled the transcriptomes of over 90,000 cells and identified eight major cell types in coleoptiles: stomata, epidermis, chlorenchyma, parenchyma, outer sheath, inner sheath, phloem, and procambium. Differential expression analyses showed that the capacity of different cell types to respond to fungal infection varied. The upregulation of immune pathways was compartmentalized in nonhost resistance toFoc, but widespread in susceptible interaction withFgr. Pseudotime analyses revealed continuous cell state transitions in the disease progression of infected cell types. Our work indicates that the phloem and outer sheath are specific cell types that collaborate for the rapid onset of nonhost resistance.Fgrinduces a state of low transcriptional activity in the chlorenchyma. Cell trajectory analysis suggests that the competition between immune and susceptible processes in parenchyma results in specific cell states that are favored by the adapted pathogenFgr. Overall, this work explains how cell types collaborate and are manipulated during fungal infections, providing insight into the intercellular mechanisms of plant immunity.

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