Understanding Protein–Nanoparticle Interaction: A New Gateway to Disease Therapeutics

Giri, Karuna; Shameer, Khader; Zimmermann, Michael T.; Saha, Sounik; Chakraborty, Prabir K.; Sharma, Anirudh; Arvizo, Rochelle R.; Madden, Benjamin J.; McCormick, Daniel J.; Kocher, Jean Pierre A.; Bhattacharya, Resham; Mukherjee, Priyabrata

doi:10.1021/bc500084f

Cited by 83 publications

(82 citation statements)

References 56 publications

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“…For example, the interfacial properties of inorganic nanoparticles is changed appreciably when they enter the GIT, which impacts cellular and tissue response to the nanoparticles. 117,118 Even after cellular uptake, inorganic nanoparticles may interact with cellular and/or blood proteins, which can further modify their biological fate. 76,[119][120][121] Enzyme activity: Gastrointestinal fluids contain digestive and metabolic enzymes that may change the properties of certain types of nanoparticles.…”

Section: Food Matrix and Git Effects On Nanoparticle Characteristics mentioning

confidence: 99%

Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles

2017

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Nanotechnology offers the food industry a number of new approaches for improving the quality, shelf life, safety, and healthiness of foods. Nevertheless, there is concern from consumers, regulatory agencies, and the food industry about potential adverse effects (toxicity) associated with the application of nanotechnology in foods. In particular, there is concern about the direct incorporation of engineered nanoparticles into foods, such as those used as delivery systems for colors, flavors, preservatives, nutrients, and nutraceuticals, or those used to modify the optical, rheological, or flow properties of foods or food packaging. This review article summarizes the application of both inorganic (silver, iron oxide, titanium dioxide, silicon dioxide, and zinc oxide) and organic (lipid, protein, and carbohydrate) nanoparticles in foods, highlights the most important nanoparticle characteristics that influence their behavior, discusses the importance of food matrix and gastrointestinal tract effects on nanoparticle properties, emphasizes potential toxicity mechanisms of different food-grade nanoparticles, and stresses important areas where research is still needed. The authors note that nanoparticles are already present in many natural and processed foods, and that new kinds of nanoparticles may be utilized as functional ingredients by the food industry in the future. Many of these nanoparticles are unlikely to have adverse affects on human health, but there is evidence that some of them could have harmful effects and that future studies are required.

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Section: Food Matrix and Git Effects On Nanoparticle Characteristics mentioning

confidence: 99%

Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles

2017

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“…The uptake of nanoparticles is determined by particle-protein and corona-membrane interactions, receptor-ligand binding interactions, membrane wrapping, biomolecule interaction and conformational change in the biomolecules. Recently Giri et al 29 have presented a detailed study of the composition of the protein coronas of gold nanoparticles at incubation times between 15 and 24 h. They showed that proteins adsorbed in the first 15 min have a significantly higher isoelectric point than the mean theoretical isoelectric point of all proteins present in the incubation media. 26 It was revealed that the presence of the corona ( protein layer) inhibits the formation of Aβ fibrils (which is the main cause of Alzheimer's diseases) 27 for all of the tested nanomaterials (e.g.…”

Section: Introductionmentioning

confidence: 99%

Significance of surface charge and shell material of superparamagnetic iron oxide nanoparticle (SPION) based core/shell nanoparticles on the composition of the protein corona

et al. 2015

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As nanoparticles (NPs) are increasingly used in many applications their safety and efficient applications in nanomedicine have become concerns. Protein coronas on nanomaterials' surfaces can influence how the cell "recognizes" nanoparticles, as well as the in vitro and in vivo NPs' behaviors. The SuperParamagnetic Iron Oxide Nanoparticle (SPION) is one of the most prominent agents because of its superparamagnetic properties, which is useful for separation applications. To mimic surface properties of different types of NPs, a core-shell SPION library was prepared by coating with different surfaces: polyvinyl alcohol polymer (PVA) ( positive, neutral and negative), SiO 2 ( positive and negative), titanium dioxide and metal gold. The SPIONs with different surfaces were incubated at a fixed serum : nanoparticle surface ratio, magnetically trapped and washed. The tightly bound proteins were quantified and identified. The surface charge has a great impact on protein adsorption, especially on PVA and silica where proteins preferred binding to the neutral and positively charged surfaces. The importance of surface material on protein adsorption was also revealed by preferential binding on TiO 2 and gold coated SPION, even negatively charged. There is no correlation between the protein net charge and the nanoparticle surface charge on protein binding, nor direct correlation between the serum proteins' concentration and the proteins detected in the coronas. † Electronic supplementary information (ESI) available. For ESI and crystallographic data in CIF or other electronic format see This journal is

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“…AuNP and protein interaction serve as starting point to study the biological effects of Au NPs. Usually, the high abundance proteins first arrive at and adsorb on the surface of NPs, but they are eventually replaced by high-affinity proteins to form NP-protein complexes [22]. AuNPs in biological fluids may form NP-protein complexes that can be [23].…”

Section: Resultsmentioning

confidence: 99%

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2018

RJLBPCS

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Nanomaterial stimulated alteration in the neuron is a great concern as the nanoparticles are widely used in the diagnosis of some neural disorders. Among the different nanoparticles, gold nanoparticles have a wide scope of applicability in the living system due to less toxicity. Gold nanoparticles can be used for cancer therapy especially for the killing of malignant cells in the brain. These particles can cross the bloodbrain barrier and can change the physiological functions of the brain. Gold nanoparticles may cause the change of different membrane proteins that might be responsible for defective transport of chemical through the membrane and finally leads to cellular loss. The experiment has been designed to study the changes of membrane polarity and intracellular calcium level in gold nanoparticles exposed cultured neuron. Citratestabilized gold nanoparticles are prepared from chloro-auric acid using sodium citrate as a stabilizing agent.Gold nanoparticles are characterized by UV Vis-spectrophotometer. Intracellular calcium level is monitored using fluorescence dye fluo 3. Membrane depolarization is studied using voltage-sensitive dye DiBAC(4)3. A higher level of intracellular calcium is observed in AuNP treated neurons. Elevation of intracellular calcium level might be due to the release of calcium from the intracellular storage or transport from extracellular media.Membrane depolarization and hyperpolarization study reveals that AuNP treated neurons membrane is present in depolarized state in comparison with the control neurons. A higher level of intracellular calcium and prolong depolarization in AuNP treated neuron may trigger some signaling mechanism leading to neuronal death

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Understanding Protein–Nanoparticle Interaction: A New Gateway to Disease Therapeutics

Cited by 83 publications

References 56 publications

Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles

Is nano safe in foods? Establishing the factors impacting the gastrointestinal fate and toxicity of organic and inorganic food-grade nanoparticles

Significance of surface charge and shell material of superparamagnetic iron oxide nanoparticle (SPION) based core/shell nanoparticles on the composition of the protein corona

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