Understanding RHE Creative Process

Kelley, Maurice J.

doi:10.1021/cen-v035n012.p016

Cited by 2 publications

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“…Consistency of the alignment process was confirmed on a daily basis in two ways: For light scatter, a panel of four polystyrene beads ranging in size from 0.48 to 1.43 m (Polysciences, Inc.) was analyzed, and peak channels were compared with previous results. For log FSC the largest bead appeared just above the 10 3 division on a four log scale (linear channels 1200 -1300) and the smallest beads at or below the 10 1 division (linear channels [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. The log SSC peaks ranged from the 10 2 (linear channels 70 -130) down to 10 1 (linear channels [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20].…”

Section: Preparation Of Fibroblast and Mouse Tissue Extracts Subcellmentioning

confidence: 99%

The Beige/Chediak-Higashi Syndrome Gene Encodes a Widely Expressed Cytosolic Protein

Perou

Leslie

Green

et al. 1997

Journal of Biological Chemistry

128

102

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The human autosomal recessive disorder ChediakHigashi syndrome and its murine homologue beige are associated with the formation of giant lysosomes that cluster near the perinuclear region of cells. We prepared a polyclonal antiserum against a glutathione S-transferase-Beige fusion protein and demonstrated by Western analysis that the beige gene encodes a protein of 400 kDa that is expressed in cultured murine fibroblasts as well as most mouse tissues. The protein was not detected in either cultured fibroblasts or mouse tissues from two different beige mutants. Cultured fibroblasts transformed with multiple copies of yeast artificial chromosomes that contain the full-length beige gene showed much higher levels of Beige protein than either wild type fibroblasts or mouse tissues. Subcellular fractionation experiments demonstrated that the Beige protein was cytosolic and, under the conditions of isolation, had no measurable membrane association. Cultured mouse fibroblasts in which the Beige protein was overexpressed had smaller than normal lysosomes that were more peripherally distributed than in control cells. These findings, coupled with earlier published results, suggest that the Beige protein regulates lysosomal fission.

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Section: Preparation Of Fibroblast and Mouse Tissue Extracts Subcellmentioning

confidence: 99%

The Beige/Chediak-Higashi Syndrome Gene Encodes a Widely Expressed Cytosolic Protein

Perou

Leslie

Green

et al. 1997

Journal of Biological Chemistry

128

102

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“…The most investigated animal models are murine equivalents of CHS. A radiation-induced mutation resulted in the first described Lyst -mutant mouse, deemed the beige (bg or Lyst bg ) mouse for its silvery-grey coat coloration compared to C57BL/6J wild-type (WT) mice ( 47 , 48 ). The spontaneous mutation of beige allele is caused by either i.…”

Section: Animal Models Of Lyst Mutationsmentioning

confidence: 99%

The lysosomal trafficking regulator “LYST”: an 80-year traffic jam

Turner,

Che,

Mirhaidari

et al. 2024

Front. Immunol.

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Lysosomes and lysosome related organelles (LROs) are dynamic organelles at the intersection of various pathways involved in maintaining cellular hemostasis and regulating cellular functions. Vesicle trafficking of lysosomes and LROs are critical to maintain their functions. The lysosomal trafficking regulator (LYST) is an elusive protein important for the regulation of membrane dynamics and intracellular trafficking of lysosomes and LROs. Mutations to the LYST gene result in Chédiak-Higashi syndrome, an autosomal recessive immunodeficiency characterized by defective granule exocytosis, cytotoxicity, etc. Despite eight decades passing since its initial discovery, a comprehensive understanding of LYST’s function in cellular biology remains unresolved. Accumulating evidence suggests that dysregulation of LYST function also manifests in other disease states. Here, we review the available literature to consolidate available scientific endeavors in relation to LYST and discuss its relevance for immunomodulatory therapies, regenerative medicine and cancer applications.

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Understanding RHE Creative Process

Cited by 2 publications

References 0 publications

The Beige/Chediak-Higashi Syndrome Gene Encodes a Widely Expressed Cytosolic Protein

The Beige/Chediak-Higashi Syndrome Gene Encodes a Widely Expressed Cytosolic Protein

The lysosomal trafficking regulator “LYST”: an 80-year traffic jam

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