Understanding the distribution of antibiotic resistance genes in an urban community using wastewater-based epidemiological approach

Javvadi, Yamini; Mohan, S. Venkata

doi:10.1016/j.scitotenv.2023.161419

Cited by 12 publications

(6 citation statements)

References 72 publications

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“…4 . At a phylum level, communities were dominated by Pseudomonadota followed by Bacteriodota , which is consistent with previous 16S rRNA gene amplicon-based studies on plastic associated biofilms and wastewater [ 17 , 59 – 62 ]. Like in those studies, statistical tests offered a clear clustering at early timepoints which become dispersed at later timepoints as mature biofilms formed [ 18 ].…”

Section: Resultssupporting

confidence: 90%

“…AMR gene incidence: effects of abiotic factors and and sampling location Our findings of AMR classes abundances were consistent with those reported by Javvadi and Mohan [ 62 ], with large proportions accounted for loci for resistance to by tetracyclines, β-lactams and MLSBs (macrolides). Additionally, “Universal ARG”, mphE , msrE , tetA , tetC , tetW , sul1 and sul2 were detected in wastewater samples, consistently with Munk et al [ 27 ], although not all of them persisted on plastic surfaces, or even after river- and seawater incubations.…”

Section: Resultssupporting

confidence: 90%

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Microbial communities colonising plastics during transition from the wastewater treatment plant to marine waters

Tulloch,

Bargiela,

Williams

et al. 2024

Environmental Microbiome

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Background Plastics pollution and antimicrobial resistance (AMR) are two major environmental threats, but potential connections between plastic associated biofilms, the ‘plastisphere’, and dissemination of AMR genes are not well explored. Results We conducted mesocosm experiments tracking microbial community changes on plastic surfaces transitioning from wastewater effluent to marine environments over 16 weeks. Commonly used plastics, polypropylene (PP), high density polyethylene (HDPE), low density polyethylene (LDPE) and polyethylene terephthalate (PET) incubated in wastewater effluent, river water, estuarine water, and in the seawater for 16 weeks, were analysed via 16S rRNA gene amplicon and shotgun metagenome sequencing. Within one week, plastic-colonizing communities shifted from wastewater effluent-associated microorganisms to marine taxa, some members of which (e.g. Oleibacter-Thalassolituus and Sphingomonas spp., on PET, Alcanivoracaceae on PET and PP, or Oleiphilaceae, on all polymers), were selectively enriched from levels undetectable in the starting communities. Remarkably, microbial biofilms were also susceptible to parasitism, with Saprospiraceae feeding on biofilms at late colonisation stages (from week 6 onwards), while Bdellovibrionaceae were prominently present on HDPE from week 2 and LDPE from day 1. Relative AMR gene abundance declined over time, and plastics did not become enriched for key AMR genes after wastewater exposure. Conclusion Although some resistance genes occurred during the mesocosm transition on plastic substrata, those originated from the seawater organisms. Overall, plastic surfaces incubated in wastewater did not act as hotspots for AMR proliferation in simulated marine environments.

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Section: Resultssupporting

confidence: 90%

Section: Resultssupporting

confidence: 90%

Microbial communities colonising plastics during transition from the wastewater treatment plant to marine waters

Tulloch,

Bargiela,

Williams

et al. 2024

Environmental Microbiome

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“…(2) Amplicon-directed surveys, which, unlike plating, capture the diversity of cultivable and uncultivable bacteria and can assay for the presence of any ARG whose sequence is known. However, this method does not divulge which bacterial strains or species carry resistance markers, and any conclusions about the association between bacteria and ARGS are, at best, correlative (Danko et al 2021 , Javvadi and Mohan 2023 ). (3) Metagenomic surveys, which can reveal the complement of genes and strains in a community (Forslund et al 2014 , Crofts et al 2017 ), but entail the assembly of large contigs to associate an ARG with a specific genome and are not suitable for genes residing on plasmids, which disassociate from host genomes during metagenomic library preparation.…”

Section: Discussionmentioning

confidence: 99%

Examining the taxonomic distribution of tetracycline resistance in a wastewater plant

Ochman,

Quandt,

Gottell

et al. 2024

Sustainable Microbiology

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Microbial communities serve as reservoirs of antibiotic resistance genes (ARGs) and facilitate the dissemination of these genes to bacteria that infect humans. Relatively little is known about the taxonomic distribution of bacteria harboring ARGs in these reservoirs and the avenues of transmission due to the technical hurdles associated with characterizing the contents of complex microbial populations and the assignment of genes to particular genomes. Focusing on the array of tetracycline resistance (Tcr) genes in the primary and secondary phases of wastewater treatment, 17 of the 22 assayed Tcr genes were detected in at least one sample. We then applied epicPCR to link tetracycline resistance genes to specific bacterial hosts. Whereas Tcr genes tend to vary in their distributions among bacterial taxa according to their modes of action, there were numerous instances in which a particular Tcr gene was associated with a host that was distantly related to all other bacteria bearing the same gene, including several hosts not previously identified. Tcr genes are far less host-restricted than previously assumed, indicating that complex microbial communities serve as settings where ARGs are spread among divergent bacterial phyla.

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“…Establishing a system for conducting nationwide monitoring surveys will provide useful information for clarifying the impact of AMR on the environments [21][22][23]. However, surveillance methods for AMR and antimicrobials in the environment are still in the early stages of global development, and many unknown factors remain in Japan [24][25][26][27]. Therefore, an urgent need prevails to establish a survey method to quantify and estimate the environmental risk to humans and animals and elucidate AMR mechanisms and transmission pathways [28,29].…”

Section: Introductionmentioning

confidence: 99%

Development of a High-Throughput Analytical Method for Antimicrobials in Wastewater Using an Automated Pipetting and Solid-Phase Extraction System

Azuma,

Matsunaga,

Ohmagari

et al. 2024

Antibiotics

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Antimicrobial resistance (AMR) has emerged and spread globally. Recent studies have also reported the presence of antimicrobials in a wide variety of aquatic environments. Conducting a nationwide monitoring survey of AMR in the environment to elucidate its status and to assess its impact on ecosystems and human health is of social importance. In this study, we developed a novel high-throughput analysis (HTA) system based on a 96-well plate solid-phase extraction (SPE), using automated pipetting and an SPE pre-treatment system. The effectiveness of the system as an HTA for antimicrobials in environmental water was verified by comparing it with a conventional manual analytical system in a domestic hospital over a period of two years and four months. The results of the manual analysis and HTA using a combination of automated pipetting and SPE systems were generally consistent, and no statistically significant difference was observed (p > 0.05) between the two systems. The agreement ratios between the measured concentrations based on the conventional and HTA methods were positively correlated with a correlation coefficient of r = 0.99. These results indicate that HTA, which combines automated pipetting and an SPE pre-treatment system for rapid, high-volume analysis, can be used as an effective approach for understanding the environmental contamination of antimicrobials at multiple sites. To the best of our knowledge, this is the first report to present the accuracy and agreement between concentrations based on a manual analysis and those measured using HTA in hospital wastewater. These findings contribute to a comprehensive understanding of antimicrobials in aquatic environments and assess the ecological and human health risks associated with antimicrobials and antimicrobial-resistant bacteria to maintain the safety of aquatic environments.

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Understanding the distribution of antibiotic resistance genes in an urban community using wastewater-based epidemiological approach

Cited by 12 publications

References 72 publications

Microbial communities colonising plastics during transition from the wastewater treatment plant to marine waters

Microbial communities colonising plastics during transition from the wastewater treatment plant to marine waters

Examining the taxonomic distribution of tetracycline resistance in a wastewater plant

Development of a High-Throughput Analytical Method for Antimicrobials in Wastewater Using an Automated Pipetting and Solid-Phase Extraction System

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