Understanding the Dynamics of the Saccharomyces cerevisiae and Scheffersomyces stipitis Abundance in Co-culturing Process for Bioethanol Production from Corn Stover

Wu, Yilu; Wen, Jieyi; Wang, Kang; Su, Changsheng; Chen, Changjing; Cui, Ziheng; Cai, Di; Cheng, Shikun; Cao, Huibo; Qin, Peiyong

doi:10.1007/s12649-022-01861-3

Cited by 7 publications

(13 citation statements)

References 51 publications

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“…anomalus HCJ2F-19. In agreement with our findings, Wu et al (2023) found the highest substrate conversion rate to ethanol with an inoculum size of 10% and inoculum ratio of 60:40 of S.…”

Section: Optimization Of Fermentation Conditionssupporting

confidence: 93%

“…4a and b), whereas the mono-culture of S. cerevisiae TA2 and W. anomalus HCJ2F-19 required 48 h to produce a bioethanol concentration of 17.2 g L -1 and 14.5 g.L -1 , and ethanol yield 0.33 g.g -1 and 0.28 g.g -1 , respectively. According to Wu et al (2023) an ethanol concentration of 15.4 g.L -1 was obtained at the end of the co-culturing process of Scheffersomyces stipites and S. cerevisiae, which is lower than the current finding. During co-fermentation, in about 12 h an increase of 29% and 53% for the bioethanol yield was obtained when compared to the monocultures of S. cerevisiae TA2 and W. anomalus HCJ2F-19, respectively.…”

Section: Co-fermentation Of S Cerevisiae Ta2 and W Anomalus Hcj2f-19contrasting

confidence: 81%

“…The co-fermentation of S. cerevisiae TA2 and W. anomalus HCJ2F-19 was carried out at pH 4.6, 30 o C, 30 o brix, 2 g L -1 DAP supplement, mixing rate 150 rpm, incubation period 72 h (Hawaz et al, 2022), inoculum ratio of (20/80, in v/v) and an overall inoculum size of 10% (v/v) (Wu et al, 2023). The yeast S. cerevisiae TA2 (highly ethanol tolerant) was first inoculated with a volume of 200 mL (2.9 x 10 8 cells/mL) (Fig.…”

Section: Co-culture Fermentationmentioning

confidence: 99%

“…Firstly, the effect of the inoculum ratio and overall inoculum size on alcohol production was examined by varying the volume of the inoculum (0/100, 5/95, 20/80, 40/60, 60/40, 80/20, 95/5, and 100/0, in v/v), and 10-35 (% (v/v)), respectively (Wu et al, 2023), while keeping all other variables constant. The pH of the fermentation medium was optimized by varying the pH value from 3.5 to 6.5 while the optimum value of the inoculum size and inoculum ratio was maintained.…”

Section: One Variable At a Time Optimizationmentioning

confidence: 99%

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Bioethanol production from sugarcane molasses by co-fermentation of Saccharomyces cerevisiae TA2 and Wickerhamomyces anomalus HCJ2F-19

Hawaz

Tafesse

Tesfaye

et al. 2023

Preprint

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Purpose Co-culturing of stress-tolerant fermenting yeasts is a widely used method to improve bioethanol production from biomass enriched in fermentable sugars. This study aims to produce bioethanol from sugarcane molasses by simultaneous co-fermentation of S. cerevisiae isolate TA2 and W. anomalus isolate HCJ2F-19. Method Response surface methodology (RSM) based on the central composite design (CCD) was employed to optimize fermentation conditions, including mixing rate (110–150 rpm), temperature (25–35 oC), molasses concentration (25–35 obrix), and incubation time (36–72 h). The ethanol concentration was analyzed using HPLC equipped with a UV detector. Results The mono-culture, S. cerevisiae TA2 produces 17.2 g.L− 1 of ethanol, 0.33 g.g− 1 of ethanol yield, and 0.36 g.L− 1.h− 1 of productivity compared to W. anomalus HCJ2F which produces 14.5 g.L− 1, 0.30 g.g− 1 and 0.28 g.L− 1.h− 1 ethanol, ethanol yield, and productivity under laboratory conditions, respectively. In comparison to single cultures of S. cerevisiae TA2, and W. anomalus HCJ2F, the co-fermentation showed an increased ethanol yield of 29% and 53% compared to the single species fermentations, respectively. The results showed that the growth of W. anomalus HCJ2F-19 and S. cerevisiae TA2 was not influenced by each other during the co-fermentation process. The one variable at a time optimization (OVAT) demonstrated an ethanol concentration of 26.5 g.L− 1 with a specific yield and productivity of 0.46 g.g− 1, 0.55 g.L− 1.h− 1, respectively, at pH 5.5, 25 obrix, 48 h, 150 rpm, 30oC, 60:40 inoculum ratio, and 10% overall inoculum size. The maximum ethanol concentration of 35.5 g.L− 1 was obtained by co-fermentation using the RSM-CCD tool at 30 obrix, 30oC, 54 h, and 130 rpm. Conclusion The results suggested that the co-fermentation of S. cerevisiae TA2 and W. anomalus HCJ2F improves bioethanol production under optimum fermentation conditions.

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“…anomalus HCJ2F-19. In agreement with our findings, Wu et al (2023) found the highest substrate conversion rate to ethanol with an inoculum size of 10% and inoculum ratio of 60:40 of S.…”

Section: Optimization Of Fermentation Conditionssupporting

confidence: 93%

Section: Co-fermentation Of S Cerevisiae Ta2 and W Anomalus Hcj2f-19contrasting

confidence: 81%

Section: Co-culture Fermentationmentioning

confidence: 99%

Section: One Variable At a Time Optimizationmentioning

confidence: 99%

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Bioethanol production from sugarcane molasses by co-fermentation of Saccharomyces cerevisiae TA2 and Wickerhamomyces anomalus HCJ2F-19

Hawaz

Tafesse

Tesfaye

et al. 2023

Preprint

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“…The engineered xylose-assimilating strain (name YL23) after ALE that with high xylose metabolism efficiency was screened and adopted for bioethanol fermentation using the RCF pulps’ hydrolysate. The fermentations were carried out without any other nutrients’ supplementation under oxygen limitation conditions in 250 mL shake flasks containing 50 mL substrate [ 64 ]. The inoculation rate of the YL23 seed was 10% (v/v, cultured in YPD medium, the OD 600 was at ~ 1).…”

Section: Methodsmentioning

confidence: 99%

Sequential catalytic lignin valorization and bioethanol production: an integrated biorefinery strategy

Wu,

Su,

Liao

et al. 2024

Biotechnol Biofuels

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Background The effective valorization of lignin and carbohydrates in lignocellulose matrix under the concept of biorefinery is a primary strategy to produce sustainable chemicals and fuels. Based on the reductive catalytic fractionation (RCF), lignin in lignocelluloses can be depolymerized into viscous oils, while the highly delignified pulps with high polysaccharides retention can be transformed into various chemicals. Results A biorefinery paradigm for sequentially valorization of the main components in poplar sawdust was constructed. In this process, the well-defined low-molecular-weight phenols and bioethanol were co-generated by tandem chemo-catalysis in the RCF stage and bio-catalysis in fermentation stage. In the RCF stage, hydrogen transfer reactions were conducted in one-pot process using Raney Ni as catalyst, while the isopropanol (2-PrOH) in the initial liquor was served as a hydrogen donor and the solvent for lignin dissolution. Results indicated the proportion of the 2-PrOH in the initial liquor of RCF influenced the chemical constitution and yield of the lignin oil, which also affected the characteristics of the pulps and the following bioethanol production. A 67.48 ± 0.44% delignification with 20.65 ± 0.31% of monolignols yield were realized when the 2-PrOH:H2O ratio in initial liquor was 7:3 (6.67 wt% of the catalyst loading, 200 °C for 3 h). The RCF pulp had higher carbohydrates retention (57.96 ± 2.78 wt%), which was converted to 21.61 ± 0.62 g/L of bioethanol with a yield of 0.429 ± 0.010 g/g in fermentation using an engineered S. cerevisiae strain. Based on the mass balance analysis, 104.4 g of ethanol and 206.5 g of lignin oil can be produced from 1000 g of the raw poplar sawdust. Conclusions The main chemical components in poplar sawdust can be effectively transformed into lignin oil and bioethanol. The attractive results from the biorefinery process exhibit great promise for the production of valuable biofuels and chemicals from abundant lignocellulosic materials.

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Current advances of the valorization technologies for heavy metal containing hyperaccumulators

Wen,

Zhou,

Zhang

et al. 2024

Industrial Crops and Products

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Understanding the Dynamics of the Saccharomyces cerevisiae and Scheffersomyces stipitis Abundance in Co-culturing Process for Bioethanol Production from Corn Stover

Cited by 7 publications

References 51 publications

Bioethanol production from sugarcane molasses by co-fermentation of Saccharomyces cerevisiae TA2 and Wickerhamomyces anomalus HCJ2F-19

Bioethanol production from sugarcane molasses by co-fermentation of Saccharomyces cerevisiae TA2 and Wickerhamomyces anomalus HCJ2F-19

Sequential catalytic lignin valorization and bioethanol production: an integrated biorefinery strategy

Current advances of the valorization technologies for heavy metal containing hyperaccumulators

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