Understanding the Effects of Trenbolone Acetate, Polyamine Precursors, and Polyamines on Proliferation, Protein Synthesis Rates, and the Abundance of Genes Involved in Myoblast Growth, Polyamine Biosynthesis, and Protein Synthesis in Murine Myoblasts

Motsinger, Laura A; Okamoto, Lillian L; Ineck, Nikole E; Udy, Brynne A; Erickson, Christopher L.; Harraq, Youssef; Reichhardt, Caleb C; Murdoch, Gordon K.; Thornton, Kara J

doi:10.3390/biology12030446

Cited by 2 publications

(2 citation statements)

References 71 publications

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“…RNA isolation from the cells was carried out using the Absolutely RNA Micro prep Kit (Agilent Technologies, Cedar Creek, TX, USA) as previously described [ 37 , 39 ]. Following the removal of media for protein analysis, the cells were washed twice with 1 mL of cold (4°C) PBS containing 1× ABAM.…”

Section: Methodsmentioning

confidence: 99%

Expression and secretion of SPARC, FGF-21 and DCN in bovine muscle cells: Effects of age and differentiation

Shira,

Thornton,

Murdoch

et al. 2024

PLoS ONE

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Skeletal muscle growth is an economically important trait in the cattle industry. Secreted muscle-derived proteins, referred to as myokines, have important roles in regulating the growth, metabolism, and health of skeletal muscle in human and biomedical research models. Accumulating evidence supports the importance of myokines in skeletal muscle and whole-body health, though little is known about the potential presence and functional significance of these proteins in cattle. This study evaluates and confirms that secreted proteins acidic and rich in cysteine (SPARC), fibroblast growth factor 21 (FGF-21), myostatin (MSTN), and decorin (DCN) are expressed and SPARC, FGF-21, and DCN are secreted by primary bovine satellite cells from 3- (BSC3; n = 3) and 11- (BSC11; n = 3) month -old commercial angus steers. Cells were cultured and collected at zero, 12, 24, and 48 hours to characterize temporal expression and secretion from undifferentiated and differentiated cells. The expression of SPARC was higher in the undifferentiated (p = 0.04) and differentiated (p = 0.07) BSC11 than BSC3. The same was observed with protein secretion from undifferentiated (p <0.0001) BSC11 compared to BSC3. Protein secretion of FGF-21 was higher in undifferentiated BSC11 (p < 0.0001) vs. BSC3. DCN expression was higher in differentiated BSC11 (p = 0.006) vs. BSC3. Comparing undifferentiated vs. differentiated BSC, MSTN expression was higher in differentiated BSC3 (p ≤ 0.001) for 0, 12, and 24 hours and in BSC11 (p ≤ 0.03) for 0, 12, 24, and 48 hours. There is also a change over time for SPARC expression (p ≤ 0.03) in undifferentiated and differentiated BSC and protein secretion (p < 0.0001) in undifferentiated BSC, as well as FGF-21 expression (p = 0.007) in differentiated BSC. This study confirms SPARC, FGF-21, and DCN are secreted, and SPARC, FGF-21, MSTN, and DCN are expressed in primary bovine muscle cells with age and temporal differences.

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Section: Methodsmentioning

confidence: 99%

Expression and secretion of SPARC, FGF-21 and DCN in bovine muscle cells: Effects of age and differentiation

Shira,

Thornton,

Murdoch

et al. 2024

PLoS ONE

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show abstract

“…The data were reported as protein quantified per volume of media. Absolutely RNA Miniprep Kits (Agilent Technologies, Cedar Creek, TX, USA) were used for RNA isolation from cells as previously described [35,37]. After RNA isolation, quantification took place using a NanoDrop™ One instrument (ThermoFisher Scientific Inc., Waltham, MA, USA).…”

Section: Protein Analysismentioning

confidence: 99%

Myokines Produced by Cultured Bovine Satellite Cells Harvested from 3- and 11-Month-Old Angus Steers

Shira,

Murdoch,

Thornton

et al. 2024

Animals

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The myokines interleukin 6 (IL-6), interleukin 15 (IL-15), myonectin (CTRP15), fibronectin type III domain containing protein 5/irisin (FNDC5), and brain-derived neurotrophic factor (BDNF) are associated with skeletal muscle cell proliferation, differentiation, and muscle hypertrophy in biomedical model species. This study evaluated whether these myokines are produced by cultured bovine satellite cells (BSCs) harvested from 3- and 11-month-old commercial black Angus steers and if the expression and secretion of these targets change across 0, 12, 24, and 48 h in vitro. IL-6, IL-15, FNDC5, and BDNF expression were greater (p ≤ 0.05) in the differentiated vs. undifferentiated BSCs at 0, 12, 24, and 48 h. CTRP15 expression was greater (p ≤ 0.03) in the undifferentiated vs. differentiated BSCs at 24 and 48 h. IL-6 and CTRP15 protein from culture media were greater (p ≤ 0.04) in undifferentiated vs. differentiated BSCs at 0, 12, 24, and 48 h. BDNF protein was greater in the media of differentiated vs. undifferentiated BSCs at 0, 12, 24, and 48 h. IL-6, 1L-15, FNDC5, and BDNF are expressed in association with BSC differentiation, and CTRP15 appears to be expressed in association with BSC proliferation. This study also confirms IL-6, IL-15, CTRP15, and BDNF proteins present in media collected from primary cultures of BSCs.

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Understanding the Effects of Trenbolone Acetate, Polyamine Precursors, and Polyamines on Proliferation, Protein Synthesis Rates, and the Abundance of Genes Involved in Myoblast Growth, Polyamine Biosynthesis, and Protein Synthesis in Murine Myoblasts

Cited by 2 publications

References 71 publications

Expression and secretion of SPARC, FGF-21 and DCN in bovine muscle cells: Effects of age and differentiation

Expression and secretion of SPARC, FGF-21 and DCN in bovine muscle cells: Effects of age and differentiation

Myokines Produced by Cultured Bovine Satellite Cells Harvested from 3- and 11-Month-Old Angus Steers

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