Understanding the Mechanisms Involved in the Regulation of Cytochrome P450 Gene Expression in Drosophila melanogaster (Diptera: Drosophilidae)

Br, Mohammed; Simon, Marissa; Mn, Opara; Oc, Jegede; Ris, Agbede; Finn, Robert D.

doi:10.4172/2161-0983.1000189

Entomol Ornithol Herpetol

2016

DOI: 10.4172/2161-0983.1000189

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Understanding the Mechanisms Involved in the Regulation of Cytochrome P450 Gene Expression in Drosophila melanogaster (Diptera: Drosophilidae)

Mohammed Br¹,

Marissa Simon²,

Opara Mn³

et al.

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Cited by 2 publications

References 63 publications

(86 reference statements)

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Unveiling the Role of Two Rhodopsin-like GPCR Genes in Insecticide-Resistant House Flies, Musca domestica

Xin,

Brown,

Wang

et al. 2024

IJMS

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Insecticide resistance in insects, driven by the overexpression of P450 enzymes, presents a significant challenge due to the enhanced metabolic detoxification of insecticides. Although the transcriptional regulation of P450 genes is not yet fully understood, G-protein-coupled receptor (GPCR) genes have emerged as key regulators in this process. This study is the first to associate GPCR genes with insecticide resistance in Musca domestica. We identified two key rhodopsin-like GPCR genes, ALHF_02706.g1581 and ALHF_04422.g2918, which were significantly overexpressed in the resistant ALHF strain compared to sensitive strains. Notably, both ALHF_02706.g1581 and ALHF_04422.g2918 were mapped to autosome 2, where critical but unidentified regulatory factors controlling resistance and P450 gene regulation are located. This supports our hypothesis that GPCRs function as trans-regulatory factors for P450-mediated resistance. Functional analysis using transgenic Drosophila demonstrated that overexpression of these rhodopsin-like GPCR genes increased permethrin resistance by approximately two-fold. Specifically, ALHF_02706.g1581 overexpression significantly upregulated the Drosophila resistance-related P450 genes CYP12D1, CYP6A2, and CYP6A8, while ALHF_04422.g2918 increased CYP6G1 and CYP6A2 expression, thereby enhancing insecticide detoxification in rhodopsin-like GPCR transgenic Drosophila lines. These findings suggest that these rhodopsin-like GPCR genes on autosome 2 may act as trans-regulatory factors for P450-mediated resistance, underscoring their critical role in insecticide detoxification and resistance development in M. domestica.

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Unveiling the Role of Two Rhodopsin-like GPCR Genes in Insecticide-Resistant House Flies, Musca domestica

Xin,

Brown,

Wang

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

Cytochrome P450s in Anopheles gambiae (Diptera: Culicidae) and Insecticide Resistance in Africa: A Mini Review

Mohammed¹,

Mailafia²,

Malang³

et al. 2017

Entomol Ornithol Herpetol

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Cytochrome P450s are known to be critical for the detoxification and/or activation of xenobiotics such as insecticides in all living organisms including Anopheles gambiae. Many studies have demonstrated the role of P450s in insecticide resistance in A. gambiae. However, little is known about the impact of distribution in the African subcontinent. In this paper therefore, we analyse the P450 clans, the CYP6 family, localisation and function of A. gambiae CYPs, their insecticide substrates, regional distribution in the African continent and their role in insecticide resistance. This investigation from published data revealed that in the Central region; CYP6Z3, CYP6Z1, CYP12F2, CYP6P4, CYP6GA1, CYP6Z3 (Yaoundé, Cameroun) have bendiocarb, DDT and pyrethroids as substrates; in the Eastern region: CYP314A1 and CYP12F1 (Tanzania and Zanzibar) have DDT as a substrate, CYP32A3, CYP6Z1 and CYP6Z2 (Western Kenya) have DDT, carbaryl and permethrin; whilst in the Western region: CYP6AG1, CYP6Z2, CYP6Z3, CY6P3, CYP6P4, CYP6M2 (Ghana), CYP6M2, CYP6P3 (Benin), CYP325A3, CYP6P3 and CYP6M2 (Nigeria) all have DDT, carbaryl, permethrin, trans-and cis-permethrin, deltamethrin, bendiocarb as substrates. Additionally, CYP6M2, CYP6P3, CYP6Z3 (Côte d'Ivoire), CYP6P3, CYP6Z2 and CYP9J5 (Burkina Faso) have bendiocarb, DDT plus pyrethroids and only pyrethroids as substrates respectively. Interestingly, CYP6P3 is observed to metabolize all the available insecticides (DDT, pyrethroid, trans-and cis-permethrin, deltamethrin and bendiocarb), indicating possible insecticide cross resistance across all the three regions of Africa. A more detailed understanding of the substrate specificities of various P450s and the geographical distribution of insecticide resistance in Africa is quintessential for an effective resistance management.

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Understanding the Mechanisms Involved in the Regulation of Cytochrome P450 Gene Expression in Drosophila melanogaster (Diptera: Drosophilidae)

Cited by 2 publications

References 63 publications

Unveiling the Role of Two Rhodopsin-like GPCR Genes in Insecticide-Resistant House Flies, Musca domestica

Unveiling the Role of Two Rhodopsin-like GPCR Genes in Insecticide-Resistant House Flies, Musca domestica

Cytochrome P450s in Anopheles gambiae (Diptera: Culicidae) and Insecticide Resistance in Africa: A Mini Review

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