Understanding the molecular basis of adaptation to freshwater environments by prawns in the genus Macrobrachium

Lifat, Rahi

doi:10.5204/thesis.eprints.118051

Cited by 9 publications

(9 citation statements)

References 223 publications

(689 reference statements)

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“…The stable pattern of gene expression is maintained in subsequent generations in a heritable fashion (Lee at al., 2011; Towle, Henry, & Erwilliger, 2011; Velotta et al., 2015). For the increased expression of candidate genes, crustaceans require more energy; individuals usually consume more food and O 2 (to enhance metabolic activity) to meet the growing demand of energy (Aziz et al., 2018; Rahi, 2017; Rosas et al., 2001; Shock et al., 2009; Wang et al., 2019). A major change can occur in crustacean food habit due to large fluctuation in environmental salinity levels (Aziz et al., 2017; Long et al., 2017; Moshtaghi et al., 2016; Rahi et al., 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The principal mechanism for ionic balance (or regulation) in crustaceans is osmoregulation which involves: efficient ion transport (for active ion uptake or secretion depending on the surrounding aquatic medium), body fluid (haemolymph) regulation, osmotic stress response and changes in metabolic performance through increased O 2 consumption (Faleiros, Goldman, Furriel, & McNamara, 2010; McNamara, Freire, Torres, & Faria, 2015; Moshtaghi, Rahi, Nguyen, Mather, & Hurwood, 2016; Rahi, 2017; Rahi, Moshtaghi, Mather, & Hurwood, 2018; Rivera‐Ingraham & Lignot, 2017). These physiological activities are accomplished efficiently by changing the expression pattern of underlying candidate genes (Havird, Santos, & Henry, 2014; Lv et al., 2013; Moshtaghi et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

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Impact of salinity changes on growth, oxygen consumption and expression pattern of selected candidate genes in the orange mud crab ( Scylla olivacea )

et al. 2020

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Change in environmental salinity level is a major limiting factor for the aquaculture productivity because it imposes severe stress on organisms that in turn retards growth. The orange mud crab (Scylla olivacea) is an important coastal aquaculture species (farming is practised in 10‰–20‰ salinity levels) in Bangladesh. The present study was conducted to investigate the changes in growth, O2 consumption and mRNA expression levels of five selected genes in the orange mud crab (S. olivacea) exposed to three different experimental salinity levels (0‰, 10‰ and 20‰) for three months. Crabs reared at 10‰ and 20‰, showed significantly higher (p < .05) growth performance and expression of growth regulatory genes (Actin and α‐amylase). The highest levels (p < .05) of O2 consumption and expression of ion regulatory genes (Na+‐K+‐ATPase, V‐type H+‐ATPase and Diuretic Hormone) were obtained at 0‰. Moderate levels of growth and expression of selected candidate genes were observed at 10‰ treatment while the highest levels of growth and gene expression were obtained at 20‰ (control salinity). Strong interactions were observed between growth performance and expression of growth genes (R2 = 0.81–0.91), and rate of O2 consumption and expression of ion regulatory genes (R2 = 0.83–0.93), implying that the selected genes are important candidates for growth and ionic balance in S. olivacea. Growth performance was found to be very low at 0‰ initially, after 30 days crabs showed better growth performance at this salinity level. It is thus inferred that orange mud crab individuals might require 3–5 days for acclimation to salinity stress but it can take at least 30 days for acclimation to regular growth. Results indicate that with proper acclimation, the orange mud crab (Scylla olivacea) can be farmed at low salinity conditions and possibly in freshwater condition.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Impact of salinity changes on growth, oxygen consumption and expression pattern of selected candidate genes in the orange mud crab ( Scylla olivacea )

et al. 2020

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“…Triploids usually contain 33% additional genetic material in each nucleus (Rahi & Shah, 2012a;Tave, 1992;Vargas et al, 2015). This could potentially increase their heterozygosity (Altimiras et al, 2002;Pujolar et al, 2006;Rahi, 2017) and favour the superior growth performance of triploid individuals (Borell et al, 2004;Garner et al, 2008;Islam et al, 2014;Leary et al, 1985;Malison et al, 1993;Rahi et al, 2019). Sterility and the presence of extra genetic material are reported to be the main reasons for the better growth performance of triploids over their diploid counterparts (Felip et al, 2001;Johnson et al, 2007;Leary et al, 1985;Moshtaghi et al, 2017;Shah et al, 1999;Tave, 1992;Utter et al, 1983).…”

Section: Growth Performance In Culture Pondsmentioning

confidence: 99%

Growth and survival of diploid and triploid bata, Labeo bata (Hamilton, 1822)

Afroz

Shah

Salin³

et al. 2021

Aquaculture Fish & Fisheries

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Bata (Labeo bata), an indigenous, freshwater minor carp, is a great delicacy in Bangladesh. In its natural fishery, bata contributes significantly to the health and nutritional security of a large section of the fisher communities in Bangladesh. However, it is not a popular farmed fish because of its small size and slow growth rate. The induction of triploidy, aimed at providing faster growth and larger body size, could be appropriate for L. bata to enhance its aquaculture potential. In the present study, we induced triploidy in L. bata by applying heat shock on fertilised eggs (that were maintained at the ambient temperature of 26-28 • C), 3 to 5 min post fertilisation and for durations ranging from 1 to 2 min. Four different temperatures, namely, 38, 39, 40 and 41 • C, were tested as heat shock treatments. The optimum treatment combination for triploidy induction in L. bata was found to be 39 • C for the 1 min duration, 3 min after fertilisation, yielding 100% triploidy. Karyotyping revealed 72 chromosomes in the triploid fish (3n), whereas the diploid control (2n) had 48 chromosomes. The growth performance of the triploid hatchlings was compared against the diploid control in experimental ponds.After 12 weeks of rearing, the triploid fish were significantly heavier (p < 0.05) than their diploid counterparts. The survival rate of the triploids was also better (69%) than that of diploids (56%). Overall, results revealed better performance (growth and survival) of triploid bata over diploids, indicating that the farming of triploid bata can be a viable practice in Bangladesh.

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“…2005; Freire et al. 2008; Rahi 2017). Crustacean taxa exhibit ample habitat diversity and representatives are found in a diverse array of aquatic habitats from fresh to marine waters (Vogt 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Depending on life history traits, Macrobrachium species are categorized into two groups: abbreviated larval development (ALD) type and extended larval development (ELD) type (Short 2004). ALD species can complete entire life cycle in freshwater, are characterized by having only a few (20–250) large eggs, and have only 1–3 larval developmental stages (Rahi 2017). ELD species in contrast require brackish or sea water to complete larval development, have numerous smaller eggs (500–10,000), and many (8–15) larval developmental stages (Murphy and Austin 2005; Wowor et al.…”

Section: Introductionmentioning

confidence: 99%

The Molecular Basis of Freshwater Adaptation in Prawns: Insights from Comparative Transcriptomics of Three Macrobrachium Species

Lifat

Mather

Ezaz

et al. 2019

Genome Biology and Evolution

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Elucidating the molecular basis of adaptation to different environmental conditions is important because adaptive ability of a species can shape its distribution, influence speciation, and also drive a variety of evolutionary processes. For crustaceans, colonization of freshwater habitats has significantly impacted diversity, but the molecular basis of this process is poorly understood. In the current study, we examined three prawn species from the genus Macrobrachium ( M. australiense , M . tolmerum , and M . novaehollandiae ) to better understand the molecular basis of freshwater adaptation using a comparative transcriptomics approach. Each of these species naturally inhabit environments with different salinity levels; here, we exposed them to the same experimental salinity conditions (0‰ and 15‰), to compare expression patterns of candidate genes that previously have been shown to influence phenotypic traits associated with freshwater adaptation (e.g., genes associated with osmoregulation). Differential gene expression analysis revealed 876, 861, and 925 differentially expressed transcripts under the two salinities for M . australiense , M . tolmerum , and M . novaehollandiae , respectively. Of these, 16 were found to be unannotated novel transcripts and may be taxonomically restricted or orphan genes. Functional enrichment and molecular pathway mapping revealed 13 functionally enriched categories and 11 enriched molecular pathways that were common to the three Macrobrachium species. Pattern of selection analysis revealed 26 genes with signatures of positive selection among pairwise species comparisons. Overall, our results indicate that the same key genes and similar molecular pathways are likely to be involved with freshwater adaptation widely across this decapod group; with nonoverlapping sets of genes showing differential expression (mainly osmoregulatory genes) and signatures of positive selection (genes involved with different life history traits).

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Understanding the molecular basis of adaptation to freshwater environments by prawns in the genus Macrobrachium

Cited by 9 publications

References 223 publications

Impact of salinity changes on growth, oxygen consumption and expression pattern of selected candidate genes in the orange mud crab ( Scylla olivacea )

Impact of salinity changes on growth, oxygen consumption and expression pattern of selected candidate genes in the orange mud crab ( Scylla olivacea )

Growth and survival of diploid and triploid bata, Labeo bata (Hamilton, 1822)

The Molecular Basis of Freshwater Adaptation in Prawns: Insights from Comparative Transcriptomics of Three Macrobrachium Species

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