Understanding the regulation of extracellular protease gene expression in fungi: a key step towards their biotechnological applications

Snyman, Carla; Theron, Louwrens Wiid; Divol, Benoit

doi:10.1007/s00253-019-09902-z

Cited by 24 publications

(17 citation statements)

References 114 publications

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“…Several GO terms related to proteolysis, possibly related to the low nitrogen levels (Snyman et al, 2019), used to avoid repression of ligninolytic activities, were enriched in transcripts that were higher on the S-related aromatics (Table S2). From the transcripts higher on the G-related aromatics, GO terms related to polysaccharide degradation were enriched although most of these polysaccharide degrading CAZymes were not highly expressed (Table S1E).…”

Section: Differentially Expressed Transcripts On G-compared To S-relamentioning

confidence: 99%

Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens

Daly

Peng

López

et al. 2020

Journal of Biotechnology

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Heterologous production of fungal ligninolytic cocktails is challenging due to the low yields of catalytically active lignin modifying peroxidases. Production using a natural system, such as a wood-rotting fungus, is a promising alternative if specific or preferential induction of the ligninolytic activities could be achieved. Using transcriptomics, gene expression of the white-rot Dichomitus squalens during growth on mixtures of aromatic compounds, with ring structures representing the two major lignin sub-units, was compared to a wood substrate. Most of the genes encoding lignin modifying enzymes (laccases and peroxidases) categorised as highly or moderately expressed on wood were expressed similarly on aromatic compounds. Higher expression levels of a subset of manganese and versatile peroxidases was observed on di-compared to mono-methoxylated aromatics. The expression of polysaccharide degrading enzymes was lower on aromatic compounds compared to wood, demonstrating that the induction of lignin modifying enzymes became more specific. This study suggests potential for aromatic waste streams, e.g. from lignocellulose pretreatment, to produce a lignin-specific enzyme cocktail from D. squalens or other white-rot fungi.

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Section: Differentially Expressed Transcripts On G-compared To S-relamentioning

confidence: 99%

Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens

Daly

Peng

López

et al. 2020

Journal of Biotechnology

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“…Filamentous fungi are renowned as a potential producer of proteases, for they can secrete proteases in low‐cost fermentation media and be genetically engineered easily [2]. Thus, the regulation of extracellular protease production by filamentous fungi has gained increasing attention in recent years [3].…”

Section: Introductionmentioning

confidence: 99%

“…regulation of extracellular protease production by filamentous fungi has gained increasing attention in recent years [3].…”

mentioning

confidence: 99%

“…Protease production is highly susceptible to diverse environmental stimuli, especially nutrient limitations including that of nitrogen and carbon sources [3][4][5]. It has been reported that the expression of protease genes is prevented in the presence of preferred nitrogen sources, which is controlled by nitrogen catabolite repression (NCR) pathway [6].…”

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confidence: 99%

“…Moreover, the regulation of protease production by nitrogen sources relies on GATA-type zinc finger transcription factors, such as areA in Aspergillus nidulans, are1 in Trichoderma reesei, and nit-2 in Neurospora crassa [7][8][9]. In addition, that carbon starvation leads to the increase of protease gene expression has been demonstrated in specific filamentous fungi, in which transcriptional factors creA and xprG are involved [3,10,11]. However, knowledge concerning the complicated mechanisms of regulation of protease production is limited and previous studies have focused on only certain species of filamentous fungi, mostly pathogenic organisms [3].…”

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confidence: 99%

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Extracellular protease production regulated by nitrogen and carbon sources in Trichoderma reesei

et al. 2021

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The filamentous fungus Trichoderma reesei is an important producer of industrial enzymes, and possesses abundant extracellular protease genes based on the genome sequence data. However, the production of extracellular proteases remains poorly understood. Here, protease production was extensively investigated on different carbon (glucose and lactose) and nitrogen sources ((NH 4) 2 SO 4 , NaNO 3 , peptone, and corn steep liquor). It was found that protease production was dominantly regulated by nitrogen sources. Organic nitrogen sources were beneficial for protease production, while the preferred nitrogen source (NH 4) 2 SO 4 inhibited the expression of proteases. As for carbon sources, lactose was a more effective inducer than glucose for protease production. The protease activity was further examined by protease inhibitors, which suggested that protease activity was predominantly inhibited by phenylmethanesulfonyl fluoride (PMSF) and slightly suppressed by ethylenediaminetetraacetic acid (EDTA). Moreover, proteomic analysis revealed a total of 29 extracellular proteases, including 13 serine proteases, 6 aspartic proteases, and 10 metalloproteases. In addition, seven proteases were found to be present among all conditions. These results showed the regulatory profile of extracellular protease production in Trichoderma reesei grown on various carbon and nitrogen sources, which will facilitate the development of T. reesei to be an effective workhorse for enzyme or high-value protein production in industry.

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Identification of mycoparasitism-related genes in Trichoderma harzianum T4 that are active against Colletotrichum musae

Wang,

Wang

2023

Arch Microbiol

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Understanding the regulation of extracellular protease gene expression in fungi: a key step towards their biotechnological applications

Cited by 24 publications

References 114 publications

Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens

Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens

Extracellular protease production regulated by nitrogen and carbon sources in Trichoderma reesei

Identification of mycoparasitism-related genes in Trichoderma harzianum T4 that are active against Colletotrichum musae

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