Understanding the strategic landscape surrounding the implementation of mass spectrometry in the clinical laboratory: A SWOT analysis

Vogeser, Michael; Zhang, Y. Victoria

doi:10.1016/j.clinms.2018.06.001

Cited by 17 publications

(4 citation statements)

References 19 publications

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“…Particularly, a great interest has been directed to a saccharolipid containing carotenoid, known as Staphyloxanthin (STX) (Braungardt and Singh, 2019;Perez-Lopez et al, 2019;Tiwari et al, 2018;Xue et al, 2019), a natural pigment with wellknown antioxidant properties (Mishra et al, 2011;Tiwari et al, 2018;Zhang et al, 2018), responsible for the characteristic color of S. aureus (Kim and Lee, 2012;Marshall and Wilmoth, 1981a), and associated with tolerance to oxidative stress (Clauditz et al, 2006;Liu et al, 2005;Olivier et al, 2009). However, STX also plays an essential role on the regulation of membrane mechanical properties, and has been shown to hinder the permeability of the membrane to cationic antimicrobial peptides, increasing the virulence and bacterial fitness of S. aureus (Crass et al, 2019;Mishra et al, 2011;Vogeser and Zhang, 2018). In the first in S. aureus have been developed by fluorescence spectroscopy using the probe 1,6-diphenyl-1,3,5-hexatriene (DPH) (Mishra et al, 2011;Perez-Lopez et al, 2019;Sen et al, 2016;Tiwari et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Carotenogenesis of Staphylococcus aureus: New insights and impact on membrane biophysical properties

López

Suesca

Álvarez‐Rivera

et al. 2021

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Staphyloxanthin (STX) is a saccharolipid derived from a carotenoid in Staphylococcus aureus involved in oxidative-stress tolerance and antimicrobial peptide resistance. In this work, a targeted metabolomics and biophysical study was carried out on native and knock-out S. aureus strains to investigate the biosynthetic pathways of STX and related carotenoids. Identification of 34 metabolites at different growth phases (8, 24 and 48h), reveal shifts of carotenoid populations during progression towards stationary phase.Six of the carotenoids in the STX biosynthetic pathway and three menaquinones (Vitamin K2) were identified in the same chromatogram. Furthermore, other STX homologues with varying acyl chain structures reported herein for the first time, which reveal the extensive enzymatic activity of CrtO/CrtN. Fourier Transform infrared spectroscopy show that STX increases acyl chain order and shifts the cooperative melting of the membrane indicating a more rigid lipid bilayer. This study shows the diversity of carotenoids in S. aureus, and their influence on membrane biophysical properties.

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Section: Introductionmentioning

confidence: 99%

Carotenogenesis of Staphylococcus aureus: New insights and impact on membrane biophysical properties

López

Suesca

Álvarez‐Rivera

et al. 2021

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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“…La cromatografía líquida acoplada a la espectrometría de masas ha ganado en popularidad en los laboratorios clínicos en los últimos 10–15 años [ 1 , 2 ]. El aumento de su utilidad probablemente se deba a su mayor especificidad como técnica analítica, la posibilidad de determinar múltiples analitos en una sola inyección de la muestra, la medición de analitos en una variedad de matrices diferentes y a una drástica reducción de las interferencias analíticas en comparación con el inmunoensayo [ 3 ]. Sin embargo, el inmunoensayo todavía se utiliza habitualmente en muchos laboratorios como prueba de cribado de primera línea, si bien cualquier resultado positivo debe ser confirmado por un segundo método basado en la cromatografía acoplada a la espectrometría de masas [ 4 ].…”

Section: Introductionunclassified

Optimización de un método de cribado rápido de fármacos en sangre mediante la técnica de cromatografía de líquidos acoplada a espectrometría de masas

Rodrigo Valero,

Quintela Jorge,

Bravo Serrano

et al. 2023

Advances in Laboratory Medicine / Avances en Medicina De Laboratorio

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Resumen Objetivos La cromatografía líquida acoplada a la espectrometría de masas ha ganado en popularidad en los laboratorios en los últimos años debido a una mayor especificidad de la técnica, la posibilidad de determinar múltiples analitos en una sola inyección de la muestra, la medición de analitos en una variedad de matrices diferentes y a una drástica reducción de las interferencias analíticas en comparación con el inmunoensayo. El tratamiento y preparación de las muestras biológicas es un proceso esencial cuando éstas han de ser analizadas mediante sistemas cromatográficos. Los principales interferentes en el análisis de las muestras de sangre son los fosfolípidos y las proteínas. El objetivo principal de este estudio es mejorar la sistemática analítica toxicológica en el cribado general de fármacos mediante la técnica LC-MS/MS a través de un nuevo método de preparación de muestras en sangre basado en la precipitación de proteínas y eliminación de fosfolípidos. Métodos Se ha evaluado el nuevo método de preparación de muestras en sangre basado en la precipitación de proteínas y eliminación de fosfolípidos mediante la tecnología LC-Q-q-LIT. Resultados Se ha determinado el límite de detección, el límite de cuantificación y rango de medición para las 56 moléculas seleccionadas y se han comparado los resultados de once casos con las extracciones e instrumentación tradicionales. Conclusiones La metodología propuesta de preparación de muestras en sangre y análisis mediante técnicas más sensibles como LC-Q-q-LIT ha resultado comparable a la metodología tradicional en cuanto a resultados y, ofreciendo, además, una reducción de tiempo y coste.

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“…In the last 10–15 years, liquid chromatography-tandem mass spectrometry has gained popularity in clinical laboratories [ 1 , 2 ]. Benefits of this analytical technique include a higher specificity, the possibility of measuring different analytes in a single sample injection, measuring analytes in different matrices, and a dramatic reduction of analytical interferences, as compared to immunoassay [ 3 ]. Notably, immunoassay is still used in many laboratories as the gold-standard method for screening drugs in blood.…”

Section: Introductionmentioning

confidence: 99%

Optimization of a rapid method for screening drugs in blood by liquid chromatography tandem mass spectrometry

Rodrigo Valero,

Jorge,

Serrano

et al. 2023

Advances in Laboratory Medicine / Avances en Medicina De Laboratorio

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Objectives In the recent years, liquid chromatography with tandem mass spectrometry has gained popularity in laboratories. This technique has a higher specificity, detects different analytes from a single specimen, measures analytes in distinct matrices, and substantially reduce analytical interference, with respect to immunoassay. The processing and preparation of biological samples are crucial in chromatography. Interferences in blood testing are usually caused by the presence of phospholipids and proteins. The main objective of this study was to improve analytical processes for drug screening by LC-MS/MS using a novel blood sample preparation method based on protein precipitation and removal of phospholipids. Methods An evaluation was performed of a new method for the preparation of blood samples based on protein precipitation and removal of phospholipids by LC-Q-q-LIT. Results Limit of detection, limit of quantification and measurement range were determined for 56 molecules. The results of 11 cases were compared with those obtained using standard blood collection methods and instruments. Conclusions The novel blood preparation and testing method based on LC-Q-q-LIT, a more sensitive technique, has demonstrated to yield comparable results to traditional methods. In addition, this new technique reduces turnaround time and costs.

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Understanding the strategic landscape surrounding the implementation of mass spectrometry in the clinical laboratory: A SWOT analysis

Cited by 17 publications

References 19 publications

Carotenogenesis of Staphylococcus aureus: New insights and impact on membrane biophysical properties

Carotenogenesis of Staphylococcus aureus: New insights and impact on membrane biophysical properties

Optimización de un método de cribado rápido de fármacos en sangre mediante la técnica de cromatografía de líquidos acoplada a espectrometría de masas

Optimization of a rapid method for screening drugs in blood by liquid chromatography tandem mass spectrometry

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