Undibacterium crateris sp. nov., isolated from water of crater lake

Abstract

A Gram-stain-negative, aerobic, flagellated, non-spore-forming, rod-shaped bacterium, named B2R-29T, was isolated from water collected from a crater lake on Da Hinggan mountain, PR China. Strain B2R-29T was oxidase- and catalase-positive. On the basis of the results of 16S rRNA gene sequence analyses, strain B2R-29T clearly belonged to the family Oxalobacteraceae , class Betaproteobacteria and showed the highest similarity to Undibacterium oligocarboniphilum EM1T (97.4 %) and to the other species of Undibacterium (less than 96.8 %). In the phylogenetic tree, strain B2R-29T formed a clade with U. oligocarboniphilum EM1T and Undibacterium squillarum CMJ-9T, indicating that is a member of the genus Undibacterium . Digital DNA–DNA hybridization and average nucleotide identity analyses were performed and the values between strain B2R-29T and its closely related Undibacterium species were less than 75.1 % and 16.9 %, respectively. The chemotaxonomic data of B2R-29T were as follows: major uniquinone, Q-8; predominant polar lipids, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol; major fatty acids, C16 : 0 and summed feature 3 (C16 : 1 ω7c / C16 : 1 ω6c); predominant polyamines, putrescine, 2-hydroxyputrescine and spermidine. The DNA G+C content was 51.7 mol% from the genomic sequencing data. In accordance with the phenotypic, physiological and chemotaxonomic properties mentioned above, strain B2R-29T represents a novel species of the genus Undibacterium for which the name Undibacterium crateris sp. nov. is proposed. The type strain is B2R-29T (=CGMCC 1.13792T=KCTC 72018T).

Undibacterium baiyunense sp. nov., Undibacterium curvum sp. nov., Undibacterium fentianense sp. nov., Undibacterium flavidum sp. nov., Undibacterium griseum sp. nov., Undibacterium hunanense sp. nov., Undibacterium luofuense sp. nov., Undibacterium nitidum sp. nov., Undibacterium rivi sp. nov., Undibacterium rugosum sp. nov. and Undibacterium umbellatum sp. nov., isolated from streams in China

“…All the polar lipids profiles of twelve strains consisted of phosphatidylethanolamine, phosphatidylglycerol, one unidentified phospholipid and two kinds of unidentified aminolipids, that were similar with profiles of other members within genus Undibacterium (Fig. S7) [2–10].…”

“…Strains CY7W T , CY18W T , CY22W T , FT31W T , FT137W T , FT147W T , BYS50W, BYS107W T , LFS511W T , LX15W T , LX22W T and NL8W T contained Q-8 as the major respiratory quinone, similar to the respiratory quinone profiles of other members within genus Undibacterium [1–10]. The cellular fatty acid profiles were homogeneous among twelve strains and eight reference strains.…”

“…in 2011 [2]. At the time of writing, 14 novel species within this genus were reported and these species were mainly isolated from water environments (drinking water, purified water, waterfall, lake, stream and shrimp culture pond) [1–8]. Some species were also found in soil and gut samples [9, 10].…”