Undifferentiated Cells of Tessaria absinthioides with High Nutritional Value and Health-Promoting Phytochemicals. An Approach Based on Plant Cellular Agriculture

Martinez, Antares A.; Panuska, Camila; Kurina-Sanz, Marcela; Rinaldoni, Ana N.; Orden, Alejandro A.

doi:10.1007/s11130-023-01105-2

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…In the last few years, large scale cultivation of plant in vitro systems has been considered as a prospective branch of cellular agriculture, a technology for sustainable manufacture of agricultural goods by using single cells without involving plants or animals [38][39][40][41][42][43][44]. Recently, plant cells were used for production of cosmetic ingredients, plant-based proteins and even for food products as chocolate and coffee [39][40][41][42][45][46][47]. However, the low productivity and the high cost of produced biomass are among the major limiting factors of this technology.…”

Section: Introductionmentioning

confidence: 99%

Light Can Induce Accumulation of Nutritional Antioxidants in Black Chokeberry Cell Suspension Culture

Krasteva,

Teneva-Angelova,

Badjakov

et al. 2023

Applied Sciences

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Cultivation of plant cells under controlled conditions is a highly effective and fast developing technology that recently was considered as a branch of cellular agriculture. Cellular agriculture is a multidisciplinary approach for sustainable and renewable production of agricultural goods and raw materials at cellular, rather than organismal, level. However, in contrast to the rapid advance in development of cultured meat and precision fermentation, the production of nutritional supplements from plant cells is still in its infancy. One of the limiting factors, striating commercialization of plant cells for food production, is the low yields of target bioactive metabolites. In this work, the changes in phenolics, anthocyanins and exopolysaccharides accumulation during cultivation of Aronia melanocarpa (Michx.) Elliott cell suspension cultures in darknessor under illumination (16 light and 8 dark) were investigated. The data showed that the highest contents of total phenolics (8.17 ± 0.39 mg GAE/g DW), total anthocyanins (0.011 ± 0.001 mg cyanidin-3-glucoside equivalents/g DW) and antioxidant activities (DPPH—21.36 ± 0.29 µM TE/g DW; TEAC—10.08 ± 0.07 µM TE/g DW; FRAP—34.85 ± 1.47 µM TE/g DW; and CUPRAC—126.74 ± 9.15 µM TE/g DW) were achieved when the cells were grown under illumination (16 light and 8 dark). In contrast, when the culture was grown indarkness, the highest amounts of accumulated dry biomass (8.68 ± 0.35 g/L) and exopolysaccharides production (2.10 ± 0.07 g/L) were reached. The results demonstrated that light can be used as an affordable and highly effective factor to control the production of valuable antioxidants by black chokeberry cell suspension culture.

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Section: Introductionmentioning

confidence: 99%

Light Can Induce Accumulation of Nutritional Antioxidants in Black Chokeberry Cell Suspension Culture

Krasteva,

Teneva-Angelova,

Badjakov

et al. 2023

Applied Sciences

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Light-mediated elicitation of Tessaria absinthioides calli for the production of anthocyanins: correlation with protein expression

Martinez,

Bonilla,

Aguilera

et al. 2024

Plant Cell Tiss Organ Cult

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Light-Mediated Elicitation of Tessaria absinthioides Calli for the Production of Anthocyanins: Correlation with Protein Expression

Martinez,

Bonilla,

Aguilera

et al. 2024

Preprint

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This study explores the metabolic responses of undifferentiated cells of Tessaria absinthioides to light elicitation, with a focus on the production and accumulation of anthocyanins. We observed that anthocyanin concentrations in cell biomass were notably higher compared to those in wild flowers, albeit with slight compositional differences, according to the HPLC-DAD analysis. In a proteomic analysis of T. absinthioidescalli, light exposure induced significant changes in protein expression, with 578 proteins overexpressed and 486 uniquely identified under this condition. These proteins were predominantly clustered in key functional categories like protein processing, cellular transport, and DNA regulation, along with those involved in sesquiterpene synthesis and developmental regulation. Furthermore, proteins crucial for the phenylpropanoid pathway and the anthocyanin biosynthesis were identified in light-exposed calli, with many significantly overexpressed compared to dark-grown cultures. A similar trend was observed in enzymes associated with the de novo synthesis of arginine, a precursor to stress-responsive metabolites like polyamines and nitric oxide. These findings help elucidate the metabolic pathways underlying anthocyanin production in T. absinthioides. They also extend to the development of natural dyes, highlighting the significance of cellular culture in sustainable bioproduction.

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Undifferentiated Cells of Tessaria absinthioides with High Nutritional Value and Health-Promoting Phytochemicals. An Approach Based on Plant Cellular Agriculture

Cited by 3 publications

References 25 publications

Light Can Induce Accumulation of Nutritional Antioxidants in Black Chokeberry Cell Suspension Culture

Light Can Induce Accumulation of Nutritional Antioxidants in Black Chokeberry Cell Suspension Culture

Light-mediated elicitation of Tessaria absinthioides calli for the production of anthocyanins: correlation with protein expression

Light-Mediated Elicitation of Tessaria absinthioides Calli for the Production of Anthocyanins: Correlation with Protein Expression

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