Uneven HAK/KUP/KT Protein Diversity Among Angiosperms: Species Distribution and Perspectives

Nieves‐Cordones, Manuel; Ródenas, Reyes; Chavanieu, Alain; Rivero, Rosa M.; Martı́nez, Vicente; Gaillard, Isabelle; Rubio, Francisco

doi:10.3389/fpls.2016.00127

Cited by 70 publications

(82 citation statements)

References 49 publications

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“…These findings suggest that ZmHAK5 transcription may be regulated by a different mechanism compared with homologs in other plant species, or perhaps different maize inbred lines utilize distinct mechanisms for ZmHAK5 regulation. The genetic diversity and complexity of maize inbred lines might result in differences such as these (Nieves‐Cordones et al ). Exploring the natural variations in the promoter region of ZmHAK5 may provide additional information about its transcriptional regulation.…”

Section: Discussionmentioning

confidence: 99%

ZmHAK5 and ZmHAK1 function in K⁺ uptake and distribution in maize under low K⁺ conditions

Qin

Wang

2019

JIPB

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Potassium (K+) is an essential macronutrient for plant growth and development. Transporters from the KT/HAK/KUP family play crucial roles in K+ homeostasis and cell growth in various plant species. However, their physiological roles in maize are still unknown. In this study, we cloned ZmHAK5 and ZmHAK1 and investigated their functions in maize (Zea mays L.). In situ hybridization showed that ZmHAK5 was mainly expressed in roots, especially in the epidermis, cortex, and vascular bundle. ZmHAK5 was characterized as a high‐affinity K+ transporter. Loss of function of ZmHAK5 led to defective K+ uptake in maize, under low K+ conditions, whereas ZmHAK5‐overexpressing plants showed increased K+ uptake activity and improved growth. ZmHAK1 was upregulated under low K+ stress, as revealed by RT‐qPCR. ZmHAK1 mediated K+ uptake when heterologously expressed in yeast, but its transport activity was weaker than that of ZmHAK5. Overexpression of ZmHAK1 in maize significantly affected K+ distribution in shoots, leading to chlorosis in older leaves. These findings indicate that ZmHAK5 and ZmHAK1 play distinct roles in K+ homeostasis in maize, functioning in K+ uptake and K+ distribution, respectively. Genetic manipulation of ZmHAK5 may represent a feasible way to improve K+ utilization efficiency in maize.

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Section: Discussionmentioning

confidence: 99%

ZmHAK5 and ZmHAK1 function in K⁺ uptake and distribution in maize under low K⁺ conditions

Qin

Wang

2019

JIPB

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“…In clade IIc, AtKUP2/6/8 negatively regulates plant growth and cell size via mediating K + efflux rather than influx (Osakabe et al, 2013; Nieves-Cordones et al, 2016). Another clade IIb gene is AtKUP1/KT1, the first cloned HAK/KT/KUP transporter from Arabidopsis ; however, its physiological role has not yet been determined (Kim et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

In-Depth Genomic and Transcriptomic Analysis of Five K+ Transporter Gene Families in Soybean Confirm Their Differential Expression for Nodulation

et al. 2017

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Plants have evolved a sophisticated network of K+ transport systems to regulate growth and development. Limited K+ resources are now forcing us to investigate how plant demand can be satisfied. To answer this complex question, we must understand the genomic and transcriptomic portfolio of K+ transporters in plants. Here, we have identified 70 putative K+ transporter genes from soybean, including 29 HAK/KT/KUP genes, 16 genes encoding voltage-gated K+ channels, 9 TPK/KCO genes, 4 HKT genes, and 12 KEA genes. To clarify the molecular evolution of each family in soybean, we analyzed their phylogeny, mode of duplication, exon structures and splice sites, and paralogs. Additionally, ortholog clustering and syntenic analysis across five other dicots further explored the evolution of these gene families and indicated that the soybean data is suitable as a model for all other legumes. Available microarray data sets from Genevestigator about nodulation was evaluated and further confirmed with the RNA sequencing data available by a web server. For each family, expression models were designed based on Transcripts Per Kilobase Million (TPM) values; the outcomes indicated differential expression linked to nodulation and confirmed the genes' putative roles. In-depth studies such as ours provides the basis for understanding K+ inventories in all other plants.

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“…OsHAK1 belongs to cluster Ia of HAK/KUP/KT K + transporters (Nieves‐Cordones et al ., ), whose members are widely associated with root high‐affinity K + uptake in several plant species beside rice, for example, barley, Arabidopsis, tomato, pepper and Eutrema salsugineum (Santa‐María et al ., ; Rubio et al ., ; Bañuelos et al ., ; Martínez‐Cordero et al ., ; Nieves‐Cordones et al ., ; Alemán et al ., ). OsHAK1 localizes to the plasma membrane and is upregulated in roots under low K + conditions (Bañuelos et al ., ; Chen et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Production of low‐Cs⁺ rice plants by inactivation of the K⁺ transporter OsHAK1 with the CRISPR‐Cas system

et al. 2017

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The occurrence of radiocesium in food has raised sharp health concerns after nuclear accidents. Despite being present at low concentrations in contaminated soils (below μm), cesium (Cs ) can be taken up by crops and transported to their edible parts. This plant capacity to take up Cs from low concentrations has notably affected the production of rice (Oryza sativa L.) in Japan after the nuclear accident at Fukushima in 2011. Several strategies have been put into practice to reduce Cs content in this crop species such as contaminated soil removal or adaptation of agricultural practices, including dedicated fertilizer management, with limited impact or pernicious side-effects. Conversely, the development of biotechnological approaches aimed at reducing Cs accumulation in rice remain challenging. Here, we show that inactivation of the Cs -permeable K transporter OsHAK1 with the CRISPR-Cas system dramatically reduced Cs uptake by rice plants. Cs uptake in rice roots and in transformed yeast cells that expressed OsHAK1 displayed very similar kinetics parameters. In rice, Cs uptake is dependent on two functional properties of OsHAK1: (i) a poor capacity of this system to discriminate between Cs and K ; and (ii) a high capacity to transport Cs from very low external concentrations that is likely to involve an active transport mechanism. In an experiment with a Fukushima soil highly contaminated with Cs , plants lacking OsHAK1 function displayed strikingly reduced levels of Cs in roots and shoots. These results open stimulating perspectives to smartly produce safe food in regions contaminated by nuclear accidents.

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Uneven HAK/KUP/KT Protein Diversity Among Angiosperms: Species Distribution and Perspectives

Cited by 70 publications

References 49 publications

ZmHAK5 and ZmHAK1 function in K⁺ uptake and distribution in maize under low K⁺ conditions

ZmHAK5 and ZmHAK1 function in K⁺ uptake and distribution in maize under low K⁺ conditions

In-Depth Genomic and Transcriptomic Analysis of Five K+ Transporter Gene Families in Soybean Confirm Their Differential Expression for Nodulation

Production of low‐Cs⁺ rice plants by inactivation of the K⁺ transporter OsHAK1 with the CRISPR‐Cas system

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Uneven HAK/KUP/KT Protein Diversity Among Angiosperms: Species Distribution and Perspectives

Cited by 70 publications

References 49 publications

ZmHAK5 and ZmHAK1 function in K+ uptake and distribution in maize under low K+ conditions

ZmHAK5 and ZmHAK1 function in K+ uptake and distribution in maize under low K+ conditions

In-Depth Genomic and Transcriptomic Analysis of Five K+ Transporter Gene Families in Soybean Confirm Their Differential Expression for Nodulation

Production of low‐Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR‐Cas system

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ZmHAK5 and ZmHAK1 function in K⁺ uptake and distribution in maize under low K⁺ conditions

ZmHAK5 and ZmHAK1 function in K⁺ uptake and distribution in maize under low K⁺ conditions

Production of low‐Cs⁺ rice plants by inactivation of the K⁺ transporter OsHAK1 with the CRISPR‐Cas system