Unexpected competitiveness of Methanosaeta populations at elevated acetate concentrations in methanogenic treatment of animal wastewater

Chen, Si; Cheng, Huicai; Liu, Jiang; Hazen, Terry C.; Huang, Vicki; He, Qiang

doi:10.1007/s00253-016-7967-9

Cited by 39 publications

(15 citation statements)

References 37 publications

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“…The limited archaeal diversity may have been due to the lack of niches for microorganisms in coal to inhabit. Other studies have demonstrated the difficulties of amplifying archaeal 16S rRNA gene sequences from coal samples 31 ; however the primer set 515F/806R is known to cover methanogens in wastewater treatment 32 and diverse archaeal communities in coal seams in China 33 . The low archaeal sequence reads may indicate low concentrations of methanogens from the samples, which may result in the observed long lag phase of gas production (Fig.…”

Section: Resultsmentioning

confidence: 99%

Low carbon renewable natural gas production from coalbeds and implications for carbon capture and storage

et al. 2017

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Isotopic studies have shown that many of the world’s coalbed natural gas plays are secondary biogenic in origin, suggesting a potential for gas regeneration through enhanced microbial activities. The generation of biogas through biostimulation and bioaugmentation is limited to the bioavailability of coal-derived compounds and is considered carbon positive. Here we show that plant-derived carbohydrates can be used as alternative substrates for gas generation by the indigenous coal seam microorganisms. The results suggest that coalbeds can act as natural geobioreactors to produce low carbon renewable natural gas, which can be considered carbon neutral, or perhaps even carbon negative depending on the amount of carbon sequestered within the coal. In addition, coal bioavailability is no longer a limiting factor. This approach has the potential of bridging the gap between fossil fuels and renewable energy by utilizing existing coalbed natural gas infrastructure to produce low carbon renewable natural gas and reducing global warming.

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Section: Resultsmentioning

confidence: 99%

Low carbon renewable natural gas production from coalbeds and implications for carbon capture and storage

et al. 2017

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“…Members of these orders were also found in the community in the pilot-scale reactor, but in low relative abundances. Methanothrix species are described to play an important role in the formation and maintenance of a strong granular sludge (MacLeod et al, 1990;McHugh et al, 2005) and it is believed to be dominant at low acetate concentrations (De Vrieze et al, 2012), but it was also recently reported to be able to become dominant at high acetate concentrations (Chen and He, 2015;Chen et al, 2017). Methanobacteriaceae species members could be found in total community, but their presence in the activity community was very low (∼1%).…”

Section: Discussionmentioning

confidence: 99%

Microbial Community Redundancy and Resilience Underpins High-Rate Anaerobic Treatment of Dairy-Processing Wastewater at Ambient Temperatures

Paulo

Castilla-Archilla

Ramiro-García

et al. 2020

Front. Bioeng. Biotechnol.

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High-rate anaerobic digestion (AD) is a reliable, efficient process to treat wastewaters and is often operated at temperatures exceeding 30 • C, involving energy consumption of biogas in temperate regions, where wastewaters are often discharged at variable temperatures generally below 20 • C. High-rate ambient temperature AD, without temperature control, is an economically attractive alternative that has been proven to be feasible at laboratory-scale. In this study, an ambient temperature pilot scale anaerobic reactor (2 m 3 ) was employed to treat real dairy wastewater in situ at a milk processing plant, at organic loading rates of 1.3 ± 0.6 to 10.6 ± 3.7 kg COD/m 3 /day and hydraulic retention times (HRT) ranging from 36 to 6 h. Consistent high levels of COD removal efficiencies, ranging from 50 to 70% for total COD removal and 70 to 84% for soluble COD removal, were achieved during the trial. Within the reactor biomass, stable active archaeal populations were observed, consisting mainly of Methanothrix (previously Methanosaeta) species, which represented up to 47% of the relative abundant active species in the reactor. The decrease in HRT, combined with increases in the loading rate had a clear effect on shaping the structure and composition of the bacterial fraction of the microbial community, however, without affecting reactor performance. On the other hand, perturbances in influent pH had a strong impact, especially when pH went higher than 8.5, inducing shifts in the microbial community composition and, in some cases, affecting negatively the performance of the reactor in terms of COD removal and biogas methane content. For example, the main pH shock led to a drop in the methane content to 15%, COD removals decreased to 0%, while the archaeal population decreased to ∼11% both at DNA and cDNA levels. Functional redundancy in the microbial community underpinned stable reactor performance and rapid reactor recovery after perturbations.

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“…While acetate was detected in both day 65 and day 120 samples, OTUs related to the genus Methanosaeta (methanogens that can grow solely on acetate) (12,49) were detected in very low proportions (Ͻ0.5%). Methanosaeta has high affinity for acetate (55) but has often been described to be less competitive at high acetate concentrations (31), although a recent study found strong competitiveness of these methanogens in animal wastewater with an elevated acetate level (59).…”

Section: Discussionmentioning

confidence: 99%

Targeting Bacteria and Methanogens To Understand the Role of Residual Slurry as an Inoculant in Stored Liquid Dairy Manure

Habtewold

Sokolov

VanderZaag

et al. 2018

Appl Environ Microbiol

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Microbial communities in residual slurry left after removal of stored liquid dairy manure have been presumed to increase methane emission during new storage, but these microbes have not been studied. While actual manure storage tanks are filled gradually, pilot- and farm-scale studies on methane emissions from such systems often use a batch approach. In this study, six pilot-scale outdoor storage tanks with (10% and 20%) and without residual slurry were filled (gradually or in batch) with fresh dairy manure, and methane and methanogenic and bacterial communities were studied during 120 days of storage. Regardless of filling type, increased residual slurry levels resulted in higher abundance of methanogens and bacteria after 65 days of storage. However, stronger correlation between methanogen abundance and methane flux was observed in gradually filled tanks. Despite some variations in the diversity of methanogens or bacteria with the presence of residual slurry, core phylotypes were not impacted. In all samples, the phylum Firmicutes predominated (∼57 to 70%) bacteria: >90% were members of Clostridia . Methanocorpusculum dominated (∼57 to 88%) archaeal phylotypes, while Methanosarcina gradually increased with storage time. During peak flux of methane, Methanosarcina was the major player in methane production. The results suggest that increased levels of residual slurry have little impact on the dominant methanogenic or bacterial phylotypes, but large population sizes of these organisms may result in increased methane flux during the initial phases of storage. IMPORTANCE Methane is the major greenhouse gas emitted from stored liquid dairy manure. Residual slurry left after removal of stored manure from tanks has been implicated in increasing methane emissions in new storages, and well-adapted microbial communities in it are the drivers of the increase. Linking methane flux to the abundance, diversity, and activity of microbial communities in stored slurries with different levels of residual slurry can help to improve the mitigation strategy. Mesoscale and lab-scale studies conducted so far on methane flux from manure storage systems used batch-filled tanks, while the actual condition in many farms involves gradual filling. Hence, this study provides important information toward determining levels of residual slurry that result in significant reduction of well-adapted microbial communities prior to storage, thereby reducing methane emissions from manure storage tanks filled under farm conditions.

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Unexpected competitiveness of Methanosaeta populations at elevated acetate concentrations in methanogenic treatment of animal wastewater

Cited by 39 publications

References 37 publications

Low carbon renewable natural gas production from coalbeds and implications for carbon capture and storage

Low carbon renewable natural gas production from coalbeds and implications for carbon capture and storage

Microbial Community Redundancy and Resilience Underpins High-Rate Anaerobic Treatment of Dairy-Processing Wastewater at Ambient Temperatures

Targeting Bacteria and Methanogens To Understand the Role of Residual Slurry as an Inoculant in Stored Liquid Dairy Manure

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