Unexpected field observations and transmission dynamics of contagious caprine pleuropneumonia in a sand gazelle herd

Lignereux, Louis; Chaber, Anne‐Lise; Saegerman, Claude; Manso-Silván, Lucía; Peyraud, Armelle; Apolloni, Andrea; Thiaucourt, François

doi:10.1016/j.prevetmed.2018.06.002

Cited by 18 publications

(38 citation statements)

References 24 publications

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“…Subsequently, Mccp has been isolated in other countries, such as Chad, Ethiopia, India, Oman, Sudan, Tunisia, Turkey, and Uganda [3]. CCPP is also now known to affect some species of exotic ungulates, particularly wildlife species, such as gazelles and gerenuks [4,5]. e disease is highly contagious and is transmitted by close contact through inhalation of respiratory droplets [2].…”

Section: Introductionmentioning

confidence: 99%

Development and Evaluation of an Immuno-Capture Enzyme-Linked Immunosorbent Assay to Quantify the Mycoplasma capricolum subsp. capripneumoniae (Mccp) Protein in Contagious Caprine Pleuropneumonia (CCPP) Vaccine

Baziki

Charles

Nwankpa

et al. 2020

Veterinary Medicine International

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An effective contagious caprine pleuropneumonia (CCPP) vaccine is essential for the increased production of healthy goats in a cost-effective manner and the prevention of animal-to-animal transmission for both domestic animals and wildlife. Quality control of this vaccine ensures that a reliable supply of pure, safe, and potent batches is obtained. As part of this control, in vitro quantification of Mycoplasma capricolum subsp. capripneumoniae (Mccp) protein in the final vaccines is required before the CCPP vaccine undergoes batch release and certification. The current method used for quantification is based on the measurement of total protein using the bicinchonic acid (BCA) test. This method quantifies the total amount of protein in the vaccine including contaminant protein from media, which can lead to overestimation of the quantity of Mccp protein, resulting in reduced vaccine immunogenicity. An immuno-capture ELISA (ICE) was developed for specific detection and quantification of the Mccp antigen in the CCPP vaccine. As the ICE detects and measures the amount of antigen between two layers of antibodies, capture and detecting antibodies are required. Mouse monoclonal antibodies (mAbs) that detect the Mccp antigen were produced and characterized. One of these mAbs, Mccp-25, was used to develop the ICE as an unlabelled capture antibody and horseradish peroxidase conjugated detecting antibody. The ICE was standardized and evaluated using an internal reference sample, experimental CCPP vaccines and commercial CCPP vaccines. A comparison between the polymerase chain reaction (PCR) and ICE showed good correlation between the two assays. Also, an in vitro ICE method correlated well with an in vivo sero-conversion in goats that were vaccinated with selected test vaccines. The sensitivity of the ICE was estimated at 30 ng/ml.

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Section: Introductionmentioning

confidence: 99%

Development and Evaluation of an Immuno-Capture Enzyme-Linked Immunosorbent Assay to Quantify the Mycoplasma capricolum subsp. capripneumoniae (Mccp) Protein in Contagious Caprine Pleuropneumonia (CCPP) Vaccine

Baziki

Charles

Nwankpa

et al. 2020

Veterinary Medicine International

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“…A set of 34 Mccp strains was chosen from the CIRAD-ASTRE bank ( Table 1 ). It included strains originating from the various regions where CCPP is known to be present and, notably, five strains isolated from wildlife (wild goat from Qatar ( Capra aegagrus ) ( Arif et al., 2007 ), and Arabian sand gazelle ( Gazella marica ) ( Lignereux et al., 2018 ), Arabian oryx ( Oryx leucoryx ) ( Chaber et al., 2014 ) and scimitar-horned oryx ( Oryx dammah ) from the United Arab Emirates. It also included six strains isolated in various locations in Tanzania in 2013, a year of high CCPP prevalence (E. Noah, personal communication).…”

Section: Methodsmentioning

confidence: 99%

A whole-genome worldwide molecular epidemiology approach for contagious caprine pleuropneumonia

Loire

Ibrahim

Manso-Silván

et al. 2020

Heliyon

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Contagious caprine pleuropneumonia is an infectious and contagious disease affecting goats and wildlife ruminants, mostly in Africa and Asia. It is caused by a mycoplasma, Mycoplasma capricolum susbp. capripneumoniae , which is very fastidious. This may be the reason why there are few reports of its isolation and characterization. This study describes the development of a whole genome typing strategy based on sequencing reads assemblies on a reference genome (Abomsa, GenBank accession LM995445) and extraction of informative single nucleotide polymorphism. FASTA sequences inferred from the variant calling files were used to establish a comprehensive phylogenetic tree based on 2880 SNPs. This tree included a total of 34 strains originating from all the regions where CCPP has been detected, as well as strains isolated from wildlife. A recent isolate from West-Niger was positioned closely to another 1995 East-Niger isolate, an indication that CCPP may be extending westward in Africa. Six 2013 Tanzanian isolates had identical sequences in spite of diverse geographical origins. This could be explained by the clonal expansion of a virulent strain at that time in East Africa. Although all strains isolated from wildlife in the Middle East were in the same phylogenetic group, this may not sign an adaptation to new hosts. The most probable explanation for wildlife contamination remains the contact with goats. This strategy will easily accommodate new data in the near future and should become a gold-standard high-resolution typing procedure for the surveillance of contagious caprine pleuropneumonia.

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“…The duration of the infectious period has been estimated at about 19 days by an in silico model (Lignereux et al., ). However, from a practical point of view, two phases of the disease should be considered: The acute stage where excretion is maximum, and when the lungs are filled with exudate containing very high concentrations of Mccp (10 9 /mL).…”

Section: Assessmentmentioning

confidence: 99%

“…To summarise the routes of transmission based on their importance: Direct contact is the main source of contamination/transmission (OIE, ) Some cases of distant spread have been speculated to occur by airborne transmission (Lignereux et al., ) Vectors are not considered to play any role in the transmission of CCPP Fomites are not considered to play any role in the transmission of CCPP Animal products do not play any role in the transmission of CCPP Environmental contamination is considered negligible due to the fragility of mycoplasmas …”

Section: Assessmentmentioning

confidence: 99%

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Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): contagious caprine pleuropneumonia

More¹,

Bøtner²,

Butterworth³

et al. 2017

EFS2

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Contagious caprine pleuropneumonia has been assessed according to the criteria of the Animal Health Law (AHL), in particular criteria of Article 7 on disease profile and impacts, Article 5 on the eligibility of contagious caprine pleuropneumonia to be listed, Article 9 for the categorisation of contagious caprine pleuropneumonia according to disease prevention and control rules as in Annex IV and Article 8 on the list of animal species related to contagious caprine pleuropneumonia. The assessment has been performed following a methodology composed of information collection and compilation, expert judgement on each criterion at individual and, if no consensus was reached before, also at collective level. The output is composed of the categorical answer, and for the questions where no consensus was reached, the different supporting views are reported. Details on the methodology used for this assessment are explained in a separate opinion. According to the assessment performed, contagious caprine pleuropneumonia can be considered eligible to be listed for Union intervention as laid down in Article 5(3) of the AHL. The disease would comply with the criteria as in Sections 4 and 5 of Annex IV of the AHL, for the application of the disease prevention and control rules referred to in points (d) and (e) of Article 9(1). The assessment here performed on compliance with the criteria as in Section 1 of Annex IV referred to in point (a) of Article 9(1) is inconclusive. The animal species to be listed for contagious caprine pleuropneumonia according to Article 8(3) criteria are goats and other species of the family Bovidae as susceptible.

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Unexpected field observations and transmission dynamics of contagious caprine pleuropneumonia in a sand gazelle herd

Cited by 18 publications

References 24 publications

Development and Evaluation of an Immuno-Capture Enzyme-Linked Immunosorbent Assay to Quantify the Mycoplasma capricolum subsp. capripneumoniae (Mccp) Protein in Contagious Caprine Pleuropneumonia (CCPP) Vaccine

Development and Evaluation of an Immuno-Capture Enzyme-Linked Immunosorbent Assay to Quantify the Mycoplasma capricolum subsp. capripneumoniae (Mccp) Protein in Contagious Caprine Pleuropneumonia (CCPP) Vaccine

A whole-genome worldwide molecular epidemiology approach for contagious caprine pleuropneumonia

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): contagious caprine pleuropneumonia

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