Unexpected genetic diversity of Mycoplasma agalactiae caprine isolates from an endemic geographically restricted area of Spain

Fe, Christian de la; Amores, J.; Tardy, Florence; Sagné, Eveline; Nouvel, Laurent-Xavier; Citti, Christine

doi:10.1186/1746-6148-8-146

Cited by 19 publications

(17 citation statements)

References 28 publications

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“…Despite the variability found, the data also suggested the presence of two main groups formed by the Movp strains taken from sheep and goats. Recently, molecular typing of sheep and goat Ma strains with different epidemiological characteristics has revealed different levels of genetic variability between the two hosts (De la Fe and others 2012, Ariza-Miguel and others 2013). It is yet to be determined whether a high degree of variability might have phenotypical implications and practical consequences.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of phenotypic and genotypic profiles among caprine and ovineMycoplasma ovipneumoniaestrains

Maksimović

Amores

et al. 2017

Veterinary Record

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() is considered to be one of the most important mycoplasmas causing respiratory disease in small ruminants. Most epidemiologic and characterisation studies have been conducted on strains collected from sheep. Information on the presence and characteristics of in healthy and pneumonic goats is limited. Phenotypic or genotypic differences between sheep and goat isolates have never been studied. The objective of our study was to characterise and compare the similarities and differences between caprine and ovine strains isolated from affected and asymptomatic animals in order to elucidate phenotypic and genotypic variability. Four different techniques were used on a set of 23 isolates. These included SDS-PAGE, Western blotting, random amplified polymorphic DNA and the heat shock protein 70 gene sequence-based method. A high degree of phenotypic and genotypic heterogeneity among strains was demonstrated in this study. Our results demonstrated differences between goat and sheep strains, revealing not only a link between strains and host ruminant species, but by geographical origin as well. However, the finding of immunodominant antigens of molecular masses 36, 38, 40 and 70 kDa (±3 kDa) in isolates from sheep and goats foretells their potential use in the development of serological diagnostic tests and vaccines.

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Section: Discussionmentioning

confidence: 99%

“…The presence of single or multiple Movp strains during the outbreaks in goat herds has not been studied. The existence of phenotypic or genotypic differences between sheep and goat isolates, as has been recently considered for Mycoplasma agalactiae ( Ma ) (De la Fe and others 2012, Ariza-Miguel and others 2013), has not been determined.…”

Section: Introductionmentioning

confidence: 99%

Comparison of phenotypic and genotypic profiles among caprine and ovineMycoplasma ovipneumoniaestrains

Maksimović

Amores

et al. 2017

Veterinary Record

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“…To obtain further information about the endemic clone, a representative isolate, namely, 1668a, was fully sequenced, and future studies will help to clarify the molecular mechanisms involved in the evolutionary success of this clone as well as to provide new insights into the genomic diversity and evolution of the species. In contrast, several studies have reported an unexpectedly high diversity in M. agalactiae Spanish isolates recovered from goats (3,4,14). Further studies are necessary to test whether this is because various CA-causing mycoplasmas have been detected in Spanish goat herds (15)(16)(17), while M. agalactiae has been the only species detected in sheep, limiting the possibility of genetic exchange (11).…”

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confidence: 94%

Molecular Characterization of Mycoplasma agalactiae Reveals the Presence of an Endemic Clone in Spain

2013

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Mycoplasma agalactiae isolates from Spain were genetically characterized to investigate their genomic diversity and to better understand their relationship to isolates from other countries. Molecular typing revealed a high genomic homogeneity in Spanish M. agalactiae isolates, which clearly shows the circulation of one endemic clonal population. Mycoplasma agalactiae is the main etiologic agent of contagious agalactia (CA), a serious syndrome affecting small ruminants; the World Organization for Animal Health must be notified of its occurrence because of its high economic significance worldwide. The first genomic studies showed little genomic diversity within M. agalactiae species, apart from that provided by antigenic variation (1, 2). Recently, the development of new sequence-based typing systems has revealed more genetic heterogeneity than previously thought (3-5). To investigate the genomic diversity of Spanish M. agalactiae isolates and to elucidate their relationship to those from other geographic areas, we analyzed isolates from Spain using pulsed-field gel electrophoresis (PFGE), which has been demonstrated to be robust and discriminative for typing different species of mycoplasmas (6-9), including M. agalactiae (3, 10); we also used the most recently developed sequence-based typing techniques, such as multilocus variable-

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“…So, PCR methods have been developed for M. agalactiae (Tola et al, 1997;Marenda et al, 2005;De la Fe et al, 2012), for Mycoides spp. cluster (Manso-Silvan et al, 2007;Woubit et al, 2007) and for M. putrefaciens (Peyraud et al, 2003), although PCR inhibitors in milk could diminish the usefulness of this procedure (Becker et al, 2012).…”

Section: Mycoplasma Sppmentioning

confidence: 99%

Milk hygiene in small ruminants: A review

Gonzalo

2018

Span. j. agric. res.

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Somatic cell count (SCC), mammary pathogens prevalence, total and specific bacterial counts, antimicrobial residues, macroscopic sediment, water addition, aflatoxins and other contaminants constitute the basis for milk payment-schemes, monitoring and improvement of flock hygiene and health management, and development of analytical surveillance programs in the dairy small ruminants. The present work reviews factors influencing the variation of these variables, including milk analytical methods, storage and preservation, along with management implications during the last two decades. Following farmer and cooperative educational programs, progressive reductions have been reported for total bacterial count and antimicrobial residue occurrence in bulk tank milk. These results were consistent, however, with high values for SCC and specific bacterial populations. Thus, mastitis control programs should be intensified to increase hygiene in milk and economic returns for producers and processors. In addition, the implementation of programs to reduce specific bacterial counts (i.e., psychrotrophs, coliforms, Clostridium spp. spores) and mammary pathogen prevalence (i.e., Staph. aureus, Mycoplasma spp.), as well as the use of combined screening methods for an increased rate of antimicrobial detection, are currently required strategies which are positively valuated by milk processors, industry and consumers. Other contaminants may also be present, but cost-effective screening and analytical systems have not yet been implemented. This review aims to be helpful for troubleshooting milk quality and safety, developing future premium payment systems and industry quality-standards, optimizing management, on-farm risk traceability systems and consumer acceptance.

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Unexpected genetic diversity of Mycoplasma agalactiae caprine isolates from an endemic geographically restricted area of Spain

Cited by 19 publications

References 28 publications

Comparison of phenotypic and genotypic profiles among caprine and ovineMycoplasma ovipneumoniaestrains

Comparison of phenotypic and genotypic profiles among caprine and ovineMycoplasma ovipneumoniaestrains

Molecular Characterization of Mycoplasma agalactiae Reveals the Presence of an Endemic Clone in Spain

Milk hygiene in small ruminants: A review

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