2013
DOI: 10.1016/j.bpj.2013.01.051
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Unexpected Membrane Dynamics Unveiled by Membrane Nanotube Extrusion

Abstract: In cell mechanics, distinguishing the respective roles of the plasma membrane and of the cytoskeleton is a challenge. The difference in the behavior of cellular and pure lipid membranes is usually attributed to the presence of the cytoskeleton as explored by membrane nanotube extrusion. Here we revisit this prevalent picture by unveiling unexpected force responses of plasma membrane spheres devoid of cytoskeleton and synthetic liposomes. We show that a tiny variation in the content of synthetic membranes does … Show more

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Cited by 70 publications
(77 citation statements)
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“…As shown in Table S1, the specific capacitance values of caseincontaining DPhPC GUVs and conventional DPhPC GUVs measured using standard glass patch pipettes or pipettes coated with a novolac resin were all within experimental uncertainty of the published value for solvent-free DPhPC (29). In contrast, GUVs prepared from an oil/DPhPC mixture using the inverted emulsion technique had a lower specific capacitance, confirming they retain some oil, which would thicken the membrane and also account for their unusual mechanical properties (30). However, based on the specific capacitance (Fig.…”
Section: Resultsmentioning
confidence: 70%
“…As shown in Table S1, the specific capacitance values of caseincontaining DPhPC GUVs and conventional DPhPC GUVs measured using standard glass patch pipettes or pipettes coated with a novolac resin were all within experimental uncertainty of the published value for solvent-free DPhPC (29). In contrast, GUVs prepared from an oil/DPhPC mixture using the inverted emulsion technique had a lower specific capacitance, confirming they retain some oil, which would thicken the membrane and also account for their unusual mechanical properties (30). However, based on the specific capacitance (Fig.…”
Section: Resultsmentioning
confidence: 70%
“…However, the single exponential function used in these works does not provide a good fit to the force relaxation data in our experiments on axonal membrane. There has been an attempt in multicomponent synthetic vesicle systems by Campillo et al at fitting a similar force relaxation behavior to two well-separated relaxation timescales (18). They conjectured that the fast timescale is an effect of the intermonolayer friction (as theoretically formulated in (19)) whereas the slower timescale comes from an unknown diffusive process over the length of the tether.…”
Section: Force Relaxation After Step-elongation Of a Tethermentioning
confidence: 98%
“…Indeed, for some cell types, it seems that measured tube forces reflect almost only the force needed to detach the membrane from the cytoskeleton, while in other cases, tube forces seem to reflect the real in-plane membrane tension (17,73,253). Moreover, the complex composition of the membrane in a cell, with transmembrane proteins that prevent the two lipid leaflets from sliding over each other, creates an additional friction force that places membrane tubes out of equilibrium (50). The take-home message concerning membrane/cortical tension is that while micropipette aspiration can directly measure cortical tension, it is difficult to quantify how much of this tension derives from the membrane as opposed to the cytoskeleton, unless the membrane detaches from its underlying cytoskeleton, allowing for both modules to be analyzed separately (49).…”
Section: Acto-myosin Cortex Mechanicsmentioning
confidence: 99%