Unexpected prevalence of parasite 18S rDNA sequences in winter among Antarctic marine protists

Cleary, Alison C.; Durbin, Edward G.

doi:10.1093/plankt/fbw005

Cited by 49 publications

(54 citation statements)

References 52 publications

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“…Parasitic Syndiniales sequences are commonly detected in 18S rRNA gene sequencing in high abundances (e.g., Sauvadet et al ; de Vargas et al ; Cleary and Durbin ; Gutierrez‐Rodriguez et al ). They affect a wide variety of hosts, ranging from diatoms (Berdjeb et al .…”

Section: Resultsmentioning

confidence: 99%

“…Parasitic Syndiniales sequences are commonly detected in 18S rRNA gene sequencing in high abundances (e.g., Sauvadet et al 2010;de Vargas et al 2015;Cleary and Durbin 2016;Gutierrez-Rodriguez et al 2019). They affect a wide variety of hosts, ranging from diatoms (Berdjeb et al 2018), tintinnides (Harada et al 2007), and radiolarians (Dolven et al 2007) to copepods (Skovgaard et al 2005).…”

Section: Flagellatesmentioning

confidence: 99%

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Eukaryotic influence on the oceanic biological carbon pump in the Scotia Sea as revealed by 18S rRNA gene sequencing of suspended and sinking particles

Duret

Lampitt

Lam

2019

Limnology & Oceanography

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Suspended marine particles constitute most of the particulate organic matter pool in the oceans, thereby providing substantial substrates for heterotrophs, especially in the mesopelagic. Conversely, sinking particles are major contributors to carbon fluxes defining the strength of the biological carbon pump (BCP). This study is the first to investigate the differential influence of eukaryotic communities to suspended and sinking particles, using 18S rRNA gene sequencing on particles collected with a marine snow catcher in the mixed layer and upper mesopelagic of the Scotia Sea, Southern Ocean. In the upper mesopelagic, most eukaryotic phytoplankton sequences belonged to chain‐forming diatoms in sinking particles and to prymnesiophytes in suspended particles. This suggests that diatom‐enriched particles are more efficient in carbon transfer to the upper mesopelagic than those enriched in prymnesiophytes in the Scotia Sea, the latter more easily disintegrating into suspended particles. In the upper mesopelagic, copepods appeared most influential on sinking particles whereas soft‐tissue metazoan sequences contributed more to suspended particles. Heterotrophic protists and fungi communities were distinct between mixed layer and upper mesopelagic, implying that few protists ride along sinking particles. Furthermore, differences between predatory flagellates and radiolarians between suspended and sinking particles implied different ecological conditions between the two particles pools, and roles in the BCP. Molecular analyses of sinking and suspended particles constitute powerful diagnostic tools to study the eukaryotic influence on the BCP in a more holistic manner compared to classic carbon export studies focusing on sinking particles.

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Section: Resultsmentioning

confidence: 99%

Section: Flagellatesmentioning

confidence: 99%

Eukaryotic influence on the oceanic biological carbon pump in the Scotia Sea as revealed by 18S rRNA gene sequencing of suspended and sinking particles

Duret

Lampitt

Lam

2019

Limnology & Oceanography

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“…Based on the metabarcoding of environmental 18S rRNA genes retrieved from various marine habitats, MALV-I was ubiquitous but preferred surface waters regardless of coast and ocean (Groisillier, Massana, Valentin, Vaulot, & Guillou, 2006). As for MALV-II, all OTUs belonged to the dinoflagellates Amoebophyra and Syndinium (Figure 5a), which were recognized as parasites that infect protozoans, such as radiolarians and dinoflagellates (Cleary & Durbin, 2016;Groisillier et al, 2006;Guillou et al, 2008). The appearance of MALV-II implied that host-parasite interactions might often occur in this region in winter.…”

Section: Discussionmentioning

confidence: 99%

Spatial variation of abundant picoeukaryotes in the subtropical Kuroshio Current in winter

et al. 2020

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To understand the importance of picoeukaryotes in the biogeochemical cycle in the subtropical Kuroshio Current, a year‐round survey of the hydrography and the distribution of picoeukaryotes were conducted in four oceanographic cruises from October 2012 to July 2013. In comparison with other seasons, the highest abundancy of photosynthetic picoeukaryotes, with concentrations >104 cells/ml, was observed around the eastern boundary of the Kuroshio in the winter. Accordingly, the composition of picoeukaryotes in this cold season was further studied by a metabarcoding analysis of the 18S rRNA gene. The majority of picoeukaryotes comprised Alveolata, followed by Haptophyta and Stramenopiles. Their composition was diverse in the waters affected by the Kuroshio and in the offshore province. For Haptophyta, in contrast to clade A prevailing in the Kuroshio waters, clade B1, which was considered the host of uncultivated diazotrophic cyanobacterium group A (UCYN‐A), appeared only in the offshore area. Similarly, in Stramenopiles, Pseudo‐nitzschia spp. and MAST‐1D, respectively, dominated in the Kuroshio‐influenced and offshore areas. While Alveolata was the most abundant group, the distributions of all lineages were similar. The association between picoeukaryote succession and hydrographic change is yet to be fully understood. Our results will assist future studies on the community composition of picoplankton and their relationship with marine ecology in the region.

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“…A few consensuses have been reached for planktonic protists, such as the dominance of parasite groups within Alveolata (Guillou et al, ; de Vargas et al, ). Moreover, it is well recognized that benthic protists are significantly different from planktonic groups (Chen, Pan, Yu, Yang, & Zhang, ; Cleary & Durbin, ; Coolen & Shtereva, ; Epstein & López‐García, ; Massana et al, ) and can even exhibit higher diversity than planktonic taxa (Chen et al, ; Forster et al, ). Furthermore, deep‐sea protists are much less studied (Pawlowski et al, ) relative to protists in coastal and shallow‐sea sediments (e.g., Gong et al, ; Massana et al, ; Chen et al, ).…”

Section: Introductionmentioning

confidence: 99%

Protist diversity and community assembly in surface sediments of the South China Sea

Huang

2019

MicrobiologyOpen

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Protists are pivotal components of marine ecosystems in terms of their high diversity, but protist communities have been poorly explored in benthic environments. Here, we investigated protist diversity and community assembly in surface sediments in the South China Sea (SCS) at a basin scale. Pyrosequencing of 18S rDNA was performed for a total of six samples taken from the surface seafloor at water depths ranging from 79 to 2,939 m. We found that Cercozoa was the dominant group, accounting for an average of 39.9% and 25.3% of the reads and operational taxonomic units (OTUs), respectively. The Cercozoa taxa were highly diverse, comprising 14 phylogenetic clades, six of which were affiliated with unknown groups belonging to Filosa and Endomyxa. Fungi were also an important group in both read‐ (18.1% on average) and OTU‐derived (9.3% on average) results. Moreover, the turnover patterns of the protist communities were differently explained by species sorting (53.3%), dispersal limitation (33.3%), mass effects (0%), and drift (13.3%). In summary, our findings show that the basin‐wide protist communities in the surface sediments of the SCS are primarily dominated by Cercozoa and are mainly assembled by species sorting and dispersal limitation.

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Unexpected prevalence of parasite 18S rDNA sequences in winter among Antarctic marine protists

Cited by 49 publications

References 52 publications

Eukaryotic influence on the oceanic biological carbon pump in the Scotia Sea as revealed by 18S rRNA gene sequencing of suspended and sinking particles

Eukaryotic influence on the oceanic biological carbon pump in the Scotia Sea as revealed by 18S rRNA gene sequencing of suspended and sinking particles

Spatial variation of abundant picoeukaryotes in the subtropical Kuroshio Current in winter

Protist diversity and community assembly in surface sediments of the South China Sea

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