Unfermented β-fructan Fibers Fuel Inflammation in Select Inflammatory Bowel Disease Patients

Armstrong, Heather; Bording-Jorgensen, Michael; Santer, Deanna M.; Zhang, Zhengxiao; Valcheva, Rosica; Rieger, Aja M.; Kim, Justin Sung-Ho; Dijk, Stephanie; Mahmood, Ramsha; Ogungbola, Olamide; Jovel, Juan; Moreau, F; Gorman, Hayley; Dickner, R; Jerasi, J; Mander, I K; Lafleur, Dawson; Cheng, Chu; Petrova, A; Jeanson, Terri-Lyn; Mason, Andrew L.; Sergi, Consolato; Levine, Arie; Chadee, Kris; Armstrong, David M.; Rauscher, Sarah; Bernstein, Çharles N.; Carroll, Matthew; Huynh, Hien Q.; Walter, Jens; Madsen, Karen; Dieleman, Levinus A.; Wine, Eytan

doi:10.1053/j.gastro.2022.09.034

Cited by 91 publications

(72 citation statements)

References 39 publications

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“…However, not all fibers are well tolerated, as sensitivity and detrimental effects have been reported mostly by patients with inflammatory bowel diseases (IBD) [ 25 , 26 ]. A recent study indicated that such negative effects can, in part, be explained by the lack of microbiota fermentative activities in some of IBD patients, resulting in the presence of unfermented β-fructan fibers that can activate pro-inflammatory responses in the gut [ 27 ]. This places bacterial fermentation as a crucial step to achieve the benefits of fiber consumption.…”

Section: Introductionmentioning

confidence: 99%

“…This places bacterial fermentation as a crucial step to achieve the benefits of fiber consumption. However, as alluded above, soluble fibers have also shown to have direct effects on intestinal epithelial cells [ 27 – 30 ], even possibly through mechanisms that are independent of the gut microbiota [ 31 ]. Thus, unraveling microbiota-dependent versus independent roles of soluble fibers is important to understand their mechanisms.…”

Section: Introductionmentioning

confidence: 99%

“…In vitro and in vivo studies indicated that inulin improves the epithelial barrier, an effect associated with increased expression of tight and adherens junction genes [ 28 , 29 , 39 , 45 – 48 ]. Interestingly, inulin originated from the same plant source (chicory root) but with distinct levels of purity and polymerization presented different effects on intestinal barrier, indicating that these physicochemical aspects are relevant for the biological effects of this fiber [ 15 , 27 ]. Modulation of inflammatory cytokines [ 45 , 46 ] and increased production of mucus [ 49 ] and defensins [ 18 , 39 , 45 , 46 ] have also been reported in the literature, which collectively demonstrates that inulin has significant effects on different aspects of the intestinal epithelium.…”

Section: Introductionmentioning

confidence: 99%

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Inulin diet uncovers complex diet-microbiota-immune cell interactions remodeling the gut epithelium

et al. 2023

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Background The continuous proliferation of intestinal stem cells followed by their tightly regulated differentiation to epithelial cells is essential for the maintenance of the gut epithelial barrier and its functions. How these processes are tuned by diet and gut microbiome is an important, but poorly understood question. Dietary soluble fibers, such as inulin, are known for their ability to impact the gut bacterial community and gut epithelium, and their consumption has been usually associated with health improvement in mice and humans. In this study, we tested the hypothesis that inulin consumption modifies the composition of colonic bacteria and this impacts intestinal stem cells functions, thus affecting the epithelial structure. Methods Mice were fed with a diet containing 5% of the insoluble fiber cellulose or the same diet enriched with an additional 10% of inulin. Using a combination of histochemistry, host cell transcriptomics, 16S microbiome analysis, germ-free, gnotobiotic, and genetically modified mouse models, we analyzed the impact of inulin intake on the colonic epithelium, intestinal bacteria, and the local immune compartment. Results We show that the consumption of inulin diet alters the colon epithelium by increasing the proliferation of intestinal stem cells, leading to deeper crypts and longer colons. This effect was dependent on the inulin-altered gut microbiota, as no modulations were observed in animals deprived of microbiota, nor in mice fed cellulose-enriched diets. We also describe the pivotal role of γδ T lymphocytes and IL-22 in this microenvironment, as the inulin diet failed to induce epithelium remodeling in mice lacking this T cell population or cytokine, highlighting their importance in the diet-microbiota-epithelium-immune system crosstalk. Conclusion This study indicates that the intake of inulin affects the activity of intestinal stem cells and drives a homeostatic remodeling of the colon epithelium, an effect that requires the gut microbiota, γδ T cells, and the presence of IL-22. Our study indicates complex cross kingdom and cross cell type interactions involved in the adaptation of the colon epithelium to the luminal environment in steady state.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inulin diet uncovers complex diet-microbiota-immune cell interactions remodeling the gut epithelium

et al. 2023

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“…While a high fiber diet may have some anti-inflammatory effects in animal models and small cohort studies of patients with IBD, the exact type and ideal amount of fiber to reduce inflammation and induce remission in patients with IBD is unknown. Armstrong et al [ 92 ] recently demonstrated that unfermented dietary β-fructan fibers can induce proinflammatory cytokines in a subset of IBD intestinal biopsies cultured ex vivo, and immune cells. The results suggest that some dietary fibers have detrimental effects in select patients with active IBD who lack fermentative microbe activities.…”

Section: Emerging Dietary Interventions In Ibdmentioning

confidence: 99%

Dietary Exposures and Interventions in Inflammatory Bowel Disease: Current Evidence and Emerging Concepts

et al. 2023

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Diet is intimately linked to the gastrointestinal (GI) tract and has potent effects on intestinal immune homeostasis. Inflammatory bowel disease (IBD) is characterized by chronic inflammation of the GI tract. The therapeutic implications of diet in patients with IBD have received significant attention in recent years. In this review, we provide a contemporary and comprehensive overview of dietary exposures and interventions in IBD. Epidemiological studies suggest that ultra-processed foods, food additives, and emulsifiers are associated with a higher incidence of IBD. Exclusion and elimination diets are associated with improved symptoms in patients with IBD, but no effects on objective markers of inflammation. Specific dietary interventions (e.g., Mediterranean, specific carbohydrate, high fiber, ketogenic, anti-inflammatory diets) have been shown to reduce symptoms, improve inflammatory biomarkers, and quality of life metrics to varying degrees, but these studies are limited by study design, underpowering, heterogeneity, and confounding. To date, there is no robust evidence that any dietary intervention alone may replace standard therapies in patients with IBD. However, diet may play an adjunct role to induce or maintain clinical remission with standard IBD therapies. The results of novel dietary trials in IBD such as personalized fiber, intermittent fasting, and time-restricted diets are eagerly awaited.

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“…This is because most people with IBD do not have the proper microorganisms to digest fiber. The researchers concluded that while fiber is beneficial for those with adequate levels of special microbes (the microbiome), some dietary fibers may be harmful to patients with active IBD who have lack the appropriate microbes to digest them [10].…”

Section: Introductionmentioning

confidence: 99%

Use of rice flour in wheat bread technology

Шевченко

Drobot

2022

Eureka: LS

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Last decade is characterized by the increase in the incidence of inflammatory bowel diseases all over the world. Diet therapy, which includes reducing the amount of dietary fiber and including phospholipids in it, is effective in maintaining a sTable condition in case of these diseases. A promising raw material with a low dietary fiber content is rice flour, which may be added to the recipe of bakery products to replace part of wheat flour. The aim of the work was to determine the influence of rice flour on the course of the technological process and the characteristics of the dough in bakery products manufacturing, as well as on the quality indicators of bread made from wheat flour, which contains lecithin. Rice flour contains 1.8 times less protein than wheat flour, but this protein is more complete in amino acid composition. The content of dietary fibers in rice flour is 8.5 times lower. The gas-forming capacity of the dough with lecithin separately and in a mixture with rice flour increases by 8.4−18.7 % when replacing 10−40 % of wheat flour. It was established that in the dough sample with lecithin, the amount of formed and fermented sugars increased by 1.2 % and 12.1 %, respectively, compared to the control sample without additives. With an increase in the percentage of replacement of wheat flour with rice flour, the amount of formed sugars increased by 35.2−39.0 %. The amount of fermented sugars also increased by 19.6−31.8 % with an increase in the percentage of replacement. The shape stability of bread slightly improved with the addition of lecithin. However, when adding rice flour, the shape stability of the products decreased by 7.1−26.8 %, as well as the specific volume and porosity of bread

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Unfermented β-fructan Fibers Fuel Inflammation in Select Inflammatory Bowel Disease Patients

Cited by 91 publications

References 39 publications

Inulin diet uncovers complex diet-microbiota-immune cell interactions remodeling the gut epithelium

Inulin diet uncovers complex diet-microbiota-immune cell interactions remodeling the gut epithelium

Dietary Exposures and Interventions in Inflammatory Bowel Disease: Current Evidence and Emerging Concepts

Use of rice flour in wheat bread technology

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