UniChromCD for Demultiplexing Time-Resolved Charge Detection-Mass Spectrometry Data

Sanders, James D.; Owen, October N.; Tran, Brian H.; Juetten, Kyle J.; Marty, Michael T.

doi:10.1021/acs.analchem.4c03250

Anal. Chem.

2024

DOI: 10.1021/acs.analchem.4c03250

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UniChromCD for Demultiplexing Time-Resolved Charge Detection-Mass Spectrometry Data

James D. Sanders,

October N. Owen,

Brian H. Tran

et al.

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Coupling Online Size Exclusion Chromatography with Charge Detection-Mass Spectrometry Using Hadamard Transform Multiplexing

Sanders,

Owen,

Tran

et al. 2024

Anal. Chem.

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Charge detection mass spectrometry (CD-MS) is a powerful technique for the analysis of large, heterogeneous biomolecules. By directly measuring the charge states of individual ions, CD-MS can measure the masses from spectra where conventional deconvolution approaches fail due to the lack of isotopic resolution or distinguishable charge states. However, CD-MS is inherently slow because hundreds or thousands of spectra need to be collected to produce adequate ion statistics. The slower speed of CD-MS complicates efforts to couple it with online separation techniques, which limit the number of spectra that can be acquired during a chromatographic peak. Here, we present the application of Hadamard transform multiplexing to online size exclusion chromatography (SEC) coupled with Orbitrap CD-MS, with a goal of using SEC for separating complex mixtures prior to CD-MS analysis. We developed a microcontroller to deliver pulsed injections from a large sample loop onto a SEC for online CD-MS analysis. Data showed a series of peaks spaced according to the pseudorandom injection sequence, which were demultiplexed with a Hadamard transform algorithm. The demultiplexed data revealed improved CD-MS signals while preserving retention time information. This multiplexing approach provides a general solution to the inherent incompatibilities of online separations and CD-MS detection that will enable a range of applications.

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Coupling Online Size Exclusion Chromatography with Charge Detection-Mass Spectrometry Using Hadamard Transform Multiplexing

Sanders,

Owen,

Tran

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

show abstract

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UniChromCD for Demultiplexing Time-Resolved Charge Detection-Mass Spectrometry Data

Cited by 1 publication

References 46 publications

Coupling Online Size Exclusion Chromatography with Charge Detection-Mass Spectrometry Using Hadamard Transform Multiplexing

Coupling Online Size Exclusion Chromatography with Charge Detection-Mass Spectrometry Using Hadamard Transform Multiplexing

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