Unidirectional particle transport in microfluidic chips operating in a tri-axial magnetic field for particle concentration and bio-analyte detection

Sadeghidelouei, Negar; Abedini-Nassab, Roozbeh

doi:10.1007/s10404-023-02702-y

Cited by 4 publications

(1 citation statement)

References 46 publications

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“…Microfluidic chips enhanced with active particle manipulation methods use at least one force other than hydrodynamic forces to manipulate cells. Methods based on magnetic [29][30][31][32][33][34], electric [35][36][37][38], acoustic [39][40][41][42][43], and optical forces [44][45][46][47][48], each of which, with their advantages and disadvantages, have been developed. In general, the active methods are rather more complicated than the passive methods; however, they typically offer more precise control of individual cells.…”

Section: Active Cell Manipulation Microfluidicsmentioning

confidence: 99%

Single-Cell RNA Sequencing in Organ and Cell Transplantation

Abedini-Nassab,

Taheri,

Emamgholizadeh

et al. 2024

Biosensors

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Single-cell RNA sequencing is a high-throughput novel method that provides transcriptional profiling of individual cells within biological samples. This method typically uses microfluidics systems to uncover the complex intercellular communication networks and biological pathways buried within highly heterogeneous cell populations in tissues. One important application of this technology sits in the fields of organ and stem cell transplantation, where complications such as graft rejection and other post-transplantation life-threatening issues may occur. In this review, we first focus on research in which single-cell RNA sequencing is used to study the transcriptional profile of transplanted tissues. This technology enables the analysis of the donor and recipient cells and identifies cell types and states associated with transplant complications and pathologies. We also review the use of single-cell RNA sequencing in stem cell implantation. This method enables studying the heterogeneity of normal and pathological stem cells and the heterogeneity in cell populations. With their remarkably rapid pace, the single-cell RNA sequencing methodologies will potentially result in breakthroughs in clinical transplantation in the coming years.

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Section: Active Cell Manipulation Microfluidicsmentioning

confidence: 99%

Single-Cell RNA Sequencing in Organ and Cell Transplantation

Abedini-Nassab,

Taheri,

Emamgholizadeh

et al. 2024

Biosensors

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On the coupling of immersed boundary and lattice Boltzmann method to study the dynamic behavior of a magnetic particle around an actuating magnetic disk in a fluid domain

Gerivani,

Nazari,

Abedini-Nassab

2024

Journal of Magnetism and Magnetic Materials

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Characterization of AI-enhanced magnetophoretic transistors operating in a tri-axial magnetic field for on-chip bioparticle sorting

Abedini-Nassab,

Adibi,

Ahmadiasl

2024

Sci Rep

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We demonstrate two general classes of magnetophoretic transistors, called the “trap” and the “repel-and-collect” transistors, capable of switching single magnetically labeled cells and magnetic particles between different paths in a microfluidic chamber. Compared with prior work on magnetophoretic transistors operating in a two-dimensional in-plane rotating field, the use of a tri-axial magnetic field has the fundamental advantages of preventing particle cluster formation and better syncing of single particles with the general operating clock. We use finite element methods to investigate the energy distribution on the chip surface and to predict the particle behavior at various device geometries. We then fabricate the proposed transistors and compare the experimental results with the simulation predictions. We found that with gate electrical currents of ~ 40 mA for a transistor with proper geometry, complete switching of magnetic particles with diameters in the range of 8–15 μm is achieved. We show that the device is reliable and works well at different magnetic field strengths (50–100 Oe) and frequencies (0.05–0.5 Hz). We also employed an image processing code with a trained convolutional neural network to automate the proposed transistors for identifying and sorting particles with various sizes and magnetic susceptibilities with accuracies higher than 98%. The proposed transistors can be used in designing novel magnetophoretic circuits for important applications in biomedical microdevices and single-cell biology. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-74761-2.

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Unidirectional particle transport in microfluidic chips operating in a tri-axial magnetic field for particle concentration and bio-analyte detection

Cited by 4 publications

References 46 publications

Single-Cell RNA Sequencing in Organ and Cell Transplantation

Single-Cell RNA Sequencing in Organ and Cell Transplantation

On the coupling of immersed boundary and lattice Boltzmann method to study the dynamic behavior of a magnetic particle around an actuating magnetic disk in a fluid domain

Characterization of AI-enhanced magnetophoretic transistors operating in a tri-axial magnetic field for on-chip bioparticle sorting

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