SummaryThis paper presents neural networks (NNs) adaptive controller for an uncertain fractional‐order nonlinear system in strict‐feedback form, subject to input saturation, unavailable states for measurement, and external disturbances. The fractional‐order adaptive laws are derived based only on the output tracking error thanks to the implementation of the strictly positive real (SPR) property, differently from the existing results in the literature of fractional‐order strict‐feedback systems where all system states are used in the adaptive laws. The proposed design approach addresses the nonaffine nature of the control input due to saturation nonlinearity by using the mean value theorem and follows a nonrecursive design by using a state transformation where the advantage is twofold. First, it eliminates the explosion complexity found in back‐stepping control‐based approaches design, and second, it reduces the approximators units and parameters in controller implementation. Furthermore, an observer is introduced to estimate the unavailable newly defined states, and then an output adaptive feedback control design is ensured. An NN is used to approximate the unknown ideal control law, and an auxiliary control term is appended to deal with saturation effect, unknown disturbance, and approximation errors. The tracking error is proved to converge asymptotically to a bounded set using the Lyapunov theory. Simulation results on three examples show the effectiveness of the proposed approach.