Unified Nomenclature for Genes Involved in Prokaryotic Aerobic Arsenite Oxidation

Lett, Marie‐Claire; Müller, Daniel; Lièvremont, Didier; Silver, Simón; Santini, Joanne M.

doi:10.1128/jb.06391-11

Cited by 96 publications

(62 citation statements)

References 15 publications

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“…Typically, rice can accumulate As from both polluted and unpolluted paddy soils, posing a potential health risk to populations with high rice consumption in China, Southern Asian and certain Latin American areas (Li et al, 2011;Zhu et al, 2014). Microbial mediated As metabolic processes play a major role in As cycling in the plant-soil-microbe system, including arsenite (As(III)) oxidation, arsenate (As(V)) respiration, As(V) reduction, and As(III) methylation (Lett et al, 2012). Recently, anaerobic As(III) oxidation was found in the haloalkaliphilic bacterium Alkalilimnicola ehrlichii strain MLHE-1 and the photosynthetic purple sulfur bacterium Ectothiorhodospira sp.…”

Section: Introductionmentioning

confidence: 99%

“…Arsenic metabolism genes mainly consist of four types: aio genes (As(III) oxidation, previously named as aox/aso/aro) (Lett et al, 2012), arr genes (As(V) respiration) (Malasarn et al, 2004), ars genes (As(V) reduction related) (Oremland and Stolz, 2005), and arsM genes (As(III) methylation) (Mestrot et al, 2011;Qin et al, 2006). These genes, and their corresponding proteins or their subunits (subtypes) are frequently used as molecular markers for microbial ecology studies (Lever, 2013;Malasarn et al, 2004;Silver and Phung, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Metagenomic analysis revealed highly diverse microbial arsenic metabolism genes in paddy soils with low-arsenic contents

Xiao

et al. 2016

Environmental Pollution

127

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a b s t r a c tMicrobe-mediated arsenic (As) metabolism plays a critical role in global As cycle, and As metabolism involves different types of genes encoding proteins facilitating its biotransformation and transportation processes. Here, we used metagenomic analysis based on high-throughput sequencing and constructed As metabolism protein databases to analyze As metabolism genes in five paddy soils with low-As contents. The results showed that highly diverse As metabolism genes were present in these paddy soils, with varied abundances and distribution for different types and subtypes of these genes. Arsenate reduction genes (ars) dominated in all soil samples, and significant correlation existed between the abundance of arr (arsenate respiration), aio (arsenite oxidation), and arsM (arsenite methylation) genes, indicating the co-existence and close-relation of different As resistance systems of microbes in wetland environments similar to these paddy soils after long-term evolution. Among all soil parameters, pH was an important factor controlling the distribution of As metabolism gene in five paddy soils (p ¼ 0.018). To the best of our knowledge, this is the first study using high-throughput sequencing and metagenomics approach in characterizing As metabolism genes in the five paddy soil, showing their great potential in As biotransformation, and therefore in mitigating arsenic risk to humans.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metagenomic analysis revealed highly diverse microbial arsenic metabolism genes in paddy soils with low-arsenic contents

Xiao

et al. 2016

Environmental Pollution

127

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“…According to the previous studies and unified nomenclature proposed by Lett et al (2012), these subunits are encoded by aioB (small Rieske subunit, previously known as aoxA/aroB/asoB) and aioA (large molybdopyterin subunit, previously known as aoxB/aroA/asoB) genes. Regulatory genes: aioS (previously aoxS) and aioR (previously aoxR), as well as cytochrome C gene (cytC) were identified in a majority of arsenite oxidizers.…”

Section: Introductionmentioning

confidence: 99%

Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance

Drewniak

Dziewit

Ciezkowska

et al. 2013

Journal of Biotechnology

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Plasmid pSinA of Sinorhizobium sp. M14 (Alphaproteobacteria) is the first described, natural, self-transferable plasmid harboring a complete set of genes for oxidation of arsenite. Removal of this plasmid from cells of the host strain caused the loss of resistance to arsenic and heavy metals (Cd, Co, Zn and Hg) and abolished the ability to grow on minimal salt medium supplemented with sodium arsenite as the sole energy source. Plasmid pSinA was introduced into other representatives of Alphaproteobacteria which resulted in acquisition of new abilities concerning arsenic resistance and oxidation, as well as heavy metals resistance. Microcosm experiments revealed that plasmid pSinA can also be transferred via conjugation into other indigenous bacteria from microbial community of As-contaminated soils, including representatives of Alpha-and Gammaproteobacteria. Analysis of "natural" transconjugants showed that pSinA is functional (expresses arsenite oxidase) and is stably maintained in their cells after approximately 60 generations of growth under nonselective conditions. This work clearly demonstrates that pSinA is a self-transferable, broad-host-range plasmid, which plays an important role in horizontal transfer of arsenic metabolism genes.

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“…Another previous study has reported that COD removal efficiencies ranged from 80% to 97% in the methanogenic reactors, indicating an effective biodegradation [15]. The NH 4 + -N concentrations of the effluents from the MP reactor systems were significantly higher than those of RW or AP, implying that most of the ammonium was generated in the MP reactor, which may be due to the mineralization of organic nitrogen. The total concentrations of Zn, Cu, Fe, Mn and Cd in MP were much lower than those in RW (Table 1).…”

Section: Physico-chemical Properties and Heavy Metals In Wastewatermentioning

confidence: 91%

“…The widespread As detoxification by As(V) reductase (ArsC) is a pathway for microbial As(V) reduction. As(III) oxidation is catalyzed by As(III) oxidases, which are encoded by aioA genes (As(III) oxidation, previously named as aox/aso/aro) [4]. Moreover, As(III) can be methylated by microbes into various organic As species [5], which are catalyzed by the As(III) methylation (arsM) genes.…”

Section: Introductionmentioning

confidence: 99%

Arsenic Transformation in Swine Wastewater with Low-Arsenic Content during Anaerobic Digestion

Zhai

Qin

Guo

et al. 2017

Water

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Abstract:In this study, the raw wastewater (RW), and effluents from the acidogenic phase (AP) and methanogenic phase (MP) in a swine wastewater treatment plant were collected to investigate the occurrence and transformation of arsenic (As), as well as the abundance of As metabolism genes during the anaerobic digestion (AD) process. The results showed that total concentrations of As generally decreased by 33-71% after AD. Further analysis showed that the As species of the dissolved fractions were present mainly as dimethylarsinic acid (DMA), with arsenite (As(III)) and arsenate (As(V)) as the minor species. Moreover, real-time PCR (qPCR) results showed that As metabolism genes (arsC, arsenate reduction gene; aioA, arsenite oxidation gene and arsM, arsenite methylation gene) were highly abundant, with arsM being predominant among the metabolism genes. This study provides reliable evidence on As biotransformation in swine wastewater treatment process, suggesting that AD could be a valuable treatment to mitigate the risk of As in wastewater.

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Unified Nomenclature for Genes Involved in Prokaryotic Aerobic Arsenite Oxidation

Cited by 96 publications

References 15 publications

Metagenomic analysis revealed highly diverse microbial arsenic metabolism genes in paddy soils with low-arsenic contents

Metagenomic analysis revealed highly diverse microbial arsenic metabolism genes in paddy soils with low-arsenic contents

Structural and functional genomics of plasmid pSinA of Sinorhizobium sp. M14 encoding genes for the arsenite oxidation and arsenic resistance

Arsenic Transformation in Swine Wastewater with Low-Arsenic Content during Anaerobic Digestion

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