Uniform illumination of optically dense NMR samples

Spinach – A software library for simulation of spin dynamics in large spin systems

“…3. The initial state and the detection state in all magnetic resonance experiments are simple collections of single-spin or two-spin (in exotic experiments such as PHIP [47] and CIDNP [48][49][50]) orders -the magnetization that strays too far from these narrow regions of the vast state space might never find its way back.…”

Blue News Update: BODIPY-GTP Binds to the Blue-Light Receptor YtvA While GTP Does Not

“…For this purpose we used a Fiber Coupled Light Source that allows a continuous irradiation of the sample with λ max  = 455 nm directly within the magnet (for details see Figure S6 and S7 in the supplementary material). To achieve an uniform illumination of the NMR samples the end of the optical fiber was modified as described by Kuprov et al [44]. Ligand detected experiments were performed with 20 µM YtvA plus a 10-fold excess of the appropriate ligand.…”

“…Shown is the illumination of YtvA (500 µM in PBS) at λ max  = 455 nm directly inside the NMR tube. To achieve a homogeneous illumination within the hole sample the end of the fiber was etched stepwise (12 steps, 3 mm per step) in a mixture containing 30% hydrofluoric acid and 20% sulfuric acid at 60°C as described earlier by Kuprov et al [44].…”

Sensitivity Enhancement in Solution NMR via Photochemically Induced Dynamic Nuclear Polarization

“…28 Additionally, stepwise-tapered optical fibers were found to be effective for optically dense samples with high dye concentrations. 29 Moreover, the time-resolved photo-CIDNP experiments using a pulsed laser can provide great enhancements by eliminating various cancellation and nuclear Overhauser effects, which take place in CW laser experiments. 30,31 The cancellation effect is observed when the nuclear spins of the escaped radicals do not experience fast enough paramagnetic relaxation.…”