Band filling effect, which is due to tuning the electron occupation number near the Fermi level, on the intrinsic magnetic damping parameter is demonstrated by employing high quality epitaxial Co1−xNix alloy films, in which the Fermi level and the density of states can be continuously tuned by varying the Ni concentration x. The intrinsic magnetic damping parameter, measured by timeresolved magneto-optical Kerr effect, changes weakly at small x and increases sharply for x > 0.80. The experimental results are well reproduced by the density functional theory calculation. More interestingly, the magnetic damping parameter and the density of states near the Fermi level share similar variation trends, demonstrating their correlation. The band filling effect in 3d magnetic transition metal alloys provides a way to tune the magnetic damping parameter as a key element in controlling of energy loss and speed of spintronic devices.