Unilateral Partial Limbal Stem Cell Deficiency: Contralateral Versus Ipsilateral Autologous Cultivated Limbal Epithelial Transplantation

Vazirani, Jayesh; Basu, Sayan; Kenia, Hemal; Ali, Hasnat; Kacham, Santhosh; Mariappan, Indumathi; Sangwan, Veena

doi:10.1016/j.ajo.2013.11.011

Cited by 41 publications

(34 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As they hold the possible risk of infection transfer, variable tissue quality, and limited transparency, alternative seeding scaffolds have been proposed [43] .…”

Section: Discussionmentioning

confidence: 99%

The potential therapeutic effect of stem cells loaded on two different vehicles (amniotic membrane and platelet rich plasma gel) in experimentally induced corneal alkali burns in rats

Ebrahim

Mohammed

Dessouky

et al. 2017

Egyptian Journal of Histology

View full text Add to dashboard Cite

Background: Corneal alkali burns are common ophthalmic emergencies which may lead to permanent visual impairment. In spite of great advances made in the treatment of these cases, the structural and functional restoration of corneal alkali burns remains challenging. Bone marrow-derived mesenchymal stem cells (BM-MSCs) have been demonstrated to enhance corneal healing. However the problem remains in finding a suitable vehicle to deliver BM-MSCs to the site of injury. Objectives: To evaluate the therapeutic potential of BM-MSCs loaded on two biological vehicles (amniotic membrane versus platelet-rich plasma gel) in corneal alkali burns. Materials & Methods: Fifty rats were randomly divided into five equal groups. Group I (control group), group II (corneal ulcer group) and group III (recovery group). Group IV rats with corneal ulcers were treated with amniotic membrane loaded with BM-MSCs while in the group V the rats with corneal ulcers were treated with platelet-rich plasma cells loaded with BMMSCs. The corneal specimens were processed for the light microscope, transmission electron microscope examination, and RT qPCR assay for VEGF and iNOS. Results: Group II showed epithelial separation and denudation with disorganized stroma and disrupted Descemet's membrane and endothelium. Group III showed residual epithelial separation and vacuolation with neovascularization of the disorganized stroma. Group IV showed thinning of corneal epithelium with inflammatory infiltration of the stroma. Group V corneas revealed restoration of corneal epithelial thickness, organized stroma and continuous Descemet's membrane. Also, there was an upregulation of iNOS & VEGF gene expression in groups II and III which was downregulated in group IV and V. Conclusions: The combination of BM-MSCs with PRP gel was a promising treatment for corneal alkali burns and may be applicable for other types of corneal disorders.

show abstract

“…As they hold the possible risk of infection transfer, variable tissue quality, and limited transparency, alternative seeding scaffolds have been proposed [43] .…”

Section: Discussionmentioning

confidence: 99%

The potential therapeutic effect of stem cells loaded on two different vehicles (amniotic membrane and platelet rich plasma gel) in experimentally induced corneal alkali burns in rats

Ebrahim

Mohammed

Dessouky

et al. 2017

Egyptian Journal of Histology

View full text Add to dashboard Cite

show abstract

“…Only seven clinical studies (substudies excluded) involving transplantation of ex vivo cultured LEC have applied intact AM (Table ) as a culture substrate, whereas 29 clinical studies used denuded AM to culture LEC (Table ).…”

Section: Culture Techniques and Use Of Intact And Denuded Ammentioning

confidence: 99%

Concise Review: Altered Versus Unaltered Amniotic Membrane as a Substrate for Limbal Epithelial Cells

Utheim

Salvanos

et al. 2018

Stem Cells Translational Medicine

View full text Add to dashboard Cite

Limbal stem cell deficiency (LSCD) can result from a variety of corneal disorders, including chemical and thermal burns, infections, and autoimmune diseases. The symptoms of LSCD may include irritation, epiphora, blepharospasms, photophobia, pain, and decreased vision. There are a number of treatment options, ranging from nonsurgical treatments for mild LSCD to various forms of surgery that involve different cell types cultured on various substrates. Ex vivo expansion of limbal epithelial cells (LEC) involves the culture of LEC harvested either from the patient, a living relative, or a cadaver on a substrate in the laboratory. Following the transfer of the cultured cell sheet onto the cornea of patients suffering from LSCD, a successful outcome can be expected in approximately three out of four patients. The phenotype of the cultured cells has proven to be a key predictor of success. The choice of culture substrate is known to affect the phenotype. Several studies have shown that amniotic membrane (AM) can be used as a substrate for expansion of LEC for subsequent transplantation in the treatment of LSCD. There is currently a debate over whether AM should be denuded (i.e., de‐epithelialized) prior to LEC culture, or whether this substrate should remain intact. In addition, crosslinking of the AM has been used to increase the thermal and mechanical stability, optical transparency, and resistance to collagenase digestion of AM. In the present review, we discuss the rationale for using altered versus unaltered AM as a culture substrate for LEC. Stem Cells Translational Medicine 2018;7:415–427

show abstract

“…In the majority of these trials, the source was autologous in origin with a concentration varying between 3 to 5%, while in one of the trials a clinical grade AB serum was used [35]. Eleven studies reported the use of FBS [11, 13, 14, 20, 23–25, 27, 31, 33, 36], and 4 studies included both types of serum or switched from FBS to AS [5, 17, 21, 29]. One study did not describe their use of serum in their protocol [10].…”

Section: Culture Processmentioning

confidence: 99%

“…One report exists of a sterile hypopyon after repeated transplantation of human amniotic membrane on cornea surface, probably related to a localized immunoreaction [62]. Twenty-five of the 32 clinical trials reported (78,1%) used human amniotic membrane as a culture substrate [5–7, 9, 12, 14–21, 25–36]. No events of disease transmission related to the use of HAM were reported.…”

Section: Culture Processmentioning

confidence: 99%

See 1 more Smart Citation

Safety of Cultivated Limbal Epithelial Stem Cell Transplantation for Human Corneal Regeneration

Behaegel

Dhubhghaill

Koppen

et al. 2017

Stem Cells International

View full text Add to dashboard Cite

Ex vivo cultivated limbal stem cell transplantation is a promising technique for the treatment of limbal stem cell deficiency. While the results of the clinical trials have been extensively reported since the introduction of the technique in 1997, little has been reported regarding the potential health risks associated with production processes and transplantation techniques. Culture procedures require the use of animal and/or human-derived products, which carry the potential of introducing toxic or infectious agents through contamination with known or unknown additives. Protocols vary widely, and the risks depend on the local institutional methods. Good manufacturing practice and xeno-free culture protocols could reduce potential health risks but are not yet a common practice worldwide. In this review, we focus on the safety of both autologous- and allogeneic-cultivated limbal stem cell transplantation, with respect to culture processes, surgical approaches, and postoperative strategies.

show abstract

Unilateral Partial Limbal Stem Cell Deficiency: Contralateral Versus Ipsilateral Autologous Cultivated Limbal Epithelial Transplantation

Cited by 41 publications

References 16 publications

The potential therapeutic effect of stem cells loaded on two different vehicles (amniotic membrane and platelet rich plasma gel) in experimentally induced corneal alkali burns in rats

The potential therapeutic effect of stem cells loaded on two different vehicles (amniotic membrane and platelet rich plasma gel) in experimentally induced corneal alkali burns in rats

Concise Review: Altered Versus Unaltered Amniotic Membrane as a Substrate for Limbal Epithelial Cells

Safety of Cultivated Limbal Epithelial Stem Cell Transplantation for Human Corneal Regeneration

Contact Info

Product

Resources

About