Unimolecular, Bimolecular, and Intramolecular Hydrolysis Mechanisms of 4-Nitrophenyl β-<scp>d</scp>-Glucopyranoside

Activity-based protein profiling (ABPP) has emerged as a versatile biochemical method for studying enzyme activity under various physiological conditions, with applications so far mainly in biomedicine. Here, we show the potential of ABPP in the discovery of biocatalysts from the thermophilic and lignocellulose-degrading white rot fungus Phanerochaete chrysosporium. By employing a comparative ABPP-based functional screen, including a direct profiling of wood substrate-bound enzymes, we identify those lignocellulose-degrading carbohydrate esterase (CE1 and CE15) and glycoside hydrolase (GH3, GH5, GH16, GH17, GH18, GH25, GH30, GH74 and GH79) enzymes specifically active in presence of the substrate. As expression of fungal enzymes remains challenging, our ABPP-mediated approach represents a preselection procedure for focusing experimental efforts on the most promising biocatalysts. Furthermore, this approach may also allow the functional annotation of domains-of-unknown functions (DUFs). The ABPP-based biocatalyst screening described here may thus allow the identification of active enzymes in a process of interest and the elucidation of novel biocatalysts that share no sequence similarity to known counterparts.

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A multiple linear regression approach to the estimation of carboxylic acid ester and lactone alkaline hydrolysis rate constants

Lazare

Tebes-Stevens

Weber

2023

SAR and QSAR in Environmental Research

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Unimolecular, Bimolecular, and Intramolecular Hydrolysis Mechanisms of 4-Nitrophenyl β-d-Glucopyranoside

Abstract: Glycosides hydrolyze through different mechanisms at different pH values, but systematic studies are lacking. Here we report the pH-rate constant profile for the hydrolysis of 4-nitrophenyl β-D-glucoside. An

Cited by 3 publications

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A novel “Turn-on” fluorometric assays triggered reaction for β-glucosidase activity based on quercetin derived silicon nanoparticles and its potential use for cell imaging

A novel “Turn-on” fluorometric assays triggered reaction for β-glucosidase activity based on quercetin derived silicon nanoparticles and its potential use for cell imaging

Identification of fungal lignocellulose-degrading biocatalysts secreted by Phanerochaete chrysosporium via activity-based protein profiling

A multiple linear regression approach to the estimation of carboxylic acid ester and lactone alkaline hydrolysis rate constants

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