Union is strength: matrix elasticity and microenvironmental factors codetermine stem cell differentiation fate

Lv, Hong; Li, Lisha; Zhang, Yin; Chen, Zhishen; Sun, Miao; Xu, Tongge; Tian, Lei; Lu, Man; Ren, Min; Liu, Yuanyuan; Li, Yulin

doi:10.1007/s00441-015-2190-z

Cited by 17 publications

(6 citation statements)

References 87 publications

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“…Thus, the gelation time result indicated that the SA 2 ColI 1 may reduce the MSCs loss and provide better protection compared with SA 4 ColI 1 and SA. The rheological behavior of the hydrogels was investigated via detecting their modulus of elasticity, and the modulus values of all the hydrogels ranged from 200 Pa to 1000 Pa (Figure 3b), which were similar to the human body's tissue modulus values, and this will contribute to the interaction of the implants and the focal tissue and improve the recovery of the damaged organs (14). Wherein, the SA 2 ColI 1 presented a stable modulus values curve compared to SA 4 ColI 1 and SA, which obviously made better matching degree for the tissues and may be more beneficial to the tissue repair and regeneration.…”

Section: Resultsmentioning

confidence: 85%

Preparing an injectable hydrogel with sodium alginate and Type I collagen to create better MSCs growth microenvironment

Zhou

Zhang

et al. 2019

E-Polymers

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In the past few decades, stem cell transplantation has been generally accepted as an effective method on the treatment of tissue and organ injury. However, the insufficient number of transplanted stem cells and low survival rate that caused by series of negative conditions limit the therapeutic effect. In this contribution, we developed an injectable hydrogel composed of sodium alginate (SA) and Type I collagen (ColI), as the tissue scaffold to create better growth microenvironment for the stem cells. Compared the traditional SA scaffold, the ColI/SA hydrogel inherits its biomimetic properties, and simultaneously has shorter gelation time which means less loss of the transplanted stem cells. The mesenchyma stem cell (MSC) culture experiments indicated that the ColI/SA hydrogel could prevent the MSC apoptosis and contributed to faster MSC proliferation. It is highlighted that this ColI/SA hydrogel may have potential application for tissue regeneration and organ repair as the stem cell scaffold.

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Section: Resultsmentioning

confidence: 85%

Preparing an injectable hydrogel with sodium alginate and Type I collagen to create better MSCs growth microenvironment

Zhou

Zhang

et al. 2019

E-Polymers

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“…Different stiffness hydrogels can affect the behavior of stem cells, MSCs, fibroblasts ( Ibañez et al, 2021 ), macrophages, and other cells, thereby affecting the process of tissue repair or bone defect. In particular, hydrogels affect the phenotype of macrophages through changes in stiffness ( Lv et al, 2015 ), leading to changes in tissue inflammatory environment and angiogenesis, and ultimately promoting wound healing. How hydrogels affect the expression of related proteins or oxidative stress, leading to changes in cell behavior has become a research hotspot in recent years.…”

Section: Effects Of Hydrogel Stiffness On Tissue Repairmentioning

confidence: 99%

A Review on the Design of Hydrogels With Different Stiffness and Their Effects on Tissue Repair

Luo

Tan

Zhu

et al. 2022

Front. Bioeng. Biotechnol.

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Tissue repair after trauma and infection has always been a difficult problem in regenerative medicine. Hydrogels have become one of the most important scaffolds for tissue engineering due to their biocompatibility, biodegradability and water solubility. Especially, the stiffness of hydrogels is a key factor, which influence the morphology of mesenchymal stem cells (MSCs) and their differentiation. The researches on this point are meaningful to the field of tissue engineering. Herein, this review focus on the design of hydrogels with different stiffness and their effects on the behavior of MSCs. In addition, the effect of hydrogel stiffness on the phenotype of macrophages is introduced, and then the relationship between the phenotype changes of macrophages on inflammatory response and tissue repair is discussed. Finally, the future application of hydrogels with a certain stiffness in regenerative medicine and tissue engineering has been prospected.

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“…BMMSCs are of great interest for biomedical research, drug discovery, and cell-based therapies as they are capable of differentiating into neurogenic, adipogenic, myogenic, and osteogenic lineages 1 - 3 . The fate of the stem cells is influenced by the microenvironment in which they reside 4 . Although extensive efforts are devoted to identifying biochemical factors that mimic the stem cell microenvironment to maintain the stem status and to promote the differentiation if necessary, it is still a challenge to optimize new biomolecules supporting stem cell differentiation and/or producing a high level of desired lineages from the stem cells.…”

Section: Introductionmentioning

confidence: 99%

Effects of Matrix Stiffness on the Morphology, Adhesion, Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells

et al. 2018

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BMMSCs have drawn great interest in tissue engineering and regenerative medicine attributable to their multi-lineage differentiation capacity. Increasing evidence has shown that the mechanical stiffness of extracellular matrix is a critical determinant for stem cell behaviors. However, it remains unknown how matrix stiffness influences MSCs commitment with changes in cell morphology, adhesion, proliferation, self-renewal and differentiation. We employed fibronectin coated polyacrylamide hydrogels with variable stiffnesses ranging from 13 to 68 kPa to modulate the mechanical environment of BMMSCs and found that the morphology and adhesion of BMMSCs were highly dependent on mechanical stiffness. Cells became more spread and more adhesive on substrates of higher stiffness. Similarly, the proliferation of BMMSCs increased as stiffness increased. Sox2 expression was lower during 4h to 1 week on the 13-16 kPa and 62-68 kPa, in contrast, it was higher during 4h to 1 week on the 48-53 kPa. Oct4 expression on 13-16 kPa was higher than 48-53 kPa at 4h, and it has no significant differences at other time point among three different stiffness groups. On 62-68 kPa, BMMSCs were able to be induced toward osteogenic phenotype and generated a markedly high level of RUNX2, ALP, and Osteopontin. The cells exhibited a polygonal morphology and larger spreading area. These results suggest that matrix stiffness modulates commitment of BMMSCs. Our findings may eventually aid in the development of novel, effective biomaterials for the applications in tissue engineering.

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Union is strength: matrix elasticity and microenvironmental factors codetermine stem cell differentiation fate

Cited by 17 publications

References 87 publications

Preparing an injectable hydrogel with sodium alginate and Type I collagen to create better MSCs growth microenvironment

Preparing an injectable hydrogel with sodium alginate and Type I collagen to create better MSCs growth microenvironment

A Review on the Design of Hydrogels With Different Stiffness and Their Effects on Tissue Repair

Effects of Matrix Stiffness on the Morphology, Adhesion, Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells

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