Unique aerodynamically driven methodology for forming droplets, threads to scaffolds

Abstract

Jet-based methodologies for forming droplets and threads leading to the fabrication of scaffolds are playing a pivotal role in materials science and engineering. These techniques are essentially interfacing engineering with the biological sciences. There are several routes for forming droplets, which are subsequently deposited with precision, namely, ink-jet printing to electrospraying. Fabricating threads/fibers sized from the nano-to the micrometer range are generally carried out by means of electrospinning. These three processes are well-established and have been explored to a great extend. In this article, we have shown a unique processing technology completely driven by a pressure difference over an orifice possessing the ability to form droplets, threads to scaffolds. Hence, this process now joins the microfabrication race alongside its competing technologies, in the hope to undergo a rapid developmental program arming itself for competing with its rival technologies.

A novel direct aerodynamically assisted threading methodology for generating biologically viable microthreads encapsulating living primary cells

“…5) demonstrated a variation in capsulated cellular populations as cellular beads along the thread. Exposing the substrate to a longer time to the bio‐threading processing gave rise to a bio‐scaffold much like those previously observed 7, 18…”

Living Scaffolds (Specialized and Unspecialized) for Regenerative and Therapeutic Medicine

“…There are essentially three needle-based continuous fiber preparation routes, which have been elucidated for forming advanced composite fibers containing materials. These are electrospinning (ES), , aerodynamically assisted threading (AAT), , and, very recently, pressure-assisted spinning (PAS). , In this review the authors will endeavor to discuss the advantages and disadvantages of each needle-assisted fiber generation method in comparison to each other, followed by a discussion of their applicability to the life sciences.…”

Bio-protocols for directly forming active encapsulations containing living primary cells

“…31 However, it was only very recently that this approach was investigated for jetting media having particulate systems, which demonstrated AAJ as a novel materials processing science as applied to the jetting of structural and functional materials. [32][33][34][35] More importantly we discovered that living primary cells could be directly jetted by means of AAJ. 36 Similarly in some respects, co-flow employs by-pass media flow within a bath of liquid for drawing out media flowing within a needle.…”