Unique mitochondrial genome structure of the green algal strain YC001 (Sphaeropleales, Chlorophyta), with morphological observations

Lee, Hyung-Gwan; Song, Hae Jung; Kim, Dae-Soo; Cho, Chung Hyun; La, Hyun-Joon; Oh, Hee‐Mock; Yoon, Hwan Su

doi:10.2216/15-71.1

Cited by 9 publications

(7 citation statements)

References 23 publications

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“…F50 (Hu et al 2013 ); and Coelastrella sp. YC001 (Lee et al 2016 ). The presence of longitudinal ribs is a clear character that helps to distinguish among genera; small thickenings at the poles, and a citriforme until globose morphology, are also considered relevant taxonomic characters (Table 1 ).…”

Section: Discussionmentioning

confidence: 99%

“…Several morphological, ultrastructural and reproductive characteristics are shared (or appear to be) between these coccoid green microalgae (Table 1 ), therefore misidentification has been a problem for further phylogenetic studies. Several sequences available online carried misleading names and complicates the analysis of the group’s phylogeny (see Kaufnerová and Eliáš 2013 ; Lee et al 2016 ). As mentioned, certain genera, as Scotiella , were found to be resistant stages (which presented a similar morphology to Coelastroideae: non-motile cysts with longitudinal ribs on cell surface) of other green algae as Chlamydomonadales i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Members of the subfamily Coelastroideae have been previously placed in the families Oocystaceae, Chlorellaceae, and Scotielloideae based on shape, reproduction, cell wall morphology and composition (Kalina and PunČochářová 1987 ), until phylogenetic molecular studies were applied. Nowadays, DNA amplification and sequencing of the 18S rDNA and internal transcribed spacer (ITS) studies placed Coelastroideae within the family Scenedesmaceae, order Sphaeropleales (Hanagata 1998 , 2001 ; Hegewald and Hanagata 2000 , 2002 ; Hegewald et al 2010 ; Kaufnerová and Eliáš 2013 ; Lee et al 2016 ; Ancona-Canché et al 2017 ). According to AlgaeBase (Guiry and Guiry 2020 ), the subfamily Coelastroideae comprises seven genera: Coelastrella Chodat 1922 (16 species flagged as accepted taxonomically), Scotiellopsis Vinatzer 1975 (1 sp.…”

Section: Introductionmentioning

confidence: 99%

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Revision of Coelastrella (Scenedesmaceae, Chlorophyta) and first register of this green coccoid microalga for continental Norway

Goecke

Noda

Paliocha

et al. 2020

World J Microbiol Biotechnol

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A terrestrial green microalga was isolated at Ås, in Akershus County, Norway. The strain corresponded to a coccoid chlorophyte. Morphological characteristics by light and electron microscopy, in conjunction with DNA amplification and sequencing of the 18 s rDNA gene and ITS sequences, were used to identify the microalgae. The characteristics agree with those of the genus Coelastrella defined by Chodat, and formed a sister group with the recently described C. thermophila var. globulina. Coelastrella is a relatively small numbered genus that has not been observed in continental Norway before; there are no previous cultures available in collections of Norwegian strains. Gas chromatography analyses of the FAME-derivatives showed a high percentage of polyunsaturated fatty acids (44–45%) especially linolenic acid (C18:3n3; 30–34%). After the stationary phase, the cultures were able to accumulate several carotenoids as neoxanthin, pheophytin a, astaxanthin, canthaxanthin, lutein, and violaxanthin. Due to the scarcity of visual characters suitable for diagnostic purposes and the lack of DNA sequence information, there is a high possibility that species of this genus have been neglected in local environmental studies, even though it showed interesting properties for algal biotechnology.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Revision of Coelastrella (Scenedesmaceae, Chlorophyta) and first register of this green coccoid microalga for continental Norway

Goecke

Noda

Paliocha

et al. 2020

World J Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…subcapitata belongs to the family Selenastraceae, order Sphaeropleales, but its phylogenetic position in the group has not been resolved 4 , 21 , 22 . Although well-resolved phylogenetic relationships have been recently reported for the Sphaeropleales, using plastid or mitochondrial genome-encoded proteins 19 , 20 , 23 , 24 , the organellar genomes of R . subcapitata have not been sequenced.…”

Section: Introductionmentioning

confidence: 99%

Raphidocelis subcapitata (=Pseudokirchneriella subcapitata) provides an insight into genome evolution and environmental adaptations in the Sphaeropleales

Suzuki

Yamaguchi

Nakajima

et al. 2018

Sci Rep

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The Sphaeropleales are a dominant group of green algae, which contain species important to freshwater ecosystems and those that have potential applied usages. In particular, Raphidocelis subcapitata is widely used worldwide for bioassays in toxicological risk assessments. However, there are few comparative genome analyses of the Sphaeropleales. To reveal genome evolution in the Sphaeropleales based on well-resolved phylogenetic relationships, nuclear, mitochondrial, and plastid genomes were sequenced in this study. The plastid genome provides insights into the phylogenetic relationships of R. subcapitata, which is located in the most basal lineage of the four species in the family Selenastraceae. The mitochondrial genome shows dynamic evolutionary histories with intron expansion in the Selenastraceae. The 51.2 Mbp nuclear genome of R. subcapitata, encoding 13,383 protein-coding genes, is more compact than the genome of its closely related oil-rich species, Monoraphidium neglectum (Selenastraceae), Tetradesmus obliquus (Scenedesmaceae), and Chromochloris zofingiensis (Chromochloridaceae); however, the four species share most of their genes. The Sphaeropleales possess a large number of genes for glycerolipid metabolism and sugar assimilation, which suggests that this order is capable of both heterotrophic and mixotrophic lifestyles in nature. Comparison of transporter genes suggests that the Sphaeropleales can adapt to different natural environmental conditions, such as salinity and low metal concentrations.

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“…There are already established species-specific methods for the induction of an axenic culture by serial diluted plating, treatment with antibiotic cocktails, and cryopreservation using a programmed freezing rate and cold shock treatments 15,16 . Moreover, its whole genome sequence has been analysed and annotated accurately 17 . Thus, by establishing a genetic transformation system for Ettlia sp.…”

mentioning

confidence: 99%

Development of a species-specific transformation system using the novel endogenous promoter calreticulin from oleaginous microalgae Ettlia sp.

Lee

et al. 2020

Sci Rep

Self Cite

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Microalgae not only serve as raw materials for biofuel but also have uses in the food, pharmaceutical, and cosmetic industries. However, regulated gene expression in microalgae has only been achieved in a few strains due to the lack of genome information and unstable transformation. This study developed a species-specific transformation system for an oleaginous microalga, Ettlia sp. YC001, using electroporation. The electroporation was optimized using three parameters (waveform, field strength, and number of pulses), and the final selection was a 5 kV cm −1 field strength using an exponential decay wave with one pulse. A new strong endogenous promoter CRT (Pcrt) was identified using transcriptome and quantitative PCR analysis of highly expressed genes during the late exponential growth phase. The activities of this promoter were characterized using a codon optimized cyan fluorescent protein (CFP) as a reporter. The expression of CFP was similar under Pcrt and under the constitutive promoter psaD (PpsaD). The developed transformation system using electroporation with the endogenous promoter is simple to prepare, is easy to operate with high repetition, and utilizes a species-specific vector for high expression. This system could be used not only in molecular studies on microalgae but also in various industrial applications of microalgae.

show abstract

Unique mitochondrial genome structure of the green algal strain YC001 (Sphaeropleales, Chlorophyta), with morphological observations

Cited by 9 publications

References 23 publications

Revision of Coelastrella (Scenedesmaceae, Chlorophyta) and first register of this green coccoid microalga for continental Norway

Revision of Coelastrella (Scenedesmaceae, Chlorophyta) and first register of this green coccoid microalga for continental Norway

Raphidocelis subcapitata (=Pseudokirchneriella subcapitata) provides an insight into genome evolution and environmental adaptations in the Sphaeropleales

Development of a species-specific transformation system using the novel endogenous promoter calreticulin from oleaginous microalgae Ettlia sp.

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