Unique Populations of Sulfur-Oxidizing Bacteria in Natural Cold Sulfur Springs in Slovakia

Nosalova, Lea; Fecskeová, Lívia Kolesár; Piknová, Mária; Bónová, Katarína; Pristaš, Peter

doi:10.1080/01490451.2023.2167021

Cited by 7 publications

(8 citation statements)

References 52 publications

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“…Among the five sulfur springs studied, genera Thiothrix and Sulfurovum constituted the majority of obtained sequences. Thiothrix genus was identified using a noncultivation approach among other cold sulfur springs in Slovakia [ 44 , 45 ]. Contrarily, this is the first evidence of the Sulfurovum genus constituting a large part of the microbiota of Slovak sulfur springs.…”

Section: Discussionmentioning

confidence: 99%

Thiothrix and Sulfurovum genera dominate bacterial mats in Slovak cold sulfur springs

Nosalova,

Mekadim,

Mrazek

et al. 2023

Environmental Microbiome

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Microbiota of sulfur-rich environments has been extensively studied due to the biotechnological potential of sulfur bacteria, or as a model of ancient life. Cold terrestrial sulfur springs are less studied compared to sulfur-oxidizing microbiota of hydrothermal vents, volcanic environments, or soda lakes. Despite that, several studies suggested that sulfur springs harbor diverse microbial communities because of the unique geochemical conditions of upwelling waters. In this study, the microbiota of five terrestrial sulfur springs was examined using a 16 S rRNA gene sequencing. The clear dominance of the Proteobacteria and Campylobacterota phyla of cold sulfur springs microbiota was observed. Contrary to that, the microbiota of the hot sulfur spring was dominated by the Aquificota and Firmicutes phylum respectively. Sulfur-oxidizing genera constituted a dominant part of the microbial populations with the Thiothrix and Sulfurovum genera identified as the core microbiota of cold sulfur terrestrial springs in Slovakia. Additionally, the study emphasizes that sulfur springs in Slovakia support unique, poorly characterized bacterial communities of sulfur-oxidizing bacteria.

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Section: Discussionmentioning

confidence: 99%

Thiothrix and Sulfurovum genera dominate bacterial mats in Slovak cold sulfur springs

Nosalova,

Mekadim,

Mrazek

et al. 2023

Environmental Microbiome

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“…A variety of sulfur oxidizing bacteria use sulfur as an energy source, and this group encompasses bacteria with little or no taxonomic relationship to each other. Aerobic sulfur-oxidizing bacteria, so-called colorless sulfur-oxidizing bacteria, are mainly mesophilic (Nosalova et al 2023). Sulfur-oxidizing bacteria from post-coal mining pond water were isolated using enrichment culture technique in the thiosulfate mineral media.…”

Section: Figure 2 the Potency Of Sob For Decreased Sulfur Concentrati...mentioning

confidence: 99%

“…In addition to the sulfur dissimilation pathways of representatives of the Archaea domain, there are currently at least three well-described pathways postulated for use by representatives of the Bacteria domain. These include the complete oxidation of reduced sulfur compounds to sulfate end products, known as the Sox metabolic pathway, the tetrathionate (S4I) pathway involving polythionate intermediates typical for acidophilic bacteria, and the branched thiosulfate oxidation pathway (Konrad et al 2023;Nosalova et al 2023). Green sulfur bacteria use various reduced sulfur compounds such as sulfide, elemental sulfur, and thiosulfate as electron donors for photoautotrophic growth.…”

Section: The Potency Of Potential Sob Isolates In the Various Of Thio...mentioning

confidence: 99%

Screening, Identification, and Optimization of Sulfur-Oxidizing Bacteria from Post-coal Mining Ponds of Different Ages in Samarinda, East Kalimantan, Indonesia

2024

Global NEST: The International Journal

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<p>This research was conducted to isolation, screening, identification, and culture conditions optimization (pH, temperature, thiosulfate concentration, and incubation period) of potential sulfur-oxidizing bacterial (SOB) to reduce sulfur concentrations in post-coal mining ponds water of various ages located in Samarinda, East Kalimantan, Indonesia. The ponds used in this study were ponds < 5 years old and > 20 years old. The research obtained seventy-five bacteria isolates, among them nine isolates can increase the pH of thiosulfate broth media. Among the nine bacteria isolates, three isolates KT1.8, KT1.9, and KT1.13 have the highest potency to reduce sulfur concentration of media were 6%, 148%, and 101%. Based on the similarity of the 16S rDNA sequences, the KT1.8, KT1.9, and KT1.13 isolates were identified as Priestia qingshengii HLS-7 (98.9%), Sphingobacterium siyangensis DS48 (97.6%), and Pseudomonas putida CFBP 4966 (98.3%). The three potential SOB isolates grew better at pH 6 at 30° C temperature with the growth of KT1.8 = 146x1014 cell/mL; KT1.9 = 81x107 cell/mL; and KT1.13 = 33x107 cell/mL; decrease in sulfur concentration KT1.8 = 43.57%; KT1.9 = 43.57%; and KT1.13 = 42.48%. On media containing 10 mM and 20 mM thiosulfate on the sixth day of incubation, cell density isolates KT1.8 = 98x1012 cell/mL; KT1.9 = 25x107 cell/mL; and KT1.13 = 85x106 cell/mL; accompanied by a decrease in sulfur concentration of KT1.8 = 46.52%; KT1.9 = 40.40%; and KT1.13 = 43.71% were obtained. Those three bacteria isolate potential can be developed as bioremediation agents of post-coal mining pond water contaminated by sulfur.</p>

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“…Moreover, 3 showed 16S rRNA sequence similarities lower than 97%, and single isolate showed similarity as low as 92.4% [ 128 ]. Similarly, 4 out of 13 DGGE bands in non-cultivation analysis of populations of SOBs in natural cold sulfur springs in Slovakia showed 16S rRNA similarities lower than 97%, in one case with similarity as low as 95% to monotypic bacterial genus Thiofaba [ 129 ]. Similarly, cultivation analysis of springs with high salinity showed that cultivable microbiota were mostly composed of novel, unidentified bacterial species, showing 16S rRNA gene sequence similarity from 97.55% to 98.47% [ 127 ].…”

Section: Main Bodymentioning

confidence: 99%

“…Cold sulfur springs harbor unique, not yet explored bacterial communities [ 125 , 128 , 129 ] and are a neglected source of novel species of sulfur-oxidizing bacteria. Studies of cold sulfur springs, therefore, expand our knowledge of the bacteria involved in the biogeochemical sulfur cycle, their metabolism, ecology, and evolution, and may indicate the relationships between the sulfur microbial communities and the environment they inhabit.…”

Section: Main Bodymentioning

confidence: 99%

Cold Sulfur Springs—Neglected Niche for Autotrophic Sulfur-Oxidizing Bacteria

et al. 2023

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Since the beginning of unicellular life, dissimilation reactions of autotrophic sulfur bacteria have been a crucial part of the biogeochemical sulfur cycle on Earth. A wide range of sulfur oxidation states is reflected in the diversity of metabolic pathways used by sulfur-oxidizing bacteria. This metabolically and phylogenetically diverse group of microorganisms inhabits a variety of environments, including extreme environments. Although they have been of interest to microbiologists for more than 150 years, meso- and psychrophilic chemolithoautotrophic sulfur-oxidizing microbiota are less studied compared to the microbiota of hot springs. Several recent studies suggested that cold sulfur waters harbor unique, yet not described, bacterial taxa.

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Unique Populations of Sulfur-Oxidizing Bacteria in Natural Cold Sulfur Springs in Slovakia

Cited by 7 publications

References 52 publications

Thiothrix and Sulfurovum genera dominate bacterial mats in Slovak cold sulfur springs

Thiothrix and Sulfurovum genera dominate bacterial mats in Slovak cold sulfur springs

Screening, Identification, and Optimization of Sulfur-Oxidizing Bacteria from Post-coal Mining Ponds of Different Ages in Samarinda, East Kalimantan, Indonesia

Cold Sulfur Springs—Neglected Niche for Autotrophic Sulfur-Oxidizing Bacteria

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